Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 11 of 11 for “"synthetic ligand"”.
-
Natural and Synthetic Ligand-Binding Induce Different Pilin Assemblies in vitro and Control P. aeruginosa Bioactivities in vivo, and Development of Bacterial-Motility Enabled Binding Assays
… of the pili appendage upon making contact with a ligand or surface, as well as caused by chemical signals within the bacterium. Despite all the functions of pili identified, the natural and synthetic ligands specific for pilin proteins remain elusive. In this research, we report natural and …
-
Lipid sensitivity of the GPR132 receptor
… were used to identify a binding site for these ligands and validated using receptor mutagenesis. Interestingly, mutation of a highly conserved residue on transmembrane domain 5 was found to switch full agonist behaviour of a synthetic ligand towards an inverse agonist. Altogether, the …
-
Gene switches and their applications in eukaryotic systems
… genetic circuits, and they have wide utility in synthetic biology, gene therapy, and developmental biology. Small molecule inducible gene switches are especially valuable as they offer an easy way to regulate target genes using an external signal. By combining DNA binding domain, ligand binding …
-
Allergen Sensitization and Immunomodulation By Synthetic Ligands, Pul-042, In An Allergen-Induced Asthma Murine Model
… mortality. CpG oligodeoxynucleotides (ODNs) is a synthetic ligand that targets Toll-like Receptor (TLR) 9. It has been evaluated as a therapeutic agent for the treatment of cancer, infectious diseases, and for treating allergy and asthma. PUL-042 is also a synthetic TLR ligand and is composed of …
-
Cooperation between peroxisome proliferator activated receptor alpha and delta in regulation of body weight and hepatic steatosis in mice
… hPPARd mice fed a diet containing PPARd synthetic ligand. This was not seen in animals conditionally-expressing hPPARd?AF2, neither in PPARa-KO or PPARd-KO animals. Concurrently, activation of PPARd in humanised animals caused significant depletion, as compared with controls, of adipose …
-
Developing 1,2,3,4-tetrahydro-5H-aryl[1,4]diazepin-5-ones and Related Scaffolds as Poly-(ADP-ribosyl) Polymerase (PARP) Inhibitors and Exploring Their Targeted Polypharmacology with Kinases
… and Wee) are being tested. In this context, the synthetic lethality of cyclin-dependent kinase 1 (CDK1) and PARP1 is known. Evaluation of PARP1 and CDK1 pharmacophores led to the development of the tetrahydro-arylazepinone (TAAP) scaffold as a potential dual PARP1/CDK1 inhibitor. We screened a …
-
Engineering Estrogen Receptor-Based Gene Switches and a Superoxide Dismutase for Therapeutic Applications
… alpha (hERalpha) that bind and respond to two synthetic small molecule ligands---4,4'-dihydroxybenzil (DHB) and 2,4-di(4-hydroxyphenyl)-5ethylthiazole (L9)---with high specificity and potency. The resulting highly specific DHB/7-S receptor and L9/L7-E receptor pairs exhibit nanomolar or …
-
Cyclic loading of fibronectin coated beads induces differentiation in mouse embryonic stem cells
… fibronectin (FN) coated magnetic microbeads. The ligand coated magnetic beads were allowed to bind to undifferentiated mES cells plated on 0.6 kPa polyacrylamide gels prior to loading. The cells used were stably transfected with green fluorescent protein (GFP) bound to oct3/4, an important …
-
Cellular reprogramming in eukaryotes
… (DHB), a small molecule, non-steroid synthetic ligand, which acts orthogonally in a mammalian system. After optimization of the gene switch architecture, a VEGF-A induction ratio of ~200× can be achieved in HEK293 cells at micromolar concentrations of DHB. In another example, we report …
-
The functionalisation and application of microporous micro-capillary films for the chromatographic purification of biomolecules
… developing MMCFs functionalised with different ligands and their subsequent testing as chromatography media for bioseparations. MMCFs were functionalised to form weak anion (MMCF-DEAE) and weak cation-exchangers (MMCF-gCM and MMCF-CA). Emphasis was placed on low-cost functionalisation methods …
-
GLYCOMIMETIC LIGANDS TARGETING BACTERIAL LECTINS: RATIONAL DESIGN, SYNTHESIS AND BIOPHYSICAL EVALUATION
… design methods. The synthesized first-generation ligands consisted of a L-fucose core linked to fragments capable of binding to a secondary site on the lectin (referred to as site X), near its natural fucose-binding region. Despite the promising results obtained with these ligands, their affinity …