Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 42 for “"tRNA synthetases"”.
-
tRNA synthetases as potential RNA capping enzymes
… pro enzym vytvářející NpnN-RNA jsou aminoacyl-tRNA syntetázy. Tyto enzymy jsou, kromě své esenciální role v translaci, odpovědny za syntézu volných dinukleosid polyfosfátů v buňce. V této práci jsme vybrali čtyři tRNA syntetázy, klonovali je do plazmidů a purifikovali pomocí proteinové …
-
The Hydroxamate Reaction of Aminoacyl-tRNA Synthetases
… which remain bound to the aminoacyl-tRNA synthetases that catalyze their formation. The activated amino acids are then esterified to specific transfer RNA molecules. By this reaction sequence, the specificity and energetics necessary for polypeptide synthesis are conferred upon the …
-
BIOCHEMICAL ANALYSIS OF TWO DISTINCT METHIONYL-TRNA SYNTHETASES
Aminoacyl-tRNA synthetases (AARSs) are essential enzymes that catalyze the attachment of amino acids to their cognate tRNAs for protein biosynthesis at the ribosome. The long term objective of this project is to investigate catalytic features of methionyl-tRNA synthetase (MetRS) from two different …
-
The C-terminal domains of leucyl-tRNA synthetases
The mitochondrial Leucyl-tRNA synthetase (mtLeuRS) of certain Caenorhabditis species contains an idiosyncratic C-terminal addition. Bioinformatic analyses identified this domain in the mtLeuRS and also four putative nuclear localization sequences (NLSs) encoded within the domain (Ezak, Hong, …
-
Interactions Between Aminoacyl-Trna Synthetases and Transfer-Rnas From Escherichia Coli
Made available in DSpace on 2014-12-09T18:53:06Z (GMT). No. of bitstreams: 1 6901483.pdf: 1925547 bytes, checksum: 1341f16b04a547adb4610578659ace00 (MD5) Previous issue date: 1968
-
Regulated alternative splicing separates canonical and cell signaling functions of aminoacyl-tRNA synthetases
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-05-01
-
Characterization of the Fidelity Mechanisms of Leucyl -Trna Synthetases From Saccharomyces Cerevisiae and Escherichia Coli
… site for post-transfer editing of mischarged tRNA similar to other LeuRSs. However, unlike other origins, when LeuRS amino acid editing-defective mutants were introduced in vivo, cell viability was largely unaffected. In contrast, these editing-defective mutations limited viability of E. coli …
-
Localization of tRNAs and aminoacyl-tRNA synthetases in cytoplasm, chloroplast and mitochondria of Glycine max, L.
Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1980 .S553. Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1981.
-
Enzymatic and Splicing Activity of Class I Leucyl -Trna Synthetases From Escherichia Coli and Saccharomyces Cerevisiae
Leucyl-tRNA synthetase from yeast mitochondria is also required for splicing of the mitochondrial bI4 and al4a introns. Splicing of these introns is dependent on coordination between ymLeuRS and bI4 maturase. An in vitro splicing assay has been established that is based on assembly of proteins into …
-
FUNCTIONAL EFFECT OF ALTERATIONS TO E. coli METHIONYL-tRNA SYNTHETASE BETA-LINKER LENGTH
Aminoacyl-tRNA synthetases (AARSs) are essential enzymes that catalyze the
-
Evolution of Protein Structure
The aminoacyl-tRNA synthetases are one of the major protein components in the translation machinery. These essential proteins are found in all forms of life, and are responsible for charging their cognate tRNAs with the correct amino acid. The evolution of the tRNA synthetases is of fundamental …
-
Aminoacylation kinetics and specificity for viral genomic RNAs
The esterification of amino acids to the tRNA-like structures of viral genomic RNA was studied as catalyzed by amino acyl tRNA synthetases from E. coli, yeast, bean and rat. Peanut stunt virus RNA was esterified with tyrosine in the presence of bean tyrosyl-tRNA synthetase to a maximum of 22 mole …
-
Evaluation of the Protein Recognition Properties of Peptide Nucleic Acids
… is to evaluate the ability of aminoacyl-tRNA synthetases (aaRS) to recognize the non-standard nucleic acid, PNA (peptide nucleic acid). PNA has immense potential in biomedical applications due to its increased thermostability and nuclease resistance over natural nucleic acids. PNA …
-
Kinetic and mutational studies of two RNA-interacting enzymes
… disrupt RNA secondary structure, and aminoacyl-tRNA synthetases, which catalyze the attachment of an amino acid to its cognate tRNA.
-
Characterization of Molecular Structure-Function Relationships of Escherichia Coli Leucyl-Trna Synthetase
The accurate covalent linkage of amino acid to tRNA for protein synthesis is catalyzed by a family of aminoacyl-tRNA synthetases (aaRS). Some aaRSs have the potential to make mistakes during aminoacylation due to the nature of their amino acid substrate. However, the synthetases have …
-
Characterizing the landscape of aminoacyl-tRNA synthetase protein production in Bacillus subtilis
… Here I use a model enzyme family, the aminoacyl tRNA synthetases (aaRS), to explore how sensitive Bacillus subtilis are to changes in aaRS production from the molecular to phenotypic level. This culmination of protein levels, functional output, and fitness, leads to a complete "fitness landscape" …
-
Characterizing CMT-causing variants in tryptophanyl- tRNA synthetase
Aminoacyl-tRNA synthetases (aaRSs) are essential enzymes that link amino acids to their cognate tRNAs. Neurological conditions, such as Charcot-Marie-Tooth (CMT) disease, have been linked to variants identified in these enzymes. I created a humanized yeast model to assess the underlying …
-
The Invariant Arginine In Motif 2 of ESCHERICHIA COLI Alanyl-tRNA Synthetase : Is Important For Catalysis But Not For Substrate Binding
… located in the active site of class II aminoacyl-tRNA synthetases, each contain an invariant arginine thought to participate in interactions with ATP. For <em>Escherichia coli</em> alanyl-tRNA synthetase (AlaRS), sequence comparisons indicate that Arg69 should be aligned with the invariant …
-
Biochemical Analysis of the Molecular Factors of Leucyl-Trna Synthetase That Ensure Aminoacylation Fidelity
The tRNA synthetases catalyze aminoacylation of transfer RNA (tRNA) with specific amino acids. Accurate aminoacylation is critical to the fidelity of protein synthesis. Leucyl-tRNA synthetase (LeuRS) can misactivate a broad scope of non-leucine amino acids. In these cases, mischarged tRNA products …
-
Engineering exclusively-quadruplet codon translation in vivo
… the task of assembling enough quadruplet-tRNAs (qtRNAs) to implement an all-quadruplet code remains a major hurdle. Here, we create qtRNAs that decode canonical amino acids by modifying E. coli tRNAs that continue to rely upon endogenous aminoacyl-tRNA synthetases (AARSs) for charging. We …
Page 1 of 3