Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"tRNA gene"”.
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Development of a Yeast Transcription System Using a Saccharomyces Cerevisiae Enzyme Extract and a Drosophila Melanogaster Histidine tRNA Gene
… transfer ribonucleic acids. Plasmids containing tRNA genes were a gift from Dieter Soll, Yale University Department of Molecular Biophysics and Biochemistry. In order to provide increasing amounts of the cloned gene, the bacterium Escherichia coli HB101 was transformed using standard …
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Genetic and mechanistic analysis of heterochromatin spreading in the yeast S.cerevisiae
RNA Polymerase III transcribed tRNA genes are implicated in a wide variety of chromosome organizational functions that includes the ability to act as a boundary to heterochromatic silencing. A tRNA gene has been shown to be a major component of the barrier that prevents spreading of silencing from …
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Extra-Transcriptional Effects of Chromatin Bound RNA Polymerase III Transcription Complexes
… Pol III-specific transcription factors. At the tRNA gene, the TFIIIC complex recognizes and specifically binds at intragenic promoter elements A-box and B-box and aids the assembly of TFIIIB to upstream of the transcriptional start site. Upon binding, Pol III is recruited near start sites and …
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Non-Coding Genomics of Methanocaldococcus Jannaschii: A Survey of Promoters, Non-Coding RNA Genes, and Repetitive DNA Elements
… genomic components and are operative in genetic information remission, transfer, and processing---key processes where the three domains of life, Bacteria, Archaea, and Eucarya, differ from one another. This thesis focuses on some non-coding features of an archaeal genome, that of …
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Mechanismus des pre-tRNA-Spleißens : Struktur und Funktion pflanzlicher und animaler RNA-Ligasen
… werden von der RNA Polymerase III als Vorläufer tRNA transkribiert und durchlaufen eine Vielzahl von Reifungsschritten hin zur maturen tRNA. Neben der Hydrolyse der 5´- und 3´-Flanke durch die RNase P und die tRNase Z, sowie einer Vielzahl von Basenmodifizierungen, wird bei einigen pre-tRNAs das …
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Characterizing Human Transfer RNAs by Hydro-tRNAseq and PAR-CLIP
<p>The participation of tRNAs in fundamental aspects of biology and disease necessitates an accurate, experimentally confirmed annotation of tRNA genes, and curation of precursor and mature tRNA sequences. This has been challenging, mainly because RNA secondary structure and nucleotide …
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Gene Expression in Archaea: Studies of Transcriptional Promoters, Messenger RNA Processing, and Five Prime Untranslated Regions in Methanocaldococcus Jannashchii
Gene expression in Archaea is less understood than those in Bacteria and Eucarya. In general, three steps are involved in gene expression---transcription, RNA processing, and translation. To expand our knowledge of these processes in Archaea, I have studied transcriptional promoters, messenger RNA …
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Nucleotides in yeast phenylanyl - transfer-RNA required for the specific recognition by its cognate yeast phenylalanyl - transfer-RNA synthetase
A method for synthesizing yeast tRNA$\sp{\rm Phe}$ mutants having nucleotide substitutions at any desired position in the tRNA has been developed. Using synthetic DNA oligonucleotides, recombinant plasmids were constructed with a T7 promoter directly upstream of the tRNA gene and a BstN 1 …
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Studies on the architecture and on transferability of pathogenicity islands of uropathogenic Escherichia coli strain 536
… to gain deeper insight into bacterial pathogenesis. With the help of comparative genomics, microbiological research can focus on those DNA sequences that are present in pathogenic bacteria but are absent in non-pathogenic strains. With this knowledge and with the help of molecular biological …
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Intracellular Traits of Chimeric tRNAlys3-ribozymes and their Inhibition of HIV
<p>Host tRNALYs3is the primer recruited by HIV-1 for initiating reverse transcription. When ribozymes targeted upstream of the primer binding site of the HIV-1 genome are tethered to the 3' end of the tRNALYs3, it is expected that the tRNA will carry the ribozymes into the virions. We made …
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Characterization of the Integrase of the Bacteroides Mobilizable Transposon NBU1
… tyrosine recombinases. I used site-directed mutagenesis to construct alanine mutants of these six residues. I tested the mutant proteins using an E. coli conjugation assay and showed that the mutants were defective for in vivo integration. This confirmed that IntN1 is a tyrosine recombinase. NBU1 …
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Tracking translation factors in fission yeast nucleus
… the association of RPs occurs across specific genes/transcripts or whether chromatin association is genome wide. In agreement with previous studies in D. melanogaster and S. cerevisiae, I found that RPs preferentially associate to transcriptionally active genes. ChIP followed by analysis on …
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Untersuchungen zum Einfluß der Pathogenitätsinseln I536 und II536 auf die Genexpression des uropathogenen Escherichia coli Stammes 536
… werden und durch die sie sich von apathogenen Stämmen unterscheiden. Der uropathogene E. coli Stamm 536 (O6:K15:H31) exprimiert verschiedene Virulenzfaktoren wie a-Hämolysin, die Adhäsine S-, P-related (Prf) und Typ 1-Fimbrien sowie das Kapselantigen K15. Außerdem wurden auch …