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Showing 1 to 20 of 31 for “"sulfatase"”.
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Structural and Metabolic Studies of Glycosaminoglycans (Sulfatase, Hydrazine, Mucopolysaccharidosis)
A method for chemical cleavage of glycosaminoglycans (GAGs) at N-acetylhexosamine residues was developed. The reaction scheme involved N-deacetylation of the N-acetylhexosamine residues with hydrazine (N(,2)H(,4)) followed by nitrous acid (HONO) cleavage of the polymer at pH 4.0. Initial studies …
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The Development of Sulfatase-Cleavable Linkers for Antibody-Drug Conjugates
… were designed to be cleaved by lysosomal sulfatases, thus revealing a phenolate, primed for 1,6-elimination of a payload within the target cell (Figure 1A). Initially, a panel of model linkers were synthesised using a neopentyl sulfate protecting group. Upon analysis under physiological …
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The relationship of arylsulfatase C and the steroid sulfatases in mammals
Arylsulfatases (arylsulfate sulfohydrolase, EC 3.1.6.1) catalyze the hydrolysis of the O-S bond of sulfate esters. Two types of arylsulfatases have been described based on their subcellular distribution and biochemical properties. Arylsulfatase C, a Type I enzyme, shares many properties with the …
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The Steroid Sulfatase Inhibitor (p-O-Sulfamoyl) - Tetradecanoyl Tyramine (DU-14) Enhances Memory Retention in Passive Avoidance in Rats with Hippocampal Cholinergic Deficit
… via inhibition of the enzyme steroid sulfatase could reverse scopolamine-induced amnesia. p-O-(Sulfamoyl) - tetradecanoyl tyramine (DU-14) is a steroid sulfatase inhibitor (SSI) that prevents the conversion of DHEAS to DHEA thereby increasing the concentration of sulfated neurosteroids …
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Molecular Characterization of pFGE, the Paralog of the C-α-Formylglycine-generating Enzyme
Im aktiven Zentrum von Sulfatasen ist die Seitenkette von Cα-Formylglycin (FGly) die katalytisch aktive Gruppe. In Eukaryonten wird sie durch eine posttranslationale Modifikation der Seitenkette eines in Sulfatasen hoch konservierten Cysteins generiert. Das Enzym, das diese für die Aktivität der …
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In vitro characterization of aromatase, estrone sulfotransferase and estrone sulfatase activities in the porcine placenta and endometrium at 30, 60 and 90 days of gestation
… aromatase, estrone (E1) sulfotransferase and E] sulfatase, in the porcine placenta and endometrium on d 30, 60 and 90 of gestation. These enzymes play key roles in determining in utero concentrations of estrogens. Days 30, 60 and 90 were chosen because previous investigations had determined that …
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Selective Lesion of Cholinergic Neurons of the Septal-Hippocampal Tract: Memory and Learning
… of acquisition of the DMP task.</p><p> Steroid sulfatase inhibitors increase whole brain DHEAS levels, enhance ACh release in the hippocampus, and enhance memory. The present study also investigated the cognitive effects of sulfatase inhibition in SAP lesioned animals. Steroid sulfatase …
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The analysis of a mouse model of Lysosomal Storage Disorder uncovers a role for astrocyte dysfunction in neurodegeneration
… of astrocytes to neurodegeneration in Multiple Sulfatase Deficiency (MS D), a severe Lysosomal Storage Disorder (LSD) caused by mutations in the Sulfatase Modifying Factor 1 (SUMF1) gene. Using Cre/Lox mouse models, I found that astrocyte-specific deletion of Sumf1 in vivo induced severe …
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The Substrate Specificity of Testicular Hyaluronidase and Its Use in Sequencing Chondroitin Sulfates
… Conditions were also designed to eliminate sulfatase activities present in the hyaluronidase preparation. the D-glucuronic acid released also provided a convenient assay of the hyaluronidase activity.
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Metabolism of host-derived carbohydrates by Bifidobacterium breve UCC2003
… Chapter V describes the characterisation of two sulfatase-encoding gene clusters from B. breve UCC2003. The transcriptional regulation of both sulfatase-encoding gene clusters was also investigated. The work presented in this thesis represents new information on the metabolism of host-derived …
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Síndrome de morquio : uma revisão bibliográfica
… of the enzyme N - acetyl galactosamine - 6 - sulfatase causing Morquio syndrome type A, and type B is caused by a deficiency of beta - galactosidase. This syndrome brings as a consequence ske letal disorders, muscle and heart, thus impairing the physical and motor carrier without occurring …
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Post-translational generation of Cá-formylglycine in Prokaryotic Sulfatsases by Radical SAM-Proteins
… ist der katalytische Überrest von sulfatases. FGly wird durch posttranslational Änderung eines Cysteine (Pro- und Eukaryotes) oder des Serins (prokaryotes) gelegen in konserviert erzeugt (C/S)xPxR Motiv. AtsB, ein vorausgesagtes radikales SAM Eisen-Schwefel Protein, ist ein …
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Enzyme Kinetics of Recombinant Human Arylsulfatase B (rhASB)
… enzyme kinetics of recombinant human Arylsulfate sulfatase B (rhASB) by using an in vitro bioassay and direct hydrolysis substrate assay to approach the problem of substrate limitation in establishing a representative kcat for rhASB. The project also explores the makeup of a possible ASB complex …
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Dehydroepiandrosterone and dehydroepiandrosterone sulphotransferase activity and expression in human disease
… catalysed by the microsomal enzyme steroid sulfatase (STS). Conversely, DHEA is converted to inactive DHEAS by the activity of the cytosolic enzyme DHEA sulphotransferase (SULT2A1). However, in addition, evidence is growing that DHEA and DHEAS can have specific, direct effects. In this …
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Identification of up-regulated genes of the hyphomycete, Beauveria bassiana, during infection of Leptinotarsa decemlineata
… genes encode three putative hydrolases (choline sulfatase, serine peptidase, a/b-hydrolase enzyme), three probable transporters (ABC transporter, siderophore transporter, oligopeptide transporter), a presumed metabolic enzyme (pyruvate decarboxylase), a probable V-ATPase, two putative mucin-like …
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Microbial glucosinolate metabolism: Characterization of Enterococcus faecalis XL1 and functional analysis of a GH4 enzyme
… may also involve alternative pathways, including sulfatase-dependent detoxification. Together, these findings expand the understanding of microbial myrosinase-like activity, highlighting E. faecalis XL1 as a novel model for studying redox-driven GSL metabolism and providing new insights into how …
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Glycomics : an emerging paradigm to protein-carbohydrate interactions
… of GAGs using chondroitinase B, HSGAG 2-0 sulfatase and 3-0 sulfotransferase as model enzymes. For each of these enzymes a theoretical model for the enzyme-substrate structural complex is developed and it is coupled with the site directed mutagenesis and biochemical studies to determine its …
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