Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"template switching"”.
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Structural determinants of murine leukemia virus reverse transcriptase that are important for template switching, fidelity, and drug resistance
… of RTs are important for fidelity, frequency of template switching, and drug-resistance. First, we developed an in vivo assay and performed mutational analysis of manne leukemia virus (MLV) RT to identify structural elements important for template switching. Based on obtained results, we proposed …
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Conserved recombination patterns across coronavirus subgenera
… are likely major determinants of where the template-switching events that yield recombination breakpoints most commonly occur, it is evident that selection against misfolded recombinant proteins also strongly impacts observable recombination breakpoint distributions in coronavirus genomes …
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The Molecular Mechanism of Break Induced Replication
… end of the broken chromosome into a homologous template followed by processive replication of DNA from the donor molecule. BIR is a key cellular process and is implicated in the restart of collapsed replication forks and several chromosomal instabilities. Recently, our lab demonstrated that the …
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An Alternative View of the Templates and Functions of RNA Polymerase II
… II (pol II) has been implicated in the RNA-templated RNA synthesis during replication of some Viroids and Hepatitis Delta Virus (HDV). In this study HDV RNA-templated pol II transcription was examined in vitro, using HeLa cell nuclear extracts (NE). Under standard conditions for the …
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Structures, Functions, and Druggability of the SARS-CoV-2 Replication–Transcription Complex
… with an opposing polarity to the RdRp on the template RNA (t-RNA) strand. In chapter III, we investigated the effect of this configuration on the RdRp and identified that nsp13 precipitates RdRp backtracking, which is a universal regulatory feature of many nucleic acid polymerases that …
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Statistical analysis of short template switch mutations in human genomes
… rearrangements arise in human genomes through template switch mutations, which occur during DNA replication when there is a transient polymerase switch to an alternate template nearby in three-dimensional space. These variants are routinely captured at kilobase-to-megabase scales in studies of …