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Showing 1 to 4 of 4 for “"substrate release"”.
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Quantifying the Release of Protein Substrates from AAA+ ATPase ClpX by Single Molecule Total Internal Reflection Fluorescence Microscopy
… by AAA+ proteases typically requires the substrate to be physically unfolded before proteolysis. The efficiency of the unfolding process is hence a critical parameter for determining the turnover rate of the substrate. However, the mechanism by which force is utilized to unfold the protein …
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Structural and Biochmical Studies of Nuclear Import Factor Karyopherin Beta-2
… s have prevented prediction of new Kapbeta substrates. The structure of Kapbeta 2 (also known as Transportin) in complex with its most well studied substrate, the NLS of hnRNP A1 (M9NLS), elucidates the mechanism of substrate release by Ran GTPase. Analyses of the structure in conjunction …
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Characterization of structure and enzymic activity of endo-l, 4-β-D-glucanases purified from Trichoderma viride
… studies. With CM-cellulose as substrate Endoglucanases II, III and IV have specific viscosimetric activities of 1010, 60 and 250 specific fluidity change minute⁻¹ mg protein⁻¹, respectively, and from this substrate release reducing sugars at rates of 29.1, 28.2 and 9.16 µmole …
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Molecular insights into alternating access mechanism of secondary active transporters from molecular dynamics simulations
… gradient to uphill transport of their substrates. To fulfill its function, transporter operates via an alternating-access mechanism, in which it undergoes structural transitions between outward-facing (OF) and inward-facing (IF) states, to translocate substrate from one to the other …