Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"subtomogram averaging"”.
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Investigating Microtubules Inside Cells with Cryo-Electron Tomography
… state. I used this method in combination with subtomogram averaging to study intracellular microtubules and attempted to study dynein-driven transport. Starting with tomographic data of mouse axons and *Drosophila melanogaster (Drosophila)* neurites collected by my colleague Helen Foster, I …
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Molecular Architecture of the ER-Mitochondria Encounter Structure
… fluorescence imaging, as well as cryo-CLEM and subtomogram averaging, this work investigates the role of ERMES in mitochondrial fission within yeast and provides a model for the molecular architecture of ERMES from in situ observations. Live-cell imaging experiments reveal that the shape and …
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Activation of lipid kinase VPS34 by Rab GTPases
… on membranes, using electron cryo-tomography and subtomogram averaging. Complex II is dynamic on membranes with only the UVRAG/Beclin 1 “adaptor arm” permanently contacting the outer leaflet of the lipid bilayer. In contrast, the VPS15/VPS34 “catalytic arm” hovers over the membrane and is able to …
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Towards structural characterisation of ciliary rootlets, ciliary caps and intraflagellar transport
… dataset of porcine tracheal cilia combined with subtomogram averaging could further the initial observations of novel membrane-associated densities, IFT-turnaround and the ciliary caps. In interphase cells, centriole-pairs are connected by striated fibrils composed of the coiled-coil protein …
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Structural studies of SARS-CoV-2 spike protein and vesicular coats
… structure of the complex on vesicles by subtomogram averaging, which provides insights into the sites of interaction between GOLPH3 and COPI subunits, as well as their possible cargo interaction.
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Structure & Function of Bacterial Transport Machines in their Cellular Context
… of “ghost” MacAB-TolC containing cells and apply subtomogram averaging. Preliminary results suggest that MacAB-TolC forms an array in cells, and that MacB is structurally flexible, likely in its nucleotide-binding domain. Together, these studies of the MacAB-TolC efflux pump and the T1SS shed …