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Showing 1 to 20 of 81 for “"sulfhydryl"”.
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The Catalysis of The Reaction Between Iodine and Sodium-Azide by Sulfhydryl Groups
Made available in DSpace on 2014-12-05T23:07:48Z (GMT). No. of bitstreams: 1 0004486.pdf: 4333262 bytes, checksum: 9da5ed32f7cefc31a4e5266d690f0ae4 (MD5) Previous issue date: 1952
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A comparison of sulfhydryl active compounds on activation and germination of Bacillus thuringensis spores
… Bacillus cereus 569 were incubated in various sulfhydryl active compounds in order to compare the effects of these compounds on activation and germination. The sulfhydryl active compounds used were oxidized lipoic acid and cystine (S-S), cysteine HCl and dithiothreitol (S-H). B^. thuringiensis …
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Characterisation of quiescin-sulfhydryl oxidase and nematode astacin mutants using functional studies in caenorhabditis elegans.
… processing of cuticular collagens, the quiescin sulfhydryl oxidases (QSOX), and the nematode astacins (NAS) are of particular interest with regard to cuticle synthesis and proteolytic cleavage of cuticular collagens respectively. QSOX have been shown to be linked directly to the generation of …
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Studies of the coenzyme binding site and essential sulfhydryl group of years 6-phosphogluconate dehydrogenase
… of the role and environment of the essential sulfhydryl group of this enzyme were also performed. N-alkylmaleimides (N-methyl - N-hexyl, inclusive) were shown to inactivate the enzyme, but without a chainlength effect. In the presence of 6-phosphogluconate, the enzyme was protected from …
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A novel diazonium-sulfhydryl reaction in the inactivation of yeast alcohol dehydrogenase by diazotized 3-aminopyridine adenine dinucleotide
… dinucleotide has been found to modify four sulfhydryl groups per molecule of enzyme during the complete inactivation of yeast alcohol dehydrogenase. The reaction of sulfhydryl groups was indicated by titration studies with 5,5'- dithiobis (2-nitrobenzoic acid) as well as isolation and …
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The Role of Sulfhydryl-Containing Low Molecular Weight Ligands for the Environmental Fate of Zinc Sulfide and Metallic Silver Nanoparticles
… silver) that are expected to strongly bind to sulfhydryl-containing ligands (such as cysteine) in the environment. Serine is structurally similar to cysteine, with the only difference of a hydroxyl group in the place of the sulfhydryl group of cysteine. Therefore, serine was used for comparison …
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A characterization of chicken heart mitochondrial malate dehydrogenase
… enzyme at pH 5.0, possibly modifying most of the sulfhydryl groups. N-Ethyl maleimide modification of the enzyme's sulfhydryl groups at pH 4.8 was found to be slightly more specific in its modification than Ellman's reagent. The number of sulfhydryl groups modified by N-ethyl maleimide increased …
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An investigation of the effect of cryoprotective agents on intermolecular disulfide formation
… one possible explanation, on the basis of the sulfhydryl hypothesis. The major question to be answered by this study is: Do cryoprotective compounds which prevent damage in a living system due to freezing also protect proteins against intermolecular disulfide bond formation? The model system …
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Alkylation of rat lens crystallins with iodoacetamide
… with radioactive iodoacetamide in proportion to sulfhydryl content; at a ratio of 1 mg iodoacetamide/mg protein total free-sulfhydryl of the crystallins had reacted after 1 hr at pH 8, 25°C. Alkylated α-, βL-' and y-crystallin fractions demonstrated no altered chromatographic behavior on …
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Chemical and molecular properties of processed soy proteins
… meal which was autoclaved for 20 minutes. Total sulfhydryl content (sulfhydryl plus reduced disulfides) of textured soy equaled that of soy meal, suggesting little destruction or oxidation of sulfur amino acids during hydrolysis. Time after extraction was found to affect the measured sulfhydryl …
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Chemical and physical changes resulting from UHT treatment of milk
… A fluorometric method of analysis for total sulfhydryl content of milk serum was developed that was rapid and sensitive to changes in parts per billion. Quantitative changes in total sulfhydryl at the different ultra-high temperature (UHT) treatments were related to serum protein …
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Intracellular distribution of nitrogen during synchronous growth of Chlorella pyrenoidosa
… was found to be the predominant acid-soluble sulfhydryl-containing compound throughout the cell cycle. Its trend (as % of total cellular-N) was similar to that of TCA-soluble sulfhydryl-containing compounds in synchronized sea urchin eggs. GSH exhibited properties similar to certain compounds …
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The Effects of Selenium on Estrogen-regulated Gene Expression in LNCaP Prostate Cancer Cells
… formation or otherwise complexing with reactive sulfhydryl groups in cellular proteins. The estrogen receptor (ER) contains cysteines in zinc (Zn) fingers that are susceptible to oxidation and internal disulfide formation, which can prevent DNA binding. We examined ER binding to its DNA response …
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Studies of a low molecular weight Zn-containing protein population of lens tissue
Maintenance of reduced protein sulfhydryl groups is an important function of lens metabolism. In an attempt to inter-relate how lens sulfhydryl metabolism, low molecular weight peptides and trace molecular weight Zn-containing protein population was studied. The concentration of rate lens …
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Characterisation of the reaction of 1,4-phenylenebismaleimide with Ca²⁺-ATPase and elucidation of the intramolecular crosslink site
… and myofibrils to allow muscle relaxation. The sulfhydryl crosslinker, 1,4- phenylenebismaleimide, reacts with Ca²⁺-ATPase (110 kD) to form a species with an apparent molecular weight of 125 kD, as well as dimers and high order oligomers, on SDS-P AGE. During the course of this study we have …
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Thioredoxin-mediated reduction of photosynthetic carbon reduction enzymes following chilling in the light
… on the midpoint potential of the regulatory sulfhydryl groups ($-$310 mV), indicating no direct chilling damage to the enzyme. Thiol labeling of FBPase in vivo showed that there was a 60% decrease in sulfhydryl labeling following chilling, indicating that a lower level of reduction of FBPase …
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Covalent Binding and other Mechanisms of Primaquine Toxicity
… metyrapone and piperonyl butoxide), and sulfhydryl-contai ni ng compounds were added to the incubation system. Conversely, the inhibitory effect of inhibitors was not shown on in vivo binding; also induction with phenobarbital decreased the extent of covalent bindi ng in vivo. These …
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Rhenium Carbido Carbonyl Cluster Complexes With Group 12 Metal Centers
… an electron dense, easily detectable label for sulfhydryl sites in proteins.
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