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Showing 1 to 9 of 9 for “"telomere length regulation"”.
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Functional Analysis of the Yku Complex in Telomere Length Regulation
… It contributes to the maintenance of wild type telomere length and, moreover, has been implicated in the protection of the telomeres from end-to-end fusions. Telomere-bound Yku delocalizes from telomeric foci in response to DNA damage and accumulates at the sites of a DNA break. This thesis …
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Further Insight into the Two Functions of Telomeres: Determining the Role of Tankyrase1 in Telomere Length Regulation and Tin2 in Telomere Protection
… poly(ADP-ribose) polymerase that can localize to telomeres through its interaction with the shelterin component TRF1. Tankyrase1 poly(ADP-ribosyl)ates TRF1 in vitro, and its nuclear overexpression leads to loss of TRF1 and telomere elongation, suggesting that tankyrase1 is a positive regulator of …
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Molecular and Genetic Analysis of the Role of Ku in Silencing and Telomere Function in Saccharomyces Cerevisiae
The binding of Ku at the telomere is an integral part of maintaining the specialized chromatin structure required for genomic stability at the telomeres. To gain further insight into the signals and complexes involved in the multiple processes contributing to normal telomere function, we performed …
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Distinct Functions of POT1 in Telomere Protection and Length Regulation
<p>Telomeres are DNA-protein structures that protect eukaryotic chromosome ends from illegitimate recombination and degradation. Telomeres become shortened with each cell division unless telomerase, a reverse transcriptase, is activated. In addition to playing a protective role of chromosome ends, …
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Identification and Characterization of the DNA Damage Response Function of Human Rif1 and a Search For Human Rap1 Interacting Factors
… of the yeast Rapl interacting factor 1, a telomere length regulator was identified. Immunofluorescence did not reveal an association of human Rifl with telomeres. Preliminary co-immunoprecipitation with known telomeric proteins and chromatin immunoprecipitation failed to show an interaction …
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The Role of Pot1 in Telomere Protection and Maintenance
… telomeric DNA binding protein implicated in telomere length regulation in human cells. To address the role of POT1 in telomere protection we used RNAi in human cells. We confirmed that POT1 acts as a negative regulator of telomerase and showed that POT1 is required to protect telomeres. …
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Biogenesis and Redox Transition of Iron-sulfur Clusters in Proteins
… example is Rtel1, a DNA helicase that regulates telomere length. Rtel1 was predicted to contain an iron-sulfur cluster, but this was not demonstrated. The third part of this work shows that the N-terminal domain of Rtel1 indeed contains a redox active [4Fe-4S] cluster. The second example is the …
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Mechanisms of Telomere End Protection by TRF2
… the ends of mammalian chromosomes, called telomeres. The six subunits of shelterin, TRF1, TRF2, TIN2, TPP1, POT1, and Rap1, each have distinct roles in telomere homeostasis. These include telomere length regulation and preventing telomeres from activating various DNA damage response (DDR) …
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On the Second Telomere Maintenance Machine: Structure, Recruitment, and Regulation of the CST–Polα/primase C-strand Fill-in Complex
<p>Telomeres are the terminal nucleo protein components of eukaryotic chromosomes that safeguard genome integrity. Human telomeric DNA consists of double-stranded (ds) 5'-TTAGGG-3' repeats terminating in a 3' single-stranded (ss) overhang of the G-rich strand.The sequence and structure of the …