Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 217 for “"target gene"”.
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TRIM22/Staf50 - a novel target gene of the tumor suppressor p53
The tumor suppressor gene p53 is a transcription factor that mediates cell cycle arrest, apoptosis and differentiation. p53 mediated differentiation of leukemic cells depends on the transcriptional activity of p53 but so far no obvious differentiation inducing target gene has been identified. Using …
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On the function of the interferon-inducible p53 target gene TRIM22
The interferon-inducible p53-target gene TRIM22 (Staf50) is a member of the TRIM (TRIpartite Motif) family. The TRIM family is a family of RING (Really Interesting New Gene) proteins, defined through a tripartite motif, containing a RING-domain, one or two B-boxes and a Coiled-coil-domain. The TRIM …
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Mechanism of Human Papillomavirus E6-Mediated Repression of P53-Target Gene *Transcription
… human p21 WAF1 cyclin-dependent kinase inhibitor gene transcription, independent of its well known role to promote the ubiquitination and degradation of p53. Using both high-risk and low-risk HPV E6 oncoproteins, each representing a class of oncoproteins that is capable of inducing p53 degradation …
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PAX3 expression, protein modifications and downstream target gene profiling in melanocytes and melanoma cells
… embryo. As a result of alternative splicing, the gene gives rise to eight different transcripts which encode proteins that have differing structures and are therefore likely to activate different downstream target genes. The presence of post-translational modifications has also been shown to alter …
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A regulatory role for repeated decoy transcription factor binding sites in target gene expression
… in the number of TRs present within or close to genes is known to lead to gross phenotypic changes and disease. However, whether intergenic TRs have a functional role is less understood. Recent studies reveal that many intergenic TRs contain transcription factor (TF) binding sites and that …
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Using the Cre-loxP system to randomize target gene expression states and generate diverse phenotypes
Modifying the expression of multiple genes enables both deeper understanding of their function and the engineering of complex multigenic cellular phenotypes. However, deletion or overexpression of multiple genes is typically laborious and involves multiple sequential genetic modifications. Here we …
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The Arabidopsis C/S1 bacic leucine Zipper transcription factor network:Impact of heterodimer formation on target gene transcription
… eine wichtige Rolle bei der Regulierung der Genexpression. Zur Analyse der Heterodimerisierung der Arabidopsis thaliana (At) bZIP-Faktoren wurde ein Gateway® basierendes two-hybrid System in pflanzlichen Protoplasten etabliert und mit den Daten aus dem klassischen Hefe two-hybrid (Y2H) …
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Mechanistic Insights into the Notch signalling pathway through Molecular Condensation
… signalling lead to dynamic changes in Notch target gene expression. One potential mechanism that has recently been shown to play a role in titratable gene expression of several signalling pathways is the ability for proteins to undergo molecular condensation within living cells. To date, …
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β-Globin Enhancers Regulate Bistable Gene Expression States
Multiple enhancers can coordinately regulate a target gene to ensure robust expression. Under the failure rate model, each redundant enhancer contributes a probability of target gene expression in the cell, and genetic deletion will cause single-cell expression phenotypes. However, this model has …
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Odd-paired: a zinc finger pair-rule protein required for the timely activation of engrailed and wingless in Drosophila embryos
<p>Here I report a molecular and genetic analysis of the pair-rule gene odd-paired (opa). Genetically, opa is essential for the appropriate level and timing of en and wg expression in all parasegments, but opa activity alone does not determine the restricted spatial domains of these genes. I …
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MODELING FACIOSCAPULOHUMERAL MUSCULAR DYSTROPHY USING PRIMARY AND PATIENT-DERIVED INDUCED PLURIPOTENT STEM CELLS
… Dystrophy (FSHD) is an autosomal dominant degenerative muscle disease with no cure or treatment. The genetic cause of FSHD is the reduction of the copy number of subtelomeric D4Z4 repeats at 4q35 encoding Double Homeobox 4 (DUX4) protein, which is a potent transcription factor that is toxic …
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Investigating the role of Notch signalling during mesoderm development and cell proliferation
… it also acts as its own effector and regulates target gene expression. This unique characteristic renders cells and tissues sensitive to varying levels of Notch activation. Although widely studied, the role of Notch signalling during mesodermal specification remains unclear. In addition, novel …
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Investigation of ARNT isoform-specific regulation of AHR signaling
… whereas ARNT isoform 3 is needed for AHR target-gene transcription. Furthermore, to explore this suppressive role, we investigated a unique modification specific only to ARNT isoform 1 – phosphorylation of serine 77 (S77). We determined that phosphorylation at S77 is initiated following …
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Analysis of combinatorial gene regulation with thermodynamic models
… of TFs bound, the expression level of a target gene is usually determined by the combinatorial control of a number of TFs. The interactions among regulatory proteins and their regulatory sequences collectively form a regulatory network. A major challenge in the study of gene regulation is …
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Spatio-temporal investigation and quantitative analysis of the BMP signaling pathway
Bone Morphogenetic Proteins (BMPs) are key regulators for a lot of diverse cellular processes. During embryonic development these proteins act as morphogens and play a crucial role particularly in organogenesis. BMPs have a direct impact on distinct cellular fates by means of …
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A Role for the Ecdysone Response Gene E93 in Imaginaling Patterning during Metamorphosis
… melanogaster E93 is an early ecdysone response gene that encodes a pipsqueak domain transcription factor. E93 is induced by ecdysone at the end of larval development, and directs the death and elimination of several larval tissues during metamorphosis. Although E93 has been considered a …
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Modulating Bone morphogenetic protein (BMP) and Transforming growth factor-beta (TGF-β) signalling with green tea catechins in mammalian cells
… a loss of characteristic mutation in the BMPR2 gene, a member of the TGFβ Superfamily. In this study, it was hypothesised that these green tea catechin compounds might inhibit TGFβ signalling while promoting BMP signalling. This study demonstrates that these green tea catechin compounds inhibit …
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Characterization of Cis-Regulatory Elements Controlling Repo Transcription in Drosophila Melanogaster
The glial cells missing (gcm) gene has been identified as a "master regulator" of glial cell fate in the fruit fly Drosophila . However, the gcm gene is also expressed in and required for the development of larval macrophages and tendon cells, and lamina neurons in the adult CNS. Thus, the Gcm …
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The Effects of Gain of Function Mutant p53 and p63 on EPS8 and CXCL5 Expression in Head and Neck Squamous Cell Carcinoma
… functions that have been linked to tumorigenesis. In this current study, we utilized a model system consisting of cells derived from HNSCC tumors in order to investigate our hypothesis that GOF mutant p53 enhances, and p63 inhibits, EPS8 and CXCL5 expression and promoter activity. We found …
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Discovering the p53 isoforms’ code
p53 has a key role in the maintenance of the genetic stability and, thus, in preventing tumour development. The current model is that the p53-dependent responses are solely driven by p53α. The uncovering that the TP53 gene physiologically expresses several p53 isoforms, challenges this paradigm. …
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