Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 7 of 7 for “"substrate-trapping"”.
-
The Development of Photo-crosslinkable Trapping Mutants as Tools to Investigate the Interactions of Protein Tyrosine Phosphatases
… to the identity of physiological substrates, and interacting partners in<br/>general, of PRL-3. VHR is a model for a group of atypical dual specificity<br/>protein phosphatases (DUSPs) with a role in cell cycle progression. Only a few<br/>of VHR’s physiological substrates have been …
-
Exploring the Functional Interaction Between CaMK-II and p53
… in the lab using tandem mass spectrometry and “substrate-trapping mutants”, tumor suppressor protein 53 (p53) was identified as a novel CaMK-II binding partner in tissue culture. In this study, I sought to provide characterization of the functional interaction of p53 and CaMK-II. First, a stable …
-
Identification of CaMK-II Protein Targets in Tissue Culture and Zebrafish Embryos using Tandem Mass Spectrometry
… of the tissue-specific binding partners and substrates of CaMK-II which are responsible for specific developmental fates remains a key step in understanding this important protein kinase family. In this thesis research, specific “substrate-trapping” mutants of CaMK-II were designed, …
-
Identification of a Low Molecular Weight Protein Tyrosine Phosphatase and Its Potential Physiological Substrates in Synechocystis sp. PCC 6803
… was examined toward several exogenous protein substrates that had been phosphorylated on either tyrosine residues or serine residues. SynPTP exhibited phosphatase activity toward tyrosine phosphorylated protein substrates (Vmax toward phosphotyrosyl 32P-casein was 1.5 nmol/min/mg). However, no …
-
Non-autonomous regulation of bone mass accrual and the role of T-cell protein tyrosine phosphatase in the bone regulation of insulin sensitivity
… and ability to trap the insulin receptor in a substrate-trapping assay. Here we show that the T-cell protein tyrosine phosphatase (TC-PTP) regulates insulin receptor phosphorylation in the osteoblast, thus compromising bone resorption and bioactivity of osteocalcin. Accordingly, …
-
Dual-specific protein phosphatases in the <i>Archaea</i>
… PTP family in both bacteria and eukaryotes. A substrate trapping protein variant, SsoPTP-C96S/D69A, was constructed to isolate possible SsoPTP substrates present in <i>S. solfataricus</i> cell lysates. Several potential substrates were isolated and identified by mass spectroscopy.
-
Towards understanding substrate recruitment by PP1 holophosphatases
… PP1c, aiding PP1 biogenesis, providing substrate specificity, and localising PP1c. Over 200 non-catalytic subunits for PP1 have been predicted; however the vast majority of these are not characterised. Of the few characterised non-catalytic subunits providing substrate specificity to …