Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"substrate processing"”.
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Dissecting the steps of substrate processing by the energy-dependent protease ClpXP
… for the mechanism of degradation. To degrade a substrate the regulatory component recognizes and binds the substrate, then processes it further for degradation by actively unfolding and translocating it into a sequestered proteolytic compartment. The substrate is degraded into small fragments, …
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Significance of active site residues in the n-domain selectivity of angiotensin-converting enzyme
… domains, each domain displays differences in substrate processing and inhibitor binding abilities. This suggests that active site residues differing between the two domains could provide unique interactions within the N-domain that allow for N-selective binding and processing. Literature …
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On the biosynthesis and discovery of ribosomally synthesized and post-translationally modified peptides
… yet a number of fundamental questions regarding substrate processing remained. Genome mining efforts revealed an uncharacterized TOMM gene cluster in Bacillus sp. Al Hakam (Balh) that contained substrates and enzymes that ultimately overcame previous limitations. Initial reconstitution of the …
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Substrate denaturation and translocation by a proteolytic machine
… protein degradation by binding native protein substrates, denaturing them, and translocating the unfolded molecule into the sequestered proteolytic compartment of its peptidase partner, ClpP. Mechanistic studies of ClpXP degradation provide insight into energy-dependent proteolysis and may help …
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Production of biogas from sugarcane wastes: an assessment of microbial community dynamics for an efficient process
… studies established techniques for the efficient substrate processing and start-up procedure of a biogas process designed for the anaerobic digestion of sugarcane wastes, and by these means provided a highly detailed profile of the microbial community in relation to process parameters.
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Investigation of the Lacticin 481 Synthetase and Its Substrate LctA in the Production of the Antibiotic Lacticin 481
… of several point mutants within its peptide substrate LctA. In an effort to better understand the substrate specificity of LctM such that the enzyme could be utilized to engineer novel therapeutic peptides, further mutational studies were conducted on LctA. Mutation of conserved amino acids …
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Functional and structural studies of AAA+ proteases
… ATPase recognizes, unfolds, and translocates substrates into an associated self-compartmentalized peptidase for degradation. In addition to participating in proteolysis, AAA+ ATPases, by themselves, can unfold and/or remodel macromolecular complexes. However, rigorous characterization of these …
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Design, fabrication, and characterization of a compact deep reactive ion etching system for MEMS processing
… suite of tools that will processes small (~1") substrates and cost less than $1 million. By shrinking the size of the substrate, substantial savings can be realized in material usage, energy consumption, and, most importantly, capital costs. In this thesis, we present the development of the …
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The proteasome and its ancillary proteins
… field mainly focused on the mechanisms by which substrate proteins are specifically tagged for proteasome-mediated degradation and the subsequent mechanisms involved in substrate processing. However, the proteasome is known to interact with a wide range of ancillary proteins, and not all of these …
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Structure and Mechanism of PCAT1, a Polypeptide Processing and Secretion Transporter
… utilize the energy from ATP to transport substrates across biological membranes. Various ABC transporters perform diverse biological functions across all forms of life ranging from importing essential nutrients to exporting toxic drugs (Ford and Beis 2019; ter Beek, Guskov, and Slotboom …