Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 104 for “"synthases"”.
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Characterization of ceramide synthases (Cers) in mammalian cells
… expression of mRNA for the respective Cer synthases (CerS) and fatty acyl-CoA elongases, as well as changes in the fatty acyl-CoA's of the cells. Therefore, it is clear that Cer subspecies change during embryogenesis, possibly for functionally important reasons. One CerS isoform, CerS2, was …
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Sphingolipid synthases of Toxoplasma gondii and other organisms
… (IPC) synthase to produce IPC. IPC synthases have no mammalian equivalent and have been proposed as targets for anti-fungals and anti-protozoals. In this study sphingolipid scavenging from the host was shown to be non-essential for Toxoplasma proliferation indicating de novo …
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Type III Polyketide Synthases: Discovery, Characterization, and Engineering
The polyketides are a diverse group of natural products with important applications in medicine and industry. Industry, especially the pharmaceutical industry, is under pressure to deliver "greener" chemical syntheses that are less environmentally damaging and incorporate renewable resources. There …
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Design and synthesis of mechanistic probes for polyhydroxybutyrate synthases
… in order to understand the mechanism of PHB synthases (PhaCs). The first type is oxo analogs in which the sulfur in the coenzyme A (CoA) thioester has been replaced with an oxygen atom. A series of 3-R-hydroxybutyryl oxo CoA analogs, (HB)[subscript]nOCoA (n = 1, 2 and 3), were synthesized …
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Head-to-head terpene synthases: Mechanism of action and inhibition
“Head-to-head” terpene synthases catalyze the first committed steps in sterol and carotenoid biosynthesis: the condensation of two isoprenoid diphosphates to form cyclopropylcarbinyl diphosphates, followed by ring opening. In this work, I used x-ray crystallography, and mutagenesis to study the …
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Biochemical Studies of Aromatic Amino Acid Decarboxylases and Acetaldehyde Synthases
… and H2O2 and we named these proteins as DHPA synthases due to the physiological importance of DHPA for cuticle protein crosslinking. Our results provide an efficient way to identify more DHPA synthase enzymes from DDC based on sequence identity and the signature residues we identified (Asn192 …
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Identification of novel components that connect cellulose synthases to the cytoskeleton
… that cortical microtubules aid the cellulose synthases on their way around the cell (Green, 1962; Ledbetter and Porter, 1963). This was proofed in 2006 through life cell imaging (Paredez et al., 2006). However, how this guidance was facilitated, remained unknown. Through a combinatory …
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The System I and System III Holocytochrome c Synthases in Cytochrome c Biogenesis
<p>Cytochromes c are proteins that are involved in important redox reactions in organisms from every kingdom of life. C-type cytochromes, uniquely, possess a covalently bound heme. Since cytochromes c are assembled at their site(s) of function: outside of the cytoplasmic membrane in bacteria, in …
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Biochemical Characterization of Self-Sacrificing P-Aminobenzoate Synthases from Chlamydia Trachomatis and Nitrosomonas Europaea
… the CADD and NE1434 as self-sacrificing pABA synthases that have diverging metal requirements for catalysis.
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Characterization of Cyclopropyl Synthases Involved in the Maturation of Ribosomally Synthesized and Posttranslationally Modified Peptides
… biomarker for the discovery of other cyclopropyl synthases. This novel RiPP modification provided a reaction scope of radical S-adenosylmethionine enzymes.</p>
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PLP-Dependent α-Oxoamine Synthases: Phylogenetic Analysis, Structural Plasticity, and Structure-Function Studies on 5-Aminolevulinate Synthase
… The evolutionary relationships among α-oxoamine synthases representing a broad taxonomic and phylogenetic spectrum have been examined to help identify residues that may regulate substrate specificity. The structural plasticity of ALAS has been documented in studies of functional circularly …
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Cyclization modes in type III polyketide synthases and the synthetic compounds used as probes for mechanistic studies.
Type III polyketide synthases (PKS) catalyze different cyclization reactions of linear polyketide intermediates to produce polyketides with distinct ring structures. The mechanisms of pyrone and resorcinol ring formation in type III PKSs remain unknown, despite many years of research on this family …
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The Origins of Terpene Infochemicals in Insects: Identification and Evolutionary Analysis of Terpene Synthases in Diverse Lineages
Specialized metabolites have important roles as infochemicals in inter- and intraspecific interactions of insects. A particularly abundant class of specialized metabolites are terpenes, which are released by many members of taxonomically diverse insect lineages as pheromone and defense compounds. …
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Mechanistic investigation of polyhydroxybutyrate (PHB) synthases and elucidation of PHB biosynthesis and degradation process in Wautersia eutropha H16
… in the presence of a carbon source. PHA synthases are representative of enzymes involved in polymerizations in which a soluble substrate is transformed into an insoluble inclusion during the polymerization process. The initiation, elongation, and termination phases of this non-template …
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Studies Towards the Discovery of Novel Natural Products Through Functional Analysis of Environmental DNA-Encoded Type II Polyketide Synthases
… products, synthesized by Type II polyketide synthases (PKSs) in soil-borne bacteria. Through the functional characterization of soil eDNA-derived Type II PKS containing clones, herein we attempt to identify novel PKS systems that encode structurally diverse metabolites and explore their …
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Structural insights into microbial one-carbon metabolic enzymes: Ni–Fe–S-dependent carbon monoxide dehydrogenases and acetyl-CoA synthases
Carbon monoxide dehydrogenase (CODH) and acetyl-CoA synthase (ACS) enzymes play crucial rules in the global carbon cycle by catalyzing reversible carbon dioxide reduction and reversible acetyl-CoA synthesis, respectively. In some cases, CODHs are monofunctional, whereas in other cases CODHs form …
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Essential Glycosyltransferases in Schizosaccharomyces Pombe
… human and Caenorhabditis briggsiae Dol-P-Man synthases, all three of which lack a characteristic carboxy-terminal transmembrane region found on previously isolated Dol-P-Man synthases. Known Dol-P-Man synthases can therefore be divided into a 'human' or 'S. cerevisiae' class, although the two …
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Dual-omics approach for 3,4-dihydroisocoumarin characterisation from Hydrangea serrata and isolation of polyketide synthases for heterologous expression in Pseudomonas putida KT2440
… a group of plant specific Type III polyketide synthases. Chalcone synthases and their homologues, the stilbene synthases produce a huge amount of specialised plant metabolites including phenylpropanoids. The dihydroisocoumarin biosynthesis is assumed to be enhanced by elicitors and mediated by …
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