Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"ternary complex"”.
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Defining the mechanisms of microbial sensing among members of the toll-like receptor 2 sub-family
… bind TLR1 and TLR2 resulting in a stable ternary complex that drives intracellular signaling. However, the order of complex formation upon recognition of microbial components is poorly understood. The primary objectives of this dissertation are to define the sequence of events by which …
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The Role of Phosducin-like Protein as a Co-chaperone with the Cytosolic Chaperonin Complex in Assembly of the G Protein βγ Subunit Dimer
… and biochemical studies on the structure of the complex between the cytosolic chaperonin CCT and PhLP. Binding of PhLP to CCT occurs through only one of the two chaperonin rings, making multiple contacts with CCT through both its N- and C-terminal domains. In addition, we show that PhLP acts as a …
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Essais de purification de l'insulin-like growth factor binding protein-3 (IGFBP-3) bovine
… in plasma circulating as part of a 150 kDa ternary complex with one IGF peptide and a single acid-labile subunit. The IGFBP-3 purification would allow the measure of the changes in plasma IGFBP-3 throughout the day and the development and in various physiological situations. We try to purify …
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New insights into the mechanism of dna replication on unmodified and benzo[a]pyrene modified templates using surface plasmon resonance
… and reduction in the levels of KF-DNA complexes formed. Using surface plasmon resonance and an assay that directly examines the impact of the identity of the incoming dNTP on formation of KF-DNA complexes, we present evidence for the formation of a destabilized complex in the presence …
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A multi-level approach to understanding the regulation of translation initiation
mRNA translation is an extremely complex process required for life. Translation consumes vast amounts of cellular resources, and organisms have evolved tight regulatory mechanisms to control this process, which are often deregulated in cancer and other disease states. Initiation, as the …
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Examining the effect of CBP on the E2A-PBX1 and HOXB4 interaction
… by E2A to PBX1 allows E2A-PBX1 to stabilize a ternary complex involving E2A-PBX1, HOX, and CBP resulting in the deregulated expression of critical PBX1 or HOX target genes. I demonstrate using in vitro protein-protein interactions that this ternary complex involving E2A-PBX1, HOXB4 (chosen as a …
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Development of Protein Degraders Inspired by Chemical Modifications
… E3 ligase-recruiting handle and that tighter ternary complex formation is favored. Mechanistic elucidation via inhibitor screening confirmed that the Dha-based degrader operates through the ubiquitin-proteasome system by recruiting a Cullin-RING E3 ligase. Lastly, transferability studies …
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STRATEGIES TO TARGET HUMAN D-ASPARTATE OXIDASE AND MODULATE ENDOGENOUS D-ASPARTATE LEVELS IN SCHIZOPHRENIA
… DASPO and E3 ligases, and for the formation of ternary complexes necessary for DASPO proteasomal degradation. Among them, PROTAC 1 showed ternary complex formation with a KD = 1.6 μM and induced hDASPO degradation in a neuroblastoma cell line after 48 hours of treatment. Ternary complex …
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Kinetics of the ligand exchange reactions between bidentate ligands and rriethylenetetramine nickelate (II) and synthesis of boron (III) subphthalocyanines with various boron substitutions
… over the pH range of 5.2 to 8.0 at 25oC. A ternary complex Ni(Trien)(5MP) forms within a few seconds and subsequently reacts with 5MP to form Ni(5MP)3. The reaction is first-order with respect to: [5MP], [Ni(Trien)(5MP)] and [H+]. The rate law is given by:</p> <p>k dt d[Ni(trien)] = ( − MP …
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Biochemical characterization of Aspergillus fumigatus SidA: a flavin-dependent N-hydroxylating enzyme
… experiments indicate that Af SidA forms a ternary complex with NADP+ and L-ornithine for catalysis. Furthermore, in the absence of substrate, Af SidA forms a stable C4a-(hydro)peroxyflavin intermediate that is stable on the second time scale. Af SidA is also inhibited by several halides and …
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Interações da trancinolona com ciclodextrinas em sistemas multicomponentes
… was solubilized forming incredible stable complexes with cyclodextrins, in which bests results were observed applying randomyl-methylated-beta-cyclodextrin (RMβCD) (161 fold on increased solubility). The co-solvents TEA and NMP also enhanced drug solubility 1.4 and 6.7 fold, respectively. …
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DNA-Protein Cross-links: Formation, Repair, and Inhibition of DNA replication
… bulky lesions by influencing the geometry of the ternary complex between DNA template, polymerase, and the incoming dNTP. In this chapter, model hydrolytically stable DpCs were prepared by oxime ligation between 5fC in DNA and oxy-lysine containing peptides. Primer extension products were analyzed …
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NMR application and development : study of the ribosomal RNA-L11 complex
… maritima in its free form was solved, the binary complex of L 11 protein with RNA and the N-terminal domain alignment were investigated, the possible inhibition mode of the L11-RNA complex by thiostrepton was proposed and studies aimed at NMR method development and application were undertaken. The …
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P125, A COPII INTERACTING PROTEIN
Coat protein complex II (COPII) coated vesicles/carriers mediate the export of proteins from the ER via specialized domains known as the ER exit sites (ERES). p125A is a protein of 125 kDa that interacts with Sec23A, a component of the COPII coat. It was found to participate in the organization of …
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Surface Tethered PAMAM Dendrimers On Controlled Pore Glass As Color Release Sensors For Phosphates
… during the IDAs.</p> <p>Indicator-Metal-Ligand ternary complex formation constants were also determined as a reference to the surface works using two colorimetric dyes, bromopyrogallol red (BPR) and pyrocatechol violet (PV). Three metal ions tested were Cu<sup>2+</sup>, Ni<sup>2+</sup>, and …
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Regulation of Ciz1 by Cyclin A-Cyclin Dependent Kinase 2 (CDK2) mediated phosphorylation
… sites at the G1/S phase transition and this ternary complex promotes initiation of DNA replication. However, the mechanisms by which Ciz1 is regulated are not fully understood. Here, Ciz1 protein levels are shown to be reduced after inhibition of CDK activity, suggesting that CDK-mediated …
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Mechanisms underlying substrate engagement by TRIM7 and TRIM21 and strategies for re-directing their E3 ligase activity
… why the formation of a TRIM21:antibody:substrate ternary complex results in the degradation of each component is incompletely understood. I have developed and applied *in vitro* assays to monitor ubiquitination of proteins involved in physiological TRIM21 function and in Trim-Away experiments. In …
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Complexation of Organic Guests and Coordination of Metal Ions by Cyclodextrins: Role of Cyclodextrins in Metal-Guest Interactions
… to the formation of a pollutant/CD/ferrous ion ternary complex which has the ability to produce hydroxyl radicals at the site of bound ferrous ions during Fenton reactions. This results in an increase in hydroxyl radical concentration near the target guest molecule relative to the bulk solution, …
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The Determination of Trace Metals by High Performance Liquid Chromatography with Photometric Detection
… with detection by a pyrocatechol violet ternary complex. The possibility of fluorescent metal detection with 8-hydroxyquinoline-5-sulphonic acid and photometric detection with a water soluble derivative of dithizone is shown.
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