Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"subcellular proteomics"”.
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Dynamic subcellular proteomics identifies novel regulators of adipocyte insulin action
… of the adipocyte insulin-response. First, the subcellular 3T3-L1 adipocyte proteome was mapped under baseline and insulin- stimulated (100 nM, 30 min) conditions using the Localisation of Organelle Proteins by Isotopic Tagging after Differential UltraCentrifugation (LOPIT-DC) method. Over 4000 …
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Using high-content subcellular proteomics to identify x-ray-induced protein trafficking
… and these functions can be dependent on their subcellular localisation. More traditional methods for measuring subcellular localisation of proteins, such as microscopy are lower throughput, are biased/targeted and can be prone to artefacts. In this thesis, a subcellular proteomics technique, …
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… and functional assays but also a shotgun proteomics based analysis of the global proteomes of differentiating mESCs versus EpiSCs. I demonstrate that, although mESCs at the beginning of their differentiation process share common characteristics with both naïve and primed PSCs, they …
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Using high-throughput proteomics to identify novel engineering targets for enhanced rAAV production in HEK293 cells
… flow cytometry. High-throughput expression and subcellular spatial proteomics were then applied to profile the response of HEK293-VP 2.0 cells to rAAV production. The effect of rAAV production on global protein abundance was determined by quantifying HEK293-VP 2.0 cell proteins in mock …
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Safeguarding the mitochondrial genome: identifying components of the mtDNA repair machinery
… MEI-217, MEI-218, and HDM, to investigate their subcellular distribution in Drosophila. Among these proteins, Okra, SPN-B and MEI-217 exhibited potential mitochondrial enrichment. The candidate-focused approach is limited in its ability to screen for large numbers of novel mtDNA repair proteins. …
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CHARACTERIZATION OF PROTEOMIC CHANGES IN CROPS DURING METABOLIC ADAPTATION TO DIFFERENT NITROGEN INPUTS
… to different nitrogen inputs. Among these, proteomics was largely and successfully applied to analyze various aspects, including the role of post-translational modifications and enzyme isoforms. The aim of this PhD project was to obtain new insights about the biochemical events during …