Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 38 for “"synthetic gene"”.
-
Design of hybrid retroviral/synthetic gene delivery vectors
Gene therapy has the potential to revolutionize healthcare for millions of people. However, it has yet to become a common treatment for the variety of diseases that could benefit from the delivery of therapeutic genes. Limited progress is primarily due to the lack of a safe and efficient means of …
-
Functional Analysis of Synthetic Gene Circuits Controlling A Protein Pump In Yeast
<p>Synthetic biology aims to build biological devices to understand living systems and explore new applications. Synthetic gene circuits such as genetic switches, oscillators and logic gates are at the core of many synthetic biology applications. These gene circuits often include a sensor/regulator …
-
Investigating R gene evolution by meiotic recombination using synthetic gene clusters in Arabidopsis
Plant gene families organized as linked clusters are capable of evolving by a process of unequal crossing-over. This results in the formation of chimeric genes that may impart a novel function. However, the frequency and functional consequences of these unequal cross-over events are poorly …
-
Using Synthetic Gene Clusters to Model Resistance Gene Evolution by Meiotic Recombination in Arabidopsis thaliana
… of constitutively expressed resistance (R) genes. R genes recognize pathogen gene products as signals of invasion. We are interested in how plants evolve R genes to keep pace with rapidly evolving pathogen populations. The mechanisms that drive the evolution of new R genes are poorly …
-
Ligand discrimination and inhibition of heme iron autooxidation by myoglobin: Site-directed mutagenesis of a synthetic gene
Globins have evolved under strong selective pressure to overcome the natural tendency of a free heme prosthetic group to bind carbon monoxide (CO) 1000-fold more tightly than atmospheric dioxygen (O$\sb2$). Without protein-directed discrimination between these ligand molecules, the basal production …
-
Isolation and characterization of a synthetic gene and a genomic clone encoding acyl carrier protein from Escherichia coli
… to E. coli. To investigate this possibility, a synthetic gene encoding ACP was assembled using a novel $lacZ\ \alpha$-complementation test. When provided with the sequences necessary for transcription and translation, the gene was expressed at high levels. Despite the already functional excess …
-
Development of a codon-optimized Latrodectus hesperus MaSp1 synthetic gene for bacterial protein expression using a seamless cloning strategy
… labs across the globe are racing to manufacture synthetic dragline silk fibers. With the production of synthetic dragline silk fibers, there are unlimited commercial applications. In this study, we developed several codon-optimized MaSp1 minifibroin constructs for recombinant protein expression …
-
Minimizing interference due to genetic manipulation
Synthetic gene networks are the functional genetic 'programs' that will form the basis of increasingly sophisticated engineered organisms, cells and tissues. Important factors to consider in biologically engineering circuits are modularity and orthogonality of the components. We explore these …
-
Towards dissecting neural ensembles : development of genetic profiling and targeting approaches
The recent development of genetic neural modulation technologies has brought about a renaissance in systems neurobiology, where manipulation of specific neural ensembles coupled with measurements of the resulting behavioral changes have begun to chart the functional organization of the brain. …
-
Determining The Genotype-Phenotype Connection In Synthetic Inducible Gene Expression Systems
<p><strong>Introduction</strong> Gene expression is an important process whereby the genotype controls an individual cell’s phenotype. However, even genetically identical cells display a variety of phenotypes, which may be attributed to differences in their environment. Yet, even after controlling …
-
Rational Engineering of Expression Level of Multi-Gene Systems encoding Natural Product Biosynthesis in Streptomyces
… genus, harbor unprecedented numbers of biosynthetic gene clusters (BGCs) potentially encoding novel compounds. However, cloning and controlled expression of these large BGCs in a heterologous host require tedious optimization on a case-by-case basis. This dissertation presents a synthetic …
-
A synthetic positive feedback based gene regulatory circuit
… of reengineering and rewiring of pathways and gene regulatory networks for novel uses, and sometimes for mimicking natural systems for various uses in various disciplines gradually acquired the name “synthetic biology”. Synthetic biology, with foundations rooted in multiple disciplines like …
-
Towards extending coarse-grained circuit-host modeling to complex environments
Over the last two decades, synthetic biology has emerged as a critical toolset for genetic engineering. Synthetic gene circuits have been engineered for a variety of uses from functional modules to increasing biosynthetic yield. After decades of showing their potential in labs, synthetic circuits …
-
Entrainment of Bacterial Synthetic Oscillators using Proteolytic Queueing and Aperiodic Signaling
… known as entrainment, is explored using a synthetic biology approach in E.~coli, where I have used rationally designed gene circuits as an experimental model. Two novel modes of entrainment are explored: entrainment by competition between components for degradation, and entrainment by a …
-
Bistability, Synthetic Biology, and Antibiotic Treatment
… focused primarily on cellular mechanisms that generate bistable switches and their impact on cellular dynamics. </p><p>Here, I study emergent bistability due to bacterial interactions with either synthetic gene circuits or antibiotics. A synthetic gene circuit is often engineered by considering …
-
Self-organized Pattern Formation using Engineered Bacteria
… gradients, which are either predefined or generated as part of the patterning processes. However, using E. coli programmed by a simple synthetic gene circuit, I demonstrate here the generation of robust, self-organized ring patterns of gene expression in the absence of an apparent morphogen …
-
Modeling and controlling resource loading in bacterial genetic circuits
Synthetic biology is an emergent interdisciplinary research field whose aim is to engineer biological systems with novel functionalities. Through the addition of synthetic genes, biomolecules are produced that create networks of interactions, referred to as genetic circuits, which endow cells with …
-
Quantitative analysis and characterization of intracellular gene delivery mechanisms
A goal for gene delivery research is to design vectors capable of (a) delivering transgenes to target cells, (b) yielding efficient gene expression, and (c) minimizing any immune, inflammatory, or cytotoxic response. Current research has focused on developing such vehicles using end gene expression …
Page 1 of 2