Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"telomere replication"”.
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Flap Endonuclease 1 ensures telomere replication and stability
Telomeres, protein-DNA structures that distinguish the end of a chromosome from a bona fide DNA double strand break, are integral to genomic stability. High fidelity replication of telomeres is indispensable for their stability. Telomere replication is a challenging task that is completed through …
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Characterization and consequences of Telomere replication gone awry
Telomeres, the ends of linear eukaryotic chromosomes, pose several unique challenges to genome stability. My thesis work is concerned with the difficulties the replication machinery encounters when attempting to replicate telomeres, and with possible consequences of defects in telomere replication. …
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Mechanistic Understanding of the Role of TRF1 in Telomere Replication
<p>Mammalian telomeres are hard to replicate, and the shelterin subunit TRF1 is important for facilitating the replication of telomeric DNA. Deletion of TRF1 results in several phenotypes that are thought to be associated with telomere replication stress, including ATR signaling, fork stalling, …
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The Apollo Nuclease Binds To TRF2 and Protects
… of enzymes, hSNM1B/Apollo, in complex with the telomere binding proteins TRF2/Rap1. Due to its low abundance at telomeres, we consider Apollo to be a telomere accessory factor, and not part of the core telomere protein complex, shelterin. Apollo localizes to telomeres by interacting directly …
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ONCOGENE-INDUCED ALTERED DNA REPLICATION DYNAMICS
… By performing a whole genome analysis of DNA replication dynamics occurring upon oncogene activation, I discovered that oncogene activation alters DNA replication by increasing replication fork speed and fork stalling, while decreasing the frequency of replication initiation. This is …
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Defining Multiple Steps in Human Telomere End Processing
Telomere overhangs are essential for chromosome end protection and telomerase extension, but how telomere overhangs are generated is unknown. Due to the classic end replication problem, leading DNA daughter strands are initially blunt while lagging daughters are shorter by at least the size of the …
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Telomere Length Studies in Human Cancer Cells
Telomeres consist of repetitive DNA sequences and their associated binding proteins, and serve to protect linear chromosome ends from being recognized as double stranded breaks in need of repair. The telomeres of most normal diploid cells shorten with every cell division until they reach a …
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Development of embryonic stem cells expressing endogenous levels of a fluorescent protein fused to the telomere binding protein TRF1
Telomeres are the repetitive DNA sequence and associated proteins found at the ends of linear chromosomes. They have a role in biological processes including meiosis and aging as well as implications in a number of genomic instability disorders and cancers. Telomeres maintain genomic stability by …
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Discovery and Identification of New DNA Photoproducts and Investigation of the Redox Mechanism of Human Apurinic/Apyrimidinic Endonuclease 1 (hApe1) Protein
… type of DNA photodamage that interferes with telomere replication and present a unique challenge to DNA repair. We also describe the characterization of a new photoproduct mCA*, whose deamination product has the same structure as the well-known TA* photoproduct. The structure proof was done …