Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"surface display"”.
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Developing Cell Surface-Display of Enzymes in Thermophilic Geobacillus.
North Carolina State University Theses Microbiology.
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Cell surface display in biomedical applications and biofuels production
Cell surface display allows peptides or proteins to be expressed on the cell exterior as fusions to natural host anchoring motifs. It is a powerful technique with a myriad of applications in protein engineering, environmental bioremediation, biocatalysis, as well as vaccine and therapeutics …
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Development of Bacillus subtilis spores and cells for surface display of proteins
Surface display has attracted the attention of researchers in developing efficient display systems expressing heterologous polypeptides on the surface of bioparticles such as phages, bacterial and eukaryotic cells and bacterial spores. Among these bioparticles, the endospore from B. subtilis has …
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Use of baculovirus surface display for characterization of AHSV4-VP2 antigenic structure
… AHSV proteins. Baculoviruses can also be used to display foreign antigens on the baculovirus envelope surface, which is very beneficial for studying protein-protein interactions. The AHSV major outer capsid protein, VP2, is a good target for subunit vaccine studies as it has been shown to be the …
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Baculovirus surface display of influenza virus haemagglutinin and its potential as a vaccine
… Sub-unit vaccines based on baculovirus surface display technology are an option for the development and generation of safe human vaccines due to the lack of pre-existing immunity, toxicity and ability to replicate in mammalian systems. Furthermore, this technology may provide an …
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DESIGNING A PLATFORM FOR DEVELOPING NOVEL GLYCAN-BINDING PROTEINS USING YEAST SURFACE DISPLAY
… protein scaffold libraries using yeast surface display. A library of the tenth repeat, type III domain of human fibronectin (FN3) was screened against polysialic acid, a glycan found in certain cancers, and enriched using a combination of random mutagenesis, magnetic-activated cell …
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Affinity Maturation of the D1.3 Antibody Using Yeast Surface Display and Flow Cytometry
… affinities attained previously with phage display for anti-protein antibodies, without the necessity for large libraries or site-directed saturation mutagenesis.
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Mechanistic studies of class II lanthipeptide synthetases and yeast surface display of lanthipeptide leader peptides
… of a LanM with the substrate bound to it. Yeast surface display was selected to engineer the LctA leader for better binding affinity to the LctM enzyme. Two rounds of sorting resulted in several mutations in the leader region, including three consistent aspartate mutations. Using fluorescence …
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Engineering of HIV gp120 by yeast surface display for neutralizing antibody characterization and immunogen design
… impossible. In this thesis, we establish a yeast surface display system for the development of gp120-based molecules for antibody characterization and immunogen design. A stripped core gp120 is constructed that retains the correct presentation of the CD4 binding site. Epitopes of several CD4 …
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The development of a flagellin surface display expression system in the gram-positive bacterium, Bacillus halodurans Alk36
… halodurans Alk36, for the over-production and surface display of chimeric gene products. This bacterium harbors the endogenous genetic background to over-produce flagellin protein continuously. In order to harness this ability, key genetic tools, such as gene targeted inactivation, were …
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Increasing Heterologous Protein Secretion From the Yeast S. Cerevisiae Through Manipulation of Cellular Redox Factors
… A system has been set up to utilize the yeast surface display system to screen a cDNA library for factors that increase BPTI surface display, and therefore secretion. The information gathered in this work identifies new directions for increasing protein secretion in yeast.
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A directed evolution approach to engineering recombinant protein production in S. cerevisiae
… in secretory selection. In this thesis, yeast surface display is investigated as a potential proxy for heterologous protein secretion. Although ultimately unsuitable as a screening proxy, the surface display experiments did show a novel method of improving protein secretion by co-expressing a …
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Engineering and Characterization of a Single Chain Antibody Specific for Murine CD28
… of engineered antibody fragments using yeast surface display and flow cytometry was produced. This method allowed for the rapid determination of relative temperature stability without the need to produce large quantities of protein. This method compared favorably to other methods relying on …
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Applications of yeast display in synthetic biology with a focus on biological synthesis and recycling of plastics
Yeast surface display has emerged as a versatile tool in synthetic biology, particularly in protein engineering. This work sought to improve the flexibility and ease-of-use of yeast display for synthetic biologists, as well as applying the yeast display system in several contexts – with a …
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Towards rational design of peptides for selective interaction with inorganic materials
… of the peptide-inorganic interface using Yeast Surface Display. The general approach is to use combinatorial selection to suggest interaction principles followed by rational design to refine understanding. In this pursuit, two material groups-II-VI semiconductors and synthetic sapphire (metal …
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Molecular Engineering of a Single-Chain FV Antibody Fragment to Femtomolar Affinity
… have been isolated from mutagenized libraries displayed on yeast and screened by flow cytometry. Analysis of the nature of mutations conferring binding improvements suggests a potentially general strategy for engineering scFv antibodies for high affinity binding to target ligands. The results …
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Characterizing and engineering antibodies against the epidermal growth factor receptor
… library of random mutants of the EGFR antigen is displayed on the surface of yeast, and the library is combinatorially selected for loss of binding to the antibody being mapped. If a mutant shows loss of binding to an antibody, then that residue is a potential contact residue. In addition, we …
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Programmable Microparticle Scaffolds for Enhanced Diagnostic Devices
… Escherichia coli (E. coli) capable of producing surface display proteins to either anchor the cells, bind to functionalized nanoparticles, or capture small molecules from the environment, all complex actuation features. Additionally, we have created a processing unit that can create signals based …
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Identification and Knockout of Immunodominant Endogenous Retroviral Antigen in Murine Tumor Models
… (env) of eMLV was found to be the dominant cell-surface antigen targeted by iiAbs. Based on this finding, we studied the role of anti-env antibodies in an anti-tumor response. An anti-env antibody termed 1E4 was isolated from a cured mouse by single-cell cloning, and its affinity was improved by …
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The Production of Recombinant Immunoglobulin Superfamily Proteins in the Yeast Saccharomyces Cerevisiae
… using a directed evolution approach and yeast surface display. Evolution resulted in a super-scTCR scaffold with optimized secretion, solubility, and stability properties. This scaffold will, for the first time, enable directed evolution of the binding properties of the scTCR for its …
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