Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 22 for “"target discovery"”.
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OPTIMISATION AND THERAPEUTIC-TARGET-DISCOVERY ORIENTED ANALYSIS OF CRISPR-CAS9 SCREENS
… has revolutionized cancer research by enabling targeted genome modifications in tumour cells, offering insights into cancer biology and potential treatment targets. My PhD research focuses on developing tailored tools and pipelines to enhance the analysis of CRISPR-Cas9 screens for identifying …
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High-dimensional machine learning for drug target discovery and precision medicine
… or cancer is essential to discover molecular targets for therapeutic intervention, ideally in a personalized manner. Gene expression measured in a tissue sample is an informative proxy to the protein levels in that tissue and known to drive disease outcomes. Machine learning offers promising …
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A Context-Forward In Vivo Functional Genomics Platform For Target Discovery and Establishing Vulnerability Context In Pancreatic Cancer
… is required to establish relevant context for targeted therapy. To this end, we sought to establish an integrated platform to: i) more comprehensively characterize differential oncogenic signaling across our tumor models, and ii) establish a disease-specific co-expression network to delineate …
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CRISPR AS A TOOL FOR TARGET DISCOVERY AND TECHNOLOGICAL INNOVATION: HIGH-THROUGHPUT CBE SCREENING TO EVALUATE M6A MODIFICATION PATHWAY IN CANCER PROLIFERATION AND DRUG RESISTANCE, AND INNOVATIVE PRIME EDITING STRATEGIES FOR DISEASE MODELLING AND THERAPY
… by “readers”, which mediate in the regulation of target RNAs, for instance by promoting splicing, nuclear export, stability and decay. Altered m6A deposition, removal and identification affect gene expression resulting in pathogenic disease phenotypes such as cancer proliferation. For instance, …
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The G protein-coupled receptor GPR30 signalosome - A novel G protein-independent mechanism regulating cAMP signaling and receptor trafficking
… current market are either directly or indirectly targeted towards the function of GPCRs, we can be certain of their considerable importance. This thesis is dedicated solely to one particular GPCR, GPR30. This receptor is shrouded in uncertainty with contradictory results and opposing views on …
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Machine learning models for functional genomics and therapeutic design
… leverage such knowledge to provide insight for target discovery and therapeutic design.
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In-vitro analysis of microfilariae phenotypic responses induced by small molecules and dsRNA targeting receptor pathways
… However, with resistance on the rise, drug target discovery and development are urgently needed. This study explores phenotypic responses in microfilariae to small molecules as well as dsRNA molecules targeting genes involved in receptor pathways essential for nematode function. Utilizing …
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Characterizing Failure Modes in Continuous Flow Microfluidic Pumps
… novel platforms for drug testing and therapeutic target discovery, through their three-dimensional morphologies that mimic specific tissues and organs. In concurrence with these systems, microfluidic channels allow for a sufficient supply of nutrients to the cell culture; by creating pressure …
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BIOINFORMATICS TOOL AND MODEL DEVELOPMENT FOR STUDYING BIOLOGICAL NETWORKS AND PROTEIN-PROTEIN INTERACTIONS
… have transcended the global network in biomarker/target discovery, we developed TISPIN database for human TIssue-Specific Protein Interaction Networks, with improved features over the existing resources. PPIs have been explored as therapeutic targeting space, where the drug potency is highly …
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In Vivo Gene Expression Profiling of the Plague Bacillus, Yersinia Pestis
… data suggest these regions may provide promising targets for future therapy design and vaccine target discovery.
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Post-genomic studies on the neuropeptide signalling system of Juvenile <i>Fasciola hepatica</i>
… sensitive fluorescent version. Overall, through targeting 45 transcripts using RNAi among other methods, this project has revealed the complexity and importance of the neuropeptide signalling pathway in liver fluke and provides a foundation for more focussed studies on drug target discovery and …
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Microdialysis to study the Gastrin-ECL cell Axis in the conscious rat
The ECL cells constitute the major endocrine cell population in the acid-producing part of the stomach. Gastrin from G cells in the antrum is the main stimulus of gastric acid secretion. Gastrin stimulates the ECL cells in the oxyntic mucosa to mobilize histamine, which in turn stimulates the …
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Computational Approaches to Delineate Transcriptional and Functional Heterogeneity In Pancreatic Cancer
… computational methodologies to aid in refined target discovery, and to develop a novel platform to comprehensively characterize the transcriptional heterogeneity of PDAC. Specifically, focusing on a large PDAC PDX cohort, we focused on a) establishing a PDAC co-expression network to serve as a …
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Omics analysis in understanding disease mechanisms
… of omics technologies has revolutionized drug target discovery, transitioning from phenotypic and function-centric methodologies to more mechanistic and target-driven approaches. To maximise drug development success at earlier phases, understanding the role of targets in disease mechanisms is …
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Integrating Functional Genomics with Systems Biology to Discover Drivers and Therapeutic Targets of Human Malignancies
… studies that may lead to therapeutic target discovery for human malignancies in the era of personalized medicine. However, due to high false-positive and false-negative rates arising from noise of high-throughput measurements and off-target effects, powerful computational tools and …
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Determining therapeutically actionable genetic interactions in human cancer at scale using multiplexed CRISPR screening
… in precision oncology, enabling the selective targeting of gene loss in cancer. This study employed a high-throughput multiplexed combinatorial CRISPR/Cas9 pooled screening approach to systematically identify SL interactions. A novel tRNA-based dual guide expression system was used to construct …
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Advancing T Cell–Based Immunotherapies: Identification of Tumor-Specific T Cell Receptor Targets and Mechanisms of Resistance in Solid Tumors
… efficacy is partly due to the absence of optimal target antigens and the development of tumor-intrinsic immune resistance mechanisms that enable evasion of immune surveillance. Overcoming these challenges will be critical to enhance the effectiveness of cancer immunotherapies and improve patient …
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Novel Statistical Methods for Mediation Analysis with High-dimensional Omics Mediators
… insights for precision medicine and therapeutic target discovery.</p>
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Investigating the protein targets of the neuroprotective E3 ligase, CHIP
… events and provide promising therapeutic targets for disease modification. A key player in the regulation of proteostasis is the dual function chaperone and E3 ligase C-terminus of Hsc-70 interacting protein (CHIP). Its role in neurodegenerative diseases, including dementia with Lewy …
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