Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"tamoxifen-inducible"”.
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Syntaxin-3 mediates baseline and stimulated mucin secretion
… Stx mutant/floxed mice to Scgb1a1tm1(icre)fjd or tamoxifen-inducible Scgb1a1tm1(cre/ERT)blh/J mice. To measure mucin secretion defects, intracellular mucin was stained with Periodic Acid Fluorescence Schiff’s reagent (PAFS) and quantified with image analysis. In uninflamed mice, the baseline rates …
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Mechanically driven In vivo Osteocyte Ca2+ Signaling: Aging and Pharmacologic Effects
… GCaMP signal throughout its lifespan. We used a tamoxifen-inducible GCaMP reporter mouse that only expressed GCaMP signal when needed. This tamoxifen-inducible mouse has been used in other bone research studies in mice up to 18 months of age. In Chapter 3, we compared the in vivo osteocyte Ca2+ …
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Uncovering astrocyte roles at the blood brain barrier in the healthy and concussed brain
… small number of astrocytes using a conditional, tamoxifen-inducible mouse model. Within 2 hours after induction, I observed sparse astrocyte death in the cortex and leakage of the small molecule Cadaverine and large plasma protein fibrinogen, which are normally contained by a functional BBB. …
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RDH10 is Necessary for Initial Vagal Neural Crest Cell Contribution to Embryonic Gastrointestinal Tract Development
… for RA in ENS development between E7.5-E9.5. Tamoxifen-inducible temporal deletion of RDH10 at E6.5-E7.5 confirms this early retinal role, while later RDH10 deletion suggests retinal independence for continued enteric NCC colonization. Removing RDH10 from NCCs shows no gross or ENS-specific …
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Determining if the Developmental Origin of Cardiac Fibroblasts Determines the Fate of Activation in Adult-Onset Hypertension
… test this, we used genetic lineage tracing via a tamoxifen-inducible Cre model to label Periostin+ fibroblasts in neonatal mice during the first 9 days in the postnatal period, before challenging them with AngII/PE in adulthood to determine if they reactivate upon injury. Histological analysis of …
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Mash1 Defines Lineage Restricted Neuronal and Oligodendrocytic Precursor Cells in Spinal Cord Development
… generated that express GFP, Cre recombinase, and tamoxifen-inducible Cre recombinase. The M1-GIC mouse line, showed faithful Mash1 expression recapitulation and traces Mash1+ progenitors mainly to dI3 and dI5 interneurons. This supports data from the Mash1 null mutant where these populations are …
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Removal of Pax6 from cortical progenitors renders them abnormally likely to switch fate in response to Sonic Hedgehog activity
… Pax6-floxed (Pax6fl/fl) mutant mice expressing a tamoxifen-inducible form of Cre recombinase under the control of the Emx1 locus (Emx1-CreERT2) and an EGFP reporter construct. Cultured cortical progenitors were exposed to increasing concentrations of Shh agonist, developed for the protein …
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Role of SH3 and Cysteine-Rich Domain 3 (STAC3) in Skeletal Muscle Development, Postnatal Growth and Contraction
… in postnatal mice through the Flp-FRT and tamoxifen-inducible Cre-loxP systems. Postnatal Stac3 deletion inhibited body and limb muscle mass gains. Histological staining and gene expression analyses revealed that postnatal Stac3 deletion decreased the size of myofibers and increased the …
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Malignant Gliomas Originate From Neural Stem/Progenitor Cells and Are Maintained By Cancer Stem Cells
… of NSCs in glioma, we developed an NSC-specific tamoxifen-inducible nestin-cre driver mouse. When crossed to the NF/p53/Pten flox mice and induced with tamoxifen at E13.5, the Nes-Cre;Nf/p53/Pten mutant mice exhibited tumor initiation and progression similar to our previous mouse model using …
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Spatial and temporal regulation of cerebral cortex development by the transcription factor pax6
… mid- or late-stages of corticogenesis by using a tamoxifen-inducible Emx1-CreER line. I report a novel requirement of Pax6 for continuous suppression of ventral fates and concurrent maintenance of an appropriate dorsal identity in cortical progenitors. Pax6 ablation at either mid- or late-stages …
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Investigating the Mechanisms Underlying the Heterogeneous VSMC Contribution to Vascular Disease
… of colours at any stage of plaque development. Tamoxifen-inducible, VSMC-specific deletion of contractile master regulator Myocd in adult mice had modest phenotypic effects on baseline VSMC contractile marker expression, and on injury-induced VSMC transcriptional response and clonality. In both …
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Mechanism of CASK-linked ophthalmological disorders
… CASK neuronal knockout (CASKNKO) and tamoxifen inducible CASK knockout (CASKiKO) mouse models to investigate the mechanism and pathology of CASK-linked ONH. Our observations indicate that ONH occurs with 100% penetrance in CASK(+/-) mice, which also displayed microcephaly and …
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Endosomal membrane dynamics underlying cell spreading: A role for the small GTPase Arf6
… for the acute deletion of Arf6 using a tamoxifen inducible Cre/loxP recombination system. Acute deletion of Arf6 in these MEFs resulted in a kinetic delay in transferrin recycling as well as in cell spreading. The spreading delay correlated with reduced trafficking of cholera toxin …
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The temporal requirement of IL-4Rα signalling in allergic asthma and the role of IL-4Rα-responsive Regulatory T cells in restraining allergic airway inflammation
… We used RosacreERT2IL-4Rα-/lox mice, a novel, tamoxifen inducible IL-4Rα knockdown model to investigate the role of IL-4/IL-13 signalling during the effector phase of ovalbumin induced allergic airway disease (AAD) and for the onset of the disease. The deletion of the IL-4Rα had a therapeutic …
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Molecular biology of peripheral nerve regeneration: Expression and role of Netrin-1, DCC and Runx2
… a global knockout of DCC in adult mice using a Tamoxifen inducible Cre recombinase system. A transection injury in DCC global knockout mice revealed aberrant trajectory of axons in the nerve bridge, however innervation of the distal nerve was not affected. Similar to the Schwann cell-specific …
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The role of endothelin-2 in ovulation
… ablate Ednra in smooth muscle cells through a tamoxifen-inducible Cre recombinase system. However, all mice including wild type controls treated with tamoxifen had severely impaired ovulation; while ovulation occurred, the role of EDNRA cannot yet be determined. Alternatively, available data …
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Analysis of the role of the mineralocorticoid receptor in renal principal cell sodium reabsorption in vivo
… postnatal lethality of the MRAQP2Cre mice, an inducible principal cell-specific gene inactivation system was established in parallel to induce MR inactivation in adult mice. The large genomic DNA fragment harbouring the AQP2 gene, used for the generation of the constitutive AQP2Cre transgene, …
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Investigating the role of microglial senescence in central nervous system injury and regeneration
… in microglia specifically using a Tmem119 tamoxifen-inducible Cre line crossed to a model with floxed Mdm2, a negative regulator of senescence. Despite numerous rounds of troubleshooting, microglial senescence was not induced in this model. Therefore, I performed an alternative functional …
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Non-canonical functions of leucyl-tRNA synthetase: Mechanism of cell growth and skeletal myogenesis
… I propose an alternative strategy using tamoxifen-inducible Myf5-CreER driven Vps34 knockout in muscle cells of the offspring to investigate the role of Vps34 in the early stage of muscle regeneration. Additionally, I suggest an experiment to probe the in vivo involvement of the …
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A Characterisation and Understanding of the Germinal Centre B Cell Response to Atherosclerosis in Mice using Lineage Tracing
… dysregulated in atherosclerosis. The tamoxifen-inducible AID-CreERT2-Rosa-EYFP lineage tracing mouse model was used and crossed with the atherosclerosis-prone LDLr-deficient mouse model. This model inducibly and specifically labels germinal centre B cells and their progeny using …
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