Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"systematic evolution of ligands by exponential enrichment (SELEX)"”.
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Using Capillary Electrophoresis for High-Throughput Molecular Screening
Advances in the development of DNA-encoded chemical libraries have necessitated rapid selection strategies to aid in molecular discovery. Encoded chemical libraries are composed of thousands of unique (potential) drug molecules, each encoded with a corresponding unique DNA sequence, allowing for …
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Studying the Structural Dynamics and Aptamer-Ligand Interactions in the Cocaine-Binding Aptamer through Fluorescence Spectroscopy
… utilized in biosensor modeling technologies. One of the most important advantages of using aptamers is the structural flexibility and thermal stability of nucleic acids. These structural merits enable aptamers to be linked on solid surfaces, attached to chemical labels, or extended to build …
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In vitro selection of aptamers using quantum dot-assisted capillary electrophoresis SELEX
… bind to targets with high affinity. Selection of aptamers is accomplished by the Systematic Evolution of Ligands by EXponential enrichment (SELEX). The target is incubated with a random sequence oligonucleotide library, and target-bound sequences are separated from the unbound pool to generate …
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A computational approach for the discovery and characterization of oligonucleotide aptamers for protein targets
… bind with very high affinities to a broad range of chemical and biomolecular targets, including proteins, cells, viruses, microorganisms, toxins, and chemical compounds. They are analogous to antibodies with applications in therapeutic delivery, biosensors, and diagnostics, but possess the …
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Aptamers as biosensors
Aptamers are short, single strands of artificial nucleic acids which bind to specific targets. This binding is similar to the binding exhibited by antibodies but aptamers offer improved properties and consequently are replacing antibodies in some applications. This first part of this project …
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Selective targeting to glioma with nucleic acid aptamers
… glial cells. Malignant glioma are characterised by a rapid growth rate and high capacity for invasive infiltration to surrounding brain tissue, hence diagnosis and treatment is difficult, and patient survival is poor. Aptamers are small molecular ligands composed of short oligonucleotides that …
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Functional DNA Aptamers as Biotherapeutic Molecules
… DNA or RNA, which can bind to a myriad of targets such as ions, peptides, proteins, drugs, organic and inorganic molecules with high affinity and specificity. Aptamers are derived using combinatorial libraries comprised of a variable region flanked by two primer regions used for a …
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Engineered mRNA regulation using an inducible protein-RNA aptamer interaction
The importance and pervasiveness of naturally occurring regulation of RNA function in biology is increasingly being recognized. A common regulatory mechanism uses inducible protein-RNA interactions to shape diverse aspects of cellular RNA fate. Recapitulating this using a novel set of protein-RNA …
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Dna Aptamers Selected Against Wild-Type Helix 69 Ribosomal Rna And Their Implications In Combating Antibiotic Resistance
<p>Outbreaks of advanced antibiotic-resistant strains of microbes have hastened the need to identify new viable molecular targets for the development of novel anti-infectives. For this purpose, helix 69 (H69, or m3a 19-nucleotide (nt) hairpin motif that is highly conserved throughout …
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Discovery of Peptide and Aptamer Ligands for Targeted Drug Delivery to Hepatic Stellate Cells
The objective of this dissertation is to present two approaches to identify Hepatic Stellate Cells (HSCs) targeting ligands using phage-display library biopanning and systematic evolution of ligands by exponential enrichment (SELEX). These HSC-specific ligands can be used for diagnosis and therapy …
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G-Triple Chemiluminescent DNAZyme Based Biosensor for the Detection of Calcium(II) Ions in Solution and the Biological Functionality of Kinase Enzymes and the Rationale for their Employment as Promising Drug Targets, Substrate Specific Inhibition of P38alpha
… structures, which are formed from planar stacks of G-tetrads and follow Hoogsteen hydrogen bonding, have recently received attention as recognition elements in bioanalytical assays. DNAzymes are functionally active, synthetic single-stranded DNA molecules that are capable of catalyzing biological …
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I. In vitro selection of aptamers for perchlorate and melamine II. Towards crystallization of DNAzymes III. A highly selective lead sensor based on a classic lead DNAzyme
… transfer, as well as regulating various aspects of biology. The discovery of in vitro evolved functional nucleic acids opens a new field for nucleic acid chemistry and applications. The first in vitro isolated functional nucleic acid was discovered in 1990 through a process known as SELEX, …