Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"surface marker"”.
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Lymph node and peri-lymph node stroma : phenotype and interaction with T-cells
… The EpCAM+ population has been characterized by surface marker phenotype, anatomic location, and gene expression profile. This population demonstrates the ability to inhibit the activation and proliferation of both CD4 and CD8 T cells. This population may play a role in suppressing overactive …
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The impact of diet-induced obesity and exercise on adipose-resident stem cells
… using the prototypical proteoglycan cell surface marker, neural/glial antigen 2 (NG2), including two pericyte subtypes based on Nestin expression (+/-). Male and female NG2-DsRed x Nestin-GFP reporter mice were placed on control or HFD for 6 wk prior to and following initiation of a 12 wk …
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Understanding the Effect of BVDV on Innate Immune Response of Neutrophils
… infection on viability of neutrophils, their surface marker expression and functional abilities including migration/chemoattraction, phagocytosis, reactive oxygen species production (oxidative burst) and neutrophil extracellular trap (NET) formation. These studies revealed that none of BVDV …
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Effects of Diethylstilbestrol on Murine Early Embryonic Stem Cell Differentiation Using an Embryoid Body Culture System
… development and the expression of cellular surface markers associated with common hemangioblastic and hematopoietic precursors of the endothelial, lymphoid & myeloid lineages were investigated. Hypothesis: Early ES cell expression of CD45 a marker common to lymphoid lineage hematopoietic …
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Erythrocytes derived microvesicles : their characterisation and potential role as delivery vectors
… cell membrane. Immunoblotting and flow cytometry surface marker profiling data shows that eMV are composed of key molecules that may allow their survival in the extracellular environment and communication with neighbouring cells. CD235 surface marker confirms their parent cells and the presence of …
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De novo designed protein switches to enable increased specificity of targeted therapeutics
… rely on the recognition of a single cell-surface marker, and as such, their specificity is limited and prone to incidentally harming healthy cells. This is problematic for developing treatments for solid tumors where ‘tumor-specific’ antigens are also expressed on healthy tissues. Using …
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Identification of a novel metastasis enhancer, CDCP1, and analysis of its functions during melanoma metastasis
… proteins. We found that CDCP1 is not only a surface marker for cells with higher metastatic potential, it is also functionally engaged in enhancing tumor metastasis. When searching for the underlying mechanisms, we found that CDCP1 is important for soft agar colony-forming abilities, …
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Molecular and functional differentiation of murine macrophage subtypes
… wide expression profiling, activation profiling, surface marker profiling, and functional assays testing endocytosis, adhesion, antigen presentation, and cell migration. Surface marker profiling and gene expression analysis revealed similar expression of the macrophage specific markers CD14, …
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Optimization of a pericyte therapy to improve muscle recovery following hindlimb immobilization
… a pericyte therapy is the lack of a unique marker for identification and isolation of pericytes. Investigators have established that pericytes express a wide variety of cell surface markers, such as neural/glial antigen 2 (Cspg4/NG2), melanoma cell adhesion molecule (Mcam/CD146), and …
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Inflammatory astrocyte and T cell interactions are supported by hyaluronan in a murine model of Multiple Sclerosis
… of astrocytes, I identified a novel cell surface marker to identify inflammatory astrocytes. By flow cytometry and confocal microscopy, I determined that the frequency of inflammatory astrocytes is significantly increased in the acute and chronic stages of EAE. I determined that CD4+ T …
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RNA interference targeting Glucose-Regulated-Protein 78 induces HepG2 cell Apoptosis
… therapy has examined a well-characterized cell surface marker that is preferentially expressed on tumor cells. The over-expression of an endoplasmic reticulum chaperone protein, 78-kDa glucose-regulated-protein (GRP78), has been observed on the surface of cancer cells, but not on normal tissues. …
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A novel method of generating Dendritic cells in vitro using the KG-1 cell line and its use as a model for testing effects of lactic acid bacteria
… using both morphological features and cell surface marker expression. Expression of CD83, CD11c, CD123, CD86, HLA-DR and DC-SIGN was assessed by immunofluorescence and flow cytometry. KG-1 cells stimulated with 10 ng/ml PMA and 100 ng/ml Ionomycin were found to be the ideal model for …
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The immunomodulatory properties of helminth-derived products on dendritic cell maturation and function
… alone did not induce cytokine production or cell surface marker expression on DCs, it can maintain the DCs in an immature state, suppressing their function to Toll-Like-Receptor (TLR) stimulation and impairing the subsequent development of adaptive immunity. Furthermore, we selected recombinant …
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A role for polyunsaturated fatty acids in Th1-mediated disease
… by impeding the upregulation of co-stirnulatory markers and MHCII expression. Given the critical role of IL-12 and IL-10 in T helper cell differentiation we looked at the effects of PUFA-modified DC on subsequent T cell development and found them capable of inhibiting IFN-y, IL-17, IL-2, and IL-4 …
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Age-associated alterations in the immune system of normal and autoimmune-susceptible mice
… Ipr mice, age-induced alterations in the cell surface characteristics of the abnormal T cells that accumulate in the lymph nodes were studied. The double-negative T cells from the lymph nodes of old Ipr mice were found to express a cell surface marker, J11d, that is normally present only on …
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Computational Frameworks to Interpret Genetic Abnormalities in Hematologic Malignancies
… detected at the single-cell level and cell surface marker expressions help identify somatic mutations.
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MULTI-OMIC LINEAGE TRACING PREDICTS THE MOLECULAR DRIVERS OF CANCER STEM CELLS IN TRIPLENEGATIVE BREAST CANCER EVOLUTION.
… isolation using TM4SF1 as a TIC-specific surface marker. TM4SF1high cells were confirmed to be enriched for tumor-initiating capacity through in vivo orthotopic transplantation. We further generated high-resolution transcriptional and epigenetic profiles of TM4SF1high cells via bulk …
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Glioblastoma: multifaceted immunosuppression mediated through galectin family members
… B-cells and macrophages. Changes in cell surface marker expression and phagocytosis following exposure to recombinant proteins were assayed using flow cytometry and pHrodo-conjugated Escherichia coli respectively. B-cells and macrophages were predominantly found in tumour bulk (p=0.0053 …
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Cardiomyocyte Cell Cycle, Renewal and Isolation
… a non-genetic FACS based technique utilizing the surface marker VCAM-1, facilitating the enrichment of embryonic cardiomyocytes (paper III). Additional surface markers are assayed for their potential to isolate cardiomyocytes from other time-points in paper IV. This thesis provides new knowledge …
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The mesenchymal regulation of ductal-driven liver regeneration
… and organoid formation in vitro. A cell surface marker screen of the Msc fraction showed labelling of up to 20% of the cells by the stem cell antigen 1 (SCA1). In vivo, SCA1+PDGFRα+ mesenchymal cells localise periportally, closely surrounding biliary duct cells, and co-expanding with them …
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