Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"the spindle assembly checkpoint (SAC)"”.
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PP2A-B56 isoform specificity at the centromere and kinetochore
… three major mitotic processes are regulated by the PP2A-B56 phosphatase complex: the Spindle Assembly Checkpoint (SAC), kinetochore-microtubule attachments and sister chromatid cohesion. We show here that these key functions of PP2A-B56, which require its localization to either the kinetochore …
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Exploring the effects and efficacy of novel cell cycle regulating compounds in aggressive Breast, Ovarian and Cervical Cancer
The Spindle Assembly Checkpoint (SAC) is a cell signalling system that regulates cell division. SAC ensures the correct separation of chromosomes during mitosis. Defects in SAC can result in genome instability, increasing the risk of cancer development and making it a potential therapeutic target …
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Changes in Kinetochore Structure and Molecular Composition in Response to Mis-attachment
… sister chromatids whose correct segregation to the daughter cells is ensured by amphitelic attachment, in which the two sister kinetochores (KTs) are attached to microtubules (MTs) from opposite mitotic spindle poles. KT mis-attachments can occur in early mitosis and cause chromosome …
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Protein Phosphatase 1 at the Kinetochore Regulates Chromosome Segregation
… attachments are destabilized through the error correction machinery. Second, the spindle assembly checkpoint (SAC) delays anaphase onset until all kinetochores have achieved bioriented microtubule attachments. Both of these mechanisms are mediated by several centromeric and kinetochore …
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Examining the Regulation of Cell Division by the Spindle Assembly Checkpoint
… during cell division is essential for the maintenance of a cell's genomic stability. The molecular surveillance mechanism called the spindle assembly checkpoint (SAC) inhibits chromosome segregation before all chromosomes are correctly attached (bi-oriented) on the microtubule spindle …
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Investigating the consequences of chromosome abnormalities arising during pre-implantation development of the mouse
The majority of human pre-implantation embryos created through in vitro fertilization (IVF) are mosaic as they are constituted of a mixture of diploid and aneuploid cells. Chromosome abnormalities are widely believed to contribute towards the relatively low success rates of IVF treatment. …
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Molecular Mechanisms of Mitotic Checkpoint Complex Assembly onto Kinetochores
… to improper kinetochore-microtubule attachments, the spindle assembly checkpoint (SAC), activates the mitotic checkpoint complex (MCC), to inhibit the E3 ubiquitin ligase, the anaphase-promoting complex/cyclosome (APC/C). Inhibition of the APC/C then delays premature chromosome segregation by …
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Unraveling the multifunctionality of human Spindly
… It was shown to be required for recruitment of the dynein/dynactin motor complex to kinetochores thus to promote chromosome alignment and mitotic progression. Kinetochore recruitment of the dynein/dynactin motor complex is crucial for maturation of kinetochore-microtubule attachments and for …
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Assembly and autoinhibitory mechanisms of the budding yeast kinetochore KMN complex
… mitosis, kinetochore protein complexes form the primary and essential linkage between spindle microtubules and chromosomes. Kinetochores directly mediate chromosome segregation by forming robust attachments to microtubules under applied force, and act to ensure the accuracy of chromosome …
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Regulated Recruitment of the Chromosomal Passenger Complex to Chromatin and Microtubules Promotes Accurate Cell Division
<p>Aurora B, the kinase subunit of the chromosomal passenger complex (CPC), promotes accurate cell division by destabilizing erroneous kinetochoremicrotubule attachments and activating the spindle assembly checkpoint (SAC). Both functions require Aurora B activation, which is promoted by its …
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From the End to the Middle: Regulation of Telomere Length and Kinetochore Assembly by the RNR Inhibitor SML1
… an adequate and balanced supply of dNTPs. In Saccharomyces cerevisiae, de novo dNTP synthesis through nucleotide reduction by the Ribonucleotide reductase (RNR) enzyme is the sole method of production. Hence, RNR activitity is highly regulated via allosteric control, transcriptional control, …
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Identification and characterization of Sgo2 interactions - Insights into dynamic chromosomal localization, mechanism of cohesin protection and putative checkpoint function
Sister chromatids are embraced and held together by a ring-shaped multiprotein complex called cohesin, from the time of their generation in S-phase until their separation in M-phase. Shugoshins protect centromeric cohesin from premature dissociation and, hence, are important regulators of genome …
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The peptidyl-prolyl isomerase Pin1 is required for maintenance of the spindle assembly checkpoint
… during S-phase and segregated during M-phase of the eukaryotic cell cycle. The two sister chromatids of each duplicated chromosome are topologically entrapped and, thus, paired by the ring-shaped protein complex cohesin. They are separated in anaphase of mitosis when cohesin is …
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Regulation of Anaphase Promoting Complex/Cyclosome to Control M Phase Exit
<p>The Anaphase Promoting Complex/Cyclosome (APC/C) is a RING E3 ligase that plays essential roles both within and outside of the cell cycle. At the onset of anaphase, the APC/C targets cyclin B and securin for degradation, initiating chromosome separation and mitotic exit. Regulation of APC/C …