Global ETD Search
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Showing 1 to 20 of 31 for “"telomeric DNA"”.
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Effect of oxidative stress on human telomeric DNA structural dynamics and accessibility
The ends of human telomeric DNA consist of single-stranded overhangs of tandem TTAGGG repeats (ssTEL) that can form into a G-quadruplex (GQ). This guanine-rich sequence is prone to 8-oxoguanine (8OG) damage, which is one of the most common oxidative DNA lesions that can compromise telomere …
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Interactions Between Platinum-Intercalator Hybrid-Agents and The Human Telomeric G-Quadruplex
Telomeres are stretches of non-coding DNA sequences in complex with multifunctional proteins, which form the termini of the chromosomes in most eukaryotic organisms. Two important roles of the telomeres are to prevent chromosome end-to-end fusion and loss of genetic information. While the gradual …
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Characterization of DNA Binding Activities at Vertebrate Telomeres
… by protein factors that either bind directly to telomeric DNA or that are recruited there by DNA binding activities. Thus, telomeric DNA binding activities are central to telomere function. Two types of vertebrate DNA binding activities are characterized in this thesis: proteins that can …
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Antigenic Variation and Telomere Structure in Trypanosoma brucei
… effect. Telomeres typically contain simple DNA repeats (TTAGGG in animals and trypanosomatids) that are the binding sites for telomeric proteins, and end in 3' protrusions known as overhangs. In mammals, telomeres fold back in structures known as t-loops, in which the overhang is …
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Biochemical analysis of proteins in the telomere maintenance pathway
… structures designated telomeres. The terminal telomeric DNA consists of tandem repeats of a G-rich motif that is established and maintained by the action of the specialized reverse transcriptase called telomerase. In addition to the function of telomerase, the telomere environment requires an …
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Telomere Position Effect in Human Cells
Telomeres are tracts of repetitive DNA that cap the ends of linear chromosomes. Each time the chromosome is duplicated, a small amount of telomeric DNA is lost from the end due to factors inherent in the mechanism of DNA replication. The result is a net shortening of telomeres with each cell …
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Interactions of small molecules with duplex DNA and lesion containing G-quadruplex DNA
… to study their interactions with duplex DNA. The nickel complexes were found to selectively oxidize guanines in bulged DNA structures in the presence of oxidant and notably NiCR-2H oxidizes guanines more efficiently than NiCR. According to 1 H NMR studies, NiCR-2H binds strongly to the N7 …
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Functional Analysis of the Human Telomeric Protein TRF2
… Binding Factor 2 (TRF2) is a ubiquitous human telomeric protein present at all telomeres throughout the cell cycle. TRF2 has been proposed remodel telomeres into large lasso-like structures named t-loops. Removal of TRF2 from telomeres by expression of a dominant negative allele of TRF2 …
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Further Insight into the Two Functions of Telomeres: Determining the Role of Tankyrase1 in Telomere Length Regulation and Tin2 in Telomere Protection
… cells. TIN2 interacts with the double-stranded telomeric DNA-binding proteins TRF1 and TRF2 independently or simultaneously, acting as a bridge linking TRF1 and TRF2 to TPP1 and POT1, the single-stranded telomeric DNA-binding protein. To gain further insight into the function of the TRF2-TIN2 …
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The Role of Pot1 in Telomere Protection and Maintenance
<p>POT1 is a single stranded telomeric DNA binding protein implicated in telomere length regulation in human cells. To address the role of POT1 in telomere protection we used RNAi in human cells. We confirmed that POT1 acts as a negative regulator of telomerase and showed that POT1 is required to …
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Telomerase : a tumor marker in the peripheral blood of colorectal cancer patients
… of a treatment. Telomerase is an RNA-dependant DNA polymerase that synthesises telomeric DNA sequences on the tip of chromosomes and is widely considered to be one of the most abundant and common tumour markers. Telomerase consists of two components; one is the functional RNA (hTR), which acts …
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Biochemical and structural studies of human telomerase regulation
… is a ribonucleoprotein (RNP) enzyme that extends telomeric repeats at eukaryotic chromosome ends (Blackburn and Collins, 2011; Blackburn et al., 2006; Greider et al., 1985; Morin, 1989; Nguyen, 2021). This counterbalances telomere loss caused by incomplete genome replication (Blackburn and …
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NOVEL G-QUADRUPLEX BINDERS WITH POTENTIAL FOR A DUAL DNA CROSS-LINKING MECHANISM OF ACTION
Genomic DNA is organized around the double-stranded of B-form DNA, which is both durable and flexible enough to store and pass on genetic information. Once freed from the associations of an extended complimentary sequence, single stranded DNA and RNA can adopt a vast array of stable secondary …
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NOVEL G-QUADRUPLEX BINDERS WITH POTENTIAL FOR A DUAL DNA CROSS-LINKING MECHANISM OF ACTION
Genomic DNA is organized around the double-stranded of B-form DNA, which is both durable and flexible enough to store and pass on genetic information. Once freed from the associations of an extended complimentary sequence, single stranded DNA and RNA can adopt a vast array of stable secondary …
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Mechanisms of Telomere End Protection by TRF2
… and preventing telomeres from activating various DNA damage response (DDR) signaling and double-strand break repair pathways. The need to suppress these DDR pathways at telomeres is known as the "end protection problem." The TRF2 subunit of shelterin is responsible for suppressing the ATM DNA …
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G4-RESOLVASE 1 TIGHTLY BINDS AND CATALYTICALLY UNWINDS UNIMOLECULAR G-QUADRUPLEX STRUCTURES WITH IMPLICATIONS FOR GENE TRANSCRIPTION AND TELOMERE BIOLOGY
… four-stranded structures formed by guanine-rich DNA or RNA sequences in which the strands can be orientated in parallel, antiparallel, or mixed parallel/antiparallel orientations. It has been previously shown that the DHX36 gene product, G4-Resolvase1 (G4R1), binds parallel tetramolecular G4-DNA …
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Distinct functions of POT1 at telomeres.
… the end of the chromosome from sites of DNA damage and control cellular replicative potential. The loss of function of telomeres results in several biological consequences. First, dysfunctional telomeres elicit DNA damage responses and repair activities, which frequently induce …
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On the Second Telomere Maintenance Machine: Structure, Recruitment, and Regulation of the CST–Polα/primase C-strand Fill-in Complex
… that safeguard genome integrity. Human telomeric DNA consists of double-stranded (ds) 5'-TTAGGG-3' repeats terminating in a 3' single-stranded (ss) overhang of the G-rich strand.The sequence and structure of the telomeric DNA is recognized and bound by the six-subunit shelterin complex, …
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Synthesis and biophysical evaluation of thiazole orange derivatives as DNA binding ligands
<p>Guanine-rich telomeric DNA at the end of chromosomes can form a unique DNA tertiary structure - G-quadruplex, which is known to inhibit the binding of telomerase to telomeric regions in cancer cells and thus regulate unrestricted cancer cell growth. Hence, G-quadruplex DNA has recently become a …
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Functional analyses of hTel2 and TRF2 deltaB: Insights Into the Role of a New DNA Damage Pathway and Homologous Recombination in Mammalian Telomere Function
… influences telomere length in S. cerevisiae and DNA-damage signaling in C. elegans. We found that endogenous human Tel2 (hTel2) localized in a diffuse, granular patter to the nuclei of human cells where it was enriched in PML nuclear bodies, but telomeres. Surprisingly, hTel2 also localized to …
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