Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"terminal restriction fragment length polymorphism (T-RFLP)"”.
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Characterization of the intestinal microbiome and lactobacilli community: influence of dietary and environmental factors
… were discussed. In Chapter 3, a new protocol of Terminal restriction fragment length polymorphism (T-RFLP) analysis was developed to facilitate lactobacilli composition study. In Chapter 4, both culture dependent methods and the novel T-RFLP tools were used to reveal the impact of piglet age and …
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Determining fecal bacterial profiles of a human-habituated wild chimpanzee population in Mahale Mountains National Park, Tanzania
… and four adults. We analyzed fecal samples using Terminal-Restriction Fragment Length Polymorphism (T-RFLP) of amplified 16S rRNA genes to determine bacterial diversity present. Between 1 and 14 terminal-restriction fragments (T-RFs) were observed in each sample. A total of 26 unique T-RFs were …
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DEPTH AND TIME RELATED VARIATIONS OF MICROBIAL COMMUNIITES IN AN EMERGENT FRESHWATER WETLAND
… of the microbial community structure via Terminal Restriction Fragment Length Polymorphism (T-RFLP) of 16S-rRNA. Analysis of variance (ANOVA) found significant differences were found between the environmental parameters in regards to site, depth, and season. Three physical-chemical …
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Stream microbial communities along an agricultural gradient
… on the extracted sediment and pore-water DNA: terminal restriction fragment length polymorphism (T-RFLP) on the small ribosomal subunit 16S rRNA assessed microbial diversity; and quantitative PCR (qPCR) on amoA, a subunit of the nitrifying gene ammonia monooxygenase, measured nitrifier …
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Microbial diversity and groundwater chemistry in a pristine aquifer
… both the suspended and attached communities with terminal restriction fragment length polymorphism (T-RFLP) and large-scale sequencing of the 16S rRNA gene. Using multivariate statistics, we quantified how the distribution of bacterial populations corresponds to the geochemical zonation of …
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Microbial community dynamics in denitrifying biofilters receiving agricultural drainage
… bacteria community structure was determined by Terminal Restriction Fragment Length Polymorphism (T-RFLP) of nosZ (one of the nitrous oxide reductase genes), and fungal ARISA (FARISA) was used to determine fungal community structure. Spatial and temporal results from the biofilter studies have …
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Development and application of a microbial reliability model to analyze denitrifying biofilter stability
… microbial fingerprinting techniques such as terminal restriction fragment length polymorphism (T-RFLP). This allowed us to get a better understanding of how to model the microbial populations as functional components in the system. In order to quickly and easily apply these methods to …
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Biogeochemical Transformations of Trace Element Pollutants During Coal Combustion Product Disposal
… community in the sediment-ash microcosms using Terminal Restriction Fragment Length Polymorphism (T-RFLP), Quantitative Polymerase Chain Reaction (q-PCR), and Reverse Transcription-qPCR (RT-qPCR). Coal ash did not appear to cause significant changes to the structure of the overall bacterial …
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Bacterial community composition in stream biofilms is influenced by algal response to varying light and phosphorus ratios
Strong correlations between bacterial communities and algal seasonal succession have been previously observed. In aquatic systems, dissolved organic carbon derived by algae is an important resource for bacteria. Light and phosphorus availability are two factors that influence biomass and abundance …
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Early nutrition affects intestinal development and immune response in the neonatal piglet
… Bacterial diversity was evaluated using Terminal Restriction Fragment Length Polymorphism (T-RFLP). There was no effect of route on this parameter. However, day did have an effect with the colonic communities separating out by day. Bacterial densities in the ileum and AC were quantified …