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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"supported lipid bilayers"”.
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Self-assembly of filopodia-like structures on supported lipid bilayers
… (FLSs) by applying frog egg extracts to supported lipid bilayers containing phosphatidylinositol(4,5)bisphosphate, PI(4,5)P 2. The FLSs recapitulate important characteristics of filopodia - they assemble parallel actin bundles from the lipid membrane and they form in the presence of …
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Dynamics and Turnover of Lipid and Protein Domains in Cells and Supported Bilayers
<p>Protein and lipid clustering is an important mechanism for cell processes such as exo- and endo-cytosis and creating functional signaling complexes. It has been seen that both lipids and proteins are involved with the formation of cluster domains on cell membranes, yet little is known about the …
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Influence of protein and lipid domains on the structure, fluidity and phase behavior of lipid bilayer membranes
The lipid bilayer forms the basic structure of the cell membrane, which is a heterogeneous matrix of proteins and lipids that provides a barrier between the interior of a cell and its outside environment. Protein and lipid domains in cell membranes can facilitate receptor localization, stabilize …
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Rate dependent rupture of solid-supported phospholipid bilayers.
An experimental study on solid-supported phospholipid bilayers was performed in order to investigate rate-dependent behavior of force and probability of bilayer rupture. 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) solid-supported lipid bilayers were created on mica using vesicle fusion …
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Bioelectronic and microscopic analysis of bacterial membrane systems with an applied focus on antimicrobial development
… precision. The first is the development of the supported lipid bilayer model that represents bacterial cell membranes in an *in vitro* setting. These models have existed for some years, but recently a breakthrough has been achieved where supported lipid bilayers can now be created which contain …
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Cell-free platform for the functional characterization of intracellular ion transporters
… requires an easily controllable and specific lipid environment that promotes lateral diffusion and protein-lipid interactions. Here, I present a customizable cell-free platform for the functional assembly and integration of membrane proteins in both liposomes and supported lipid bilayers …
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Direct Single Molecule Imaging of Enhanced Diffusion for Enzymes and Enzyme-conjugated Dna origami
… methods (polymer brush coated surface and lipid bilayer coated surface) and different viscosity agents (methylcellulose and glycerol) to slow diffusion and facilitate tracking. We found that high amounts of glycerol inhibited enzyme activity, resulting in the failure to observe the enhanced …
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Domain Redistribution in Lipid Bilayers in the Presence of the Antimicrobial Lipopeptide Fengycin
… microscopy was used to visualize and analyse lipid phase separation within supported lipid bilayers (SLBs) of various compositions. The initial research investigated the influence of protocol parameters on fluorescent probe distribution and lipid domain appearance within SLBs. The influence of …
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Physical-chemical characterization of nanoparticles in relevant biological environments and their interactions with the cell surface
… centrifugation protocols. NP interactions with supported lipid bilayers (SLB) were investigated by QCM-D and neutron reflectometry allowing resolving at the sub-nanometer scale any structural reorganization of the SLB upon NP application. Carboxylated NPs generally caused lipid hydration with …
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Toca-1 driven actin polymerisation at membranes
… formation of filopodia-like structures (FLS) on supported lipid bilayers, Toca-1 is recruited early, suggesting a Toca-1 scaffolding mechanism could precede the recruitment of other actin regulators. One prediction of this model is that Toca-1 would bind proteins previously implicated in …
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Observing, Understanding, and Manipulating Biological Membranes
<p>The phospholipid bilayer is one of the hallmarks of eukaryotic life. This complicated two dimensionally fluid surface is composed of a double layer of lipids which have a region that is hydrophobic and a region that is hydrophilic. The lipid bilayer membranes of a cell act as a barrier that …
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Hybrid organic-inorganic nanomaterials for applications at the biointerfaces
… growth factor), artificial membranes (lipid liposomes and supported lipid bilayers), and cells (neuroblastoma, endothelial cells, retinal pigment epithelial cells). The hybrid nano-bio-interface between the chosen NMs and biomolecules was scrutinized by a multi-technique approach, which …
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Protein segregation and conformation in antigen-receptor triggering: a quantitative fluorescence microscopy study
… lymphocytes are triggered by nickel-chelating lipids in supported lipid bilayers. An effective and easily implemented solution is identified, which can be used in future work with this popular model surface. A short section focusing on the kinase Lck is also included. The feasibility of using …
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The Initial Events of T-Cell Activation in Realistic Model Systems
… the interactions in live T cells. Firstly, supported lipid bilayers (SLBs) as model surfaces for the APC membrane were improved by presenting a glycocalyx barrier and small adhesion protein CD58 alongside pMHC and ICAM-1. It was found that T cells use small membrane protrusions (microvilli) …
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Hydrodynamics and Diffusion of Membrane Proteins to Engineer Chemical Reactions in Synthetic Biomembrane Platforms
Supported lipid bilayers (SLBs) have been used to characterize biological phenomena in membranes and to engineer those phenomena for applications such as sensing and separations. While microfluidic SLB platforms have developed considerably recently, extending the capabilities of such platforms for …
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Identifying the Relevance of C-Reactive Protein Conformers and the Advancement of a Membrane Curvature Binding Assay
<p>Lipid membranes play a vital role in cell signaling processes. Membrane shape and lipid content affect interactions between cellular membranes and proteins. This research focuses on characterizing those interactions and their impact by using various biochemical and biophysical assays. These …
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Neutron scattering studies of water diffusion near the interface of model cell membranes
… depend on the interactions between peptide-lipid domains and the hydrating water. Therefore, the dynamics of the membrane-associated water and its interaction with embedded proteins remain some of the most fundamental issues in biological physics today. Single-supported lipid bilayers …
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USING SINGLE VIRION FUSION ASSAY TO STUDY INFLUENZA VIRUS ENTRY
… (TIRF) microscopy with microfluidics and supported lipid bilayers, making it a very powerful tool for host-pathogen membrane fusion studies. One concern about influenza is that the virus can mutate quickly via three mechanisms (i.e. error-prone RNA replication, influenza reassortment, and …
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Model Membranes Study the Lipid-Reactivity of HIV-1 Antibodies and Vaccine Antigen
… 4E10 are required to first associate with HIV-1 lipids before binding to the target MPER antigen, however, little is known about how lipid membranes contribute to NAb-antigen binding. To this end we have developed model membrane systems to study NAb and antigen lipid interactions. </p><p>We first …
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Memory, metals and neurotrophins
… nanoplatforms for smart drug delivery, supported lipid bilayers formed by neurotrophin peptidomimetics/small unilamellar vescicles adsorption on silica (both planar model and nanoparticles) have been characterized. In conclusion, the interaction of metals and neurotrophins might …
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