Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"suicide vector"”.
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Prevalence of the gene lsr2 among the genus Mycobacterium and an investigation into changes in biofilm formation when inactivating lsr2 regulated genes in Mycobacterium smegmatis
… inactivated using homologous recombination via "suicide vector" plasmids lacking a Mycobacterial origin of replication and carrying kanamycin resistance and PCR fragments of the target genes. M. smegmatis Δlsr2 was transformed by electroporation and transformants were plated and screened for …
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The Potential Role for CapB in Pathogenesis of Francisella tularensis
… which was usually transmitted by arthropod vectors or via contact with infected animals. Francisella tularensis subspecies novicida has been used by many researchers in genetic pathogenesis experiments to try to elucidate genes responsible for virulence factors. One of these virulence …
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Characterisation and secretion mechanism of Bordetella pertussis autotransporter proteins
… An allelic exchange procedure with the suicide vector pSS1129 carrying the bap-5 gene disrupted with a kanamycin-resistance cassette was used. PCR and Southern blotting confirmed the replacement of the wild-type bap-5 gene with the mutated version. Moreover, SDS-PAGE and Western blotting …
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Genetic Analysis of Chondroitin Sulfate Utilization in Bacteroides Thetaiotaomicron (Cloning, Mutagenesis, Polysaccharide)
… II gene using a portion of the cloned gene in a suicide vector which I had constructed, pE3-1. The resulting strain was unable to produce chondroitin lyase II. However, it still was able to utilize chondroitin sulfate as its sole source of carbon. To determine if an increase in the copy number of …
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Rhamnose metabolism and metronidazole resistance in B. thetaiotaomicron VPI-5482
… coding sequence of this gene, using the pGERM suicide vector rendered this mutant, B. thetaiotaomicron rhaR", unable to utilize L-rhamnose. Introducing RhaR in this strain, on a plasmid, restored growth in the medium supplemented with L -rhamnose as the sole carbon source, confirming that rhaR …
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The study of cell surface components on Porphyromonas gingivalis
… generated by homologousre combination between a suicide vector carrying a deletion-disrupted allele of pgagl andP. gingivalis chromosomal DNA. The mutant strain (P. gingivalis E51) lacked NAD-dependent glutamate dehydrogenase activity, did not surface-label in an immunogold labelling assay using …
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Site-Directed Mutagenesis in Francisella Tularensis by Allelic
… F. tularensis live vaccine strain. Two suicide vectors for mutagenesis of F. tularensis were constructed based on the commercial PCR cloning vector pSC-A. These vectors were created by inserting into the cloning site a kanamycin resistance gene boarded upstream by 1.3 kb of N-terminal …