Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"temperature jump"”.
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Temperature-jump 2D IR spectroscopy to study protein conformational dynamics
Temperature-jump (T-jump) two-dimensional infrared spectroscopy (2D IR) is developed, characterized, and applied to the study of protein folding and association. In solution, protein conformational changes span a wide range of timescale from nanoseconds to minutes. Ultrafast 2D IR spectroscopy …
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Ultrafast Temperature Jump Study of Energy Transfer in Dye-Doped Polymer Films
… is deposited in a molecular solid sample and the temperature is accurately measured. This technique, termed ultrafast optical calorimetry, permits the generation and measurement of molecular hot spots of several hundred degrees and bulk temperature jumps exceeding one hundred degrees at a heating …
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Ultrafast infrared spectroscopy investigation of DNA-ligand interactions
… non-equilibrium conditions using a laser-induced temperature jump. This chapter is part of preliminary work to develop a temperature-jump, 2D-IR experiment in the future.
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Exploring Downhill Protein Folding Free Energy Landscapes
… kBT, is investigated by combining laser induced temperature-jump kinetics measurements and Langevin dynamics simulations. A kinetic and thermodynamic survey of multiple WW domain mutants is performed in combination with a model to reveal the correlation between protein stability and downhill …
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Direct observation of fast protein folding: Distinct nanosecond and microsecond events in the folding of apomyoglobin
… of horse apomyoglobin are followed by a new temperature-jump fluorescence technique on a nanosecond to 0.5 millisecond time scale in vitro. Collapse to a compact state is complete in under 20 microseconds under strongly-nativizing conditions. The intrinsic tryptophan fluorescence (residue 14 …
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Laser-assisted single-molecule refolding
… FRET detection with laser induced temperature jump. We have calibrated the magnitude of the temperature jump with 1˚C accuracy using gold micro-size sensor. The accuracy of temperature calibration was confirmed by close agreement between single-molecule and bulk DNA duplex melting …
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Early Events in the Folding of the Protein Ubiquitin
… events have been interrogated by a fast laser temperature-jump technique and by low temperature stopped flow mixing. In the case of the T-Jump, initially cold denatured protein solution is rapidly heated with a 20 nsec heating pulse. The subsequent refolding is monitored by time resolved …
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Thermal unfolding dynamics of proteins probed by nonlinear infrared spectroscopy
… ubiquitin. For transient unfolding studies, the temperature of the solution is rapidly raised by a nanosecond temperature jump (T-jump) laser, which is followed by probing structural changes of proteins with dispersed vibrational echo (DVE) spectroscopy or 2D IR spectroscopy.
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Investigation of the interactions in RNA recognition motif-RNA complexes: small molecule inhibitors and kinetics of dissociation
… process. Analysis of kinetics data obtained by temperature jump and stopped-flow experiments showed that the location of the electrostatic interaction controls the rate of different steps in the complex dissociation pathway. Chapter 4 is a description of a simple and rapid method to detect RNA …
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Protein folding in living cells and under pressure
… of fast-folding reactions following pressure-jump perturbation and pair experiment with molecular dynamics simulations. The first chapter is a review of the effects of pressure on the structure of biomolecules as well as a brief literature review of pressure-probed protein folding kinetics. We …
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Efficient simulation of molecular gas transport for micro- and nanoscale applications
… from equilibrium (low Mach number, small temperature gradients, etc.), which make the traditional particle methods like the direct simulation Monte Carlo (DSMC) computationally inefficient. By considering only the deviation from equilibrium, the low-variance particle method introduced …
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The Effects of Rarefaction and Thermal Non-Equilibrium on a Blunt Body and a Bicone in Hypersonic Flow and Their Shape Optimization for Reducing Both Drag and Heat Transfer
… with hypersonic flow are extremely high temperatures and heat transfer to the wall of the spacecraft. At these temperatures, the assumption of thermal equilibrium is no longer valid and the effect of rotational non-equilibrium must be included in the modeling of diatomic gas flow. This …
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Dynamic nuclear polarization of amorphous and crystalline small molecules
… relaxation time is significantly reduced at low temperature (109 K), which negatively impacts DNP performance. A topical review of recent developments on high-field (16.4 T) DNP, as well as updates on the temperature-jump DNP experiment and descriptions of several ²H NMR studies of molecular …
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Thermo-mechanical response and plastic deformation behavior of Hastelloy X at elevated temperatures — role of dynamic strain aging
… strain aging is studied under isothermal and temperature jump experiments, using the technique of digital image correlation (DIC). The material of interest, a Ni-based superalloy, is widely used in high temperature applications, and it is important to understand its plastic response in such …
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Behavioral analysis and protein folding in zebrafish larvae
… mosaic transformation with fluorescence-detected temperature-jump microscopy to probe dynamics and stability of endogenously expressed proteins in different tissues of living zebrafish.
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2D IR spectroscopy and computational modeling : application to protein folding and binding
… JR spectroscopy of ubiquitin unfolding after a temperature jump. The emerging paradigm is to interpret 2D IR spectra with the aid of an atomistic, molecular dynamics simulation. The applications to protein binding use the monomer-dimer transition of insulin as a model system. Using a combination …
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In vitro and in vivo protein folding under stress
… in biological organisms, including pressure and temperature, which are the easiest stresses to simulate by molecular dynamics simulations. The thesis will focus on discussing the effect of pressure and thermal stress on proteins including some of the fast-folding model proteins, who’s in vitro …
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Mass Spectrometry for Determination of Conformation and Dynamics of Proteins and Structure and Biosynthesis of Bacterial Peptidoglycan
… Perturbations in protein structure are by temperature jump of the protein solution, followed by fast photochemical oxidation of proteins: FPOP) as the probe. The hydroxyl radical lifetime was predicted by a dosimeter experiment: Chapter 2). The T jump-induced folding constant was measured …
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Membrane and membrane protein dynamics studied with time-resolved infrared spectroscopy
… proteins are probed further by utilizing a rapid temperature-jump to induce a phase transition in the membrane. The response of the membrane, and membrane protein, to this phase transition reveals a picture of conformational change in a membrane protein slaved to the dynamics of the membrane.
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Understanding and Tuning Nanostructured Materials for Chemical Energy Conversion
… batteries. Rapid heating diagnostics tools, i.e. temperature-jump techniques, have been used to study the ignition of aluminum nanoparticles, nanothermite reaction mechanism and metal oxides nanoparticles decomposition under rapid heating conditions (~10<super>5</super>-10<super>6</super> K/s). …
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