Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"telomere biology"”.
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Oncogenic Kras and Telomere Biology In Crc Progression
… introduced modification enables us to replicate telomere-based crisis and genome instability, subsequently followed by telomerase reactivation. In prior studies employing telomerase-inducible mouse models of prostate cancer, the introduction of crisis-telomerase sequences led to the development …
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G4-RESOLVASE 1 TIGHTLY BINDS AND CATALYTICALLY UNWINDS UNIMOLECULAR G-QUADRUPLEX STRUCTURES WITH IMPLICATIONS FOR GENE TRANSCRIPTION AND TELOMERE BIOLOGY
… replication, immunoglobin switching, and telomere biology.
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A Critical Review of Telomerase Biology and Model Systems for the Study of Telomerase
The study of telomere and telomerase biology holds substantial promise in uncovering the molecular process of aging and the treatment of cancers. Studies have shown that telomere shortening is directly linked to cellular aging and that telomerase expression is found in over 85% of human cancers, …
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On the Second Telomere Maintenance Machine: Structure, Recruitment, and Regulation of the CST–Polα/primase C-strand Fill-in Complex
<p>Telomeres are the terminal nucleo protein components of eukaryotic chromosomes that safeguard genome integrity. Human telomeric DNA consists of double-stranded (ds) 5'-TTAGGG-3' repeats terminating in a 3' single-stranded (ss) overhang of the G-rich strand.The sequence and structure of the …
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Identification and Characterization of the DNA Damage Response Function of Human Rif1 and a Search For Human Rap1 Interacting Factors
… of the yeast Rapl interacting factor 1, a telomere length regulator was identified. Immunofluorescence did not reveal an association of human Rifl with telomeres. Preliminary co-immunoprecipitation with known telomeric proteins and chromatin immunoprecipitation failed to show an interaction …
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The Role of Pot1 in Telomere Protection and Maintenance
… telomeric DNA binding protein implicated in telomere length regulation in human cells. To address the role of POT1 in telomere protection we used RNAi in human cells. We confirmed that POT1 acts as a negative regulator of telomerase and showed that POT1 is required to protect telomeres. …
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A Novel Phosphorylation Site in the Telomeric Protein TRF2 is Regulated by the ATR Kinase and Plays a Role in Relieving Replication Stress at the Telomere
… (PIKKs) have a well documented function at yeast telomeres. Although several lines of evidence suggest that members of the PIKK family also play a role in vertebrate telomere biology, little is known about their specific functions. We report that the human shelterin component, TRF2, overexpressed …
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Telomere Dynamics and End Processing in Mammalian Cells
Telomeres are repetitive DNA sequences that end in single-stranded 3' overhangs. With each cell division, normal human cells lose a small amount of telomeric DNA due to the end-replication problem and the action of an unidentified nuclease. In order for tumor cells to divide indefinitely, they …
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Unravelling TERRA regulation by the interplay of two mechanisms: RALY interaction and telomere-specific polyadenylation
… at chromosome ends which play key roles in telomere maintenance. TERRA transcripts are of variable length and sequence that is characterized by a subtelomere-specific portion at the 5’ end and a common portion with telomeric repeats at the 3’ end. TERRA is implicated in different aspects of …
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Testing the T-loop Model of Telomeric End Protection
<p>Telomeres are the key structures that protect the ends of linear chromosomes. Although they are often thought about in the context of cellular aging, their most important role is actually to protect the end of the DNA from being mis-interpreted as a site of DNA damage. Telomeres are thought to …
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Exploring genetic interactions with G-quadruplex structures
… in transcription, replication, translation and telomere biology. Aberrant G4 formation and stabilisation have been linked to genome instability, cancer and other diseases. However, the specific genes and pathways involved are largely unknown, and the work within this thesis aims to investigate …
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Development of a T-Loop Assay to Investigate T-Loop Dynamics and Structure
… cancer therapy development has been the study of telomeres, which are repetitive sequences at the ends of chromosomes critical in maintaining genomic stability. T-loops, formed by the 3’ overhang inserting into the double strand region of the telomere, are thought to protect the ends from being …
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DIETARY BIOACTIVES IN THE MODULATION OF METABOLIC, FUNCTIONAL AND AGING BIOMARKERS
… and inflammation, intestinal permeability, and telomere integrity. The research was structured into three main chapters, combining both in vitro and in vivo approaches. In Chapter 1, the mechanisms of action of selected bioactives, including (poly)phenols and xanthine-derived metabolites, were …
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Mechanisms of Telomere End Protection by TRF2
… the ends of mammalian chromosomes, called telomeres. The six subunits of shelterin, TRF1, TRF2, TIN2, TPP1, POT1, and Rap1, each have distinct roles in telomere homeostasis. These include telomere length regulation and preventing telomeres from activating various DNA damage response (DDR) …
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Clarifying the Role of the CST Complex in DNA Replication and Repair
… maintained by specialized structures known as telomeres. These structures are protected by a number of essential protein complexes including the shelterin complex and CST (CTC1 – STN1 – TEN1) complex. CST is an RPA-like ssDNA binding protein that is vital for telomere length maintenance via …
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RNA-MEDIATED PHASE SEPARATION OF 53BP1: CONNECTING CONDENSATE BIOPHYSICS TO DNA DAMAGE RESPONSE
… non-homologous end joining (NHEJ) of deprotected telomeres, as measured by the frequency of telomere fusions, highlighting its functional relevance in DNA repair. Finally, we prove that antisense oligonucleotides (ASOs) targeting telomeric transcripts reduce protein-RNA binding affinity, providing …