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Showing 1 to 3 of 3 for “"synthetic probe"”.
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Protein Semi-Synthesis in Vivo
<p>Incorporation of chemical probes into proteins is a powerful way to elucidate biological processes and to engineer novel function. This thesis describes the ligation of synthetic molecules to target proteins in an intracellular environment, and the use of semi-synthetic proteins for whole animal …
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STUDIES ON THE EFFECTS OF SOME FLAVONOIDS ON DNA TRANSCRIPTION AND CLONING OF LIPOAMIDE DEHYDROGENASE GENE
… of the library with a 32-P labelled 27 mer synthetic probe. Enzymological studies indicated the presence of a correctly inserted gene which is expressed following IPTG induction. Double digestion with restriction enzymes KpnI and SstI showed the presence of a 1.5 kb insert. Further …
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Toward the Synthesis of Nuclease Models.
<p>Nucleases are enzymes that can specifically recognize nucleic acids and hydrolyze their phosphodiester bonds effectively. As is the case with many hydrolases, nucleases often carry one or more metal centers. Cooperation between such metal centers and other interactions involving general …