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Showing 1 to 2 of 2 for “"suspension bead array (SBA)"”.
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Identification of Previously Unknown Interactions Between G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins
… a highly multiplexed immunoassay using a suspension bead array (SBA) assay designed to detect RAMP-GPCR complexes. We engineered three epitopetagged RAMPs and 23 epitope-tagged GPCRs, including all members of the secretin-like family of GPCRs, as well as eight other GPCRs. We then used 64 …
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Multiplexed Mapping of the Interactome of G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins
… interactome, we developed a multiplexed suspension bead array (SBA) immunoassay to detect GPCR-RAMP complexes from detergent-solubilized cell lysates. To enable multiplexing, we engineered a library of GPCRs and validated a library of anti- GPCR antibodies (Abs). We first prepared a …