Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 68 for “"subcellular localisation"”.
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Subcellular localisation and functional analysis of UVR8, a UV-B specific signalling component in Arabidopsis
… including: spatial, temporal protein analysis, subcellular localisation studies, structure-function analyses, and the yeast-two-hybrid assay for the identification of UVR8 interacting proteins. To study spatial, temporal and wavelength specific UVR8 protein abundance anti-UVR8 peptide antibodies …
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Cellular Uptake of Cystatin C. Subcellular localisation and intracellular effects of a secreted cysteine protease inhibitor
Cystatin C is a cysteine protease inhibitor, aimed for secretion, as it is produced with a signal peptide. Its target enzymes are thought to be the lysosomal cysteine cathepsins and legumain. Cystatin C has been considered to excert its enzyme inhibiting functions extracellularly, as a defense …
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The characterisation of calpain-like proteins in Trypanosoma brucei
… We also present a comprehensive analysis of the subcellular localisation of selected members of this protein family in trypanosomes. Here, of particular interest is the role of protein acylation for targeting to the flagellum. We show that, although acylation is important for flagellar targeting, …
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Molecular characterisation of the lateral organ boundaries gene (XvLOB) from the resurrection plant Xerophyta viscosa
… the recombinant XvLOB protein expressed, subcellular localisation examined and both the transcript and protein expression levels in response to dehydration was investigated.
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Characterisation and expression of ORF2 in genome segment 10 of African horse sickness virus
… size, nature, expression, function and potential subcellular localisation of the putative protein product AHSV is not known and is investigated in this project. A bioinformatic analysis of all available AHSV Seg-10 sequence data was done. ORF2 was maintained in over 400 AHSV Seg-10 sequences, and …
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The localisation and regulation of phosphatidylinositol-4-phosphate 5-Kinase gamma splice variants and the discovery of a new mammalian splice variant, PIP5KIγ_v6
… is known, for example, the regulation of its subcellular localisation, kinase activity and post-translational modifications. By site-directed mutagenesis and examining more closely several crucial motifs, insight is gained into the putative relationship between the enzyme’s phosphorylation …
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Using high-content subcellular proteomics to identify x-ray-induced protein trafficking
… and these functions can be dependent on their subcellular localisation. More traditional methods for measuring subcellular localisation of proteins, such as microscopy are lower throughput, are biased/targeted and can be prone to artefacts. In this thesis, a subcellular proteomics technique, …
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Functional investigation of PERIOD in the mammalian circadian clock
… that confer 24h organisation to the activity and subcellular localisation of PER-containing complexes. In this study, I aimed to provide a molecular understanding for the rhythmic functions of PER2 in human cells, by first assessing the roles of rhythmic Per transcription and rhythmic PER protein …
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… of a new method for the analysis of protein subcellular localisation, Localisation of Organelle Proteins by Isotope Tagging after Differential ultraCentrifugation (LOPIT-DC). LOPIT-DC can be employed in the future to expand the comparison between distinct states of pluripotency towards an …
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Brain distribution and release of Cholecystokinin octapeptide
… throughout brain, particularly in the cortex. Subcellular fractionation of rat brain was used to study the subcellular localisation of CCK. Tissue was homogenised to shear off nerve terminals (synaptosomes) which were purified and used to study the release of the peptide from hypothalamic and …
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Determining the mechanism of dichloroacetate-regulated processing of the Alzheimer’s disease-related amyloid precursor protein
… investigated the potential effects of DCA on the subcellular localisation of APP and the activity of BACE1. However, none of these potential mechanisms proved to be involved in the DCA mechanism of action. Therefore, in conclusion, the mechanism(s) behind DCA-mediated changes in APP …
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Identification and characterisation of the Spred protein family
… level. Furthermore, we focussed on the cellular localisation and the role of human and murine Spred-2 in the organism. The expression patterns of Spred-1 and Spred-2 differed markedly among various tissues and cell types. In mouse, Spred-1 is abundantly expressed in adult brain, cerebellum, and …
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Localisation of Protein Kinase C in Apoptosis and Neurite Outgrowth
… cells. Induction of apoptosis depends on the localisation of PKCqRD to the Golgi complex, which is mediated by the C1b domain of the protein. Mutation of a single amino acid residue, Met-267 in PKCqC1b, blocks both the Golgi localisation and the apoptotic effect of the PKCqRD. Previous studies …
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Insights into the mechanism of action of quinoline antimalarials against Plasmodium falciparum revealed by novel fluorescent analogues and chemical proteomics
… order to conduct a comprehensive survey of the subcellular localisation of these antimalarials across the entire infected erythrocyte, a suite of novel fluorescent derivatives was designed and synthesised. Key physicochemical properties of these antimalarials were retained in order to preserve …
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Activity-Based Protein Profiling Reveals Changes to the Regulation of Enzymatic Activity by the Hepatitis C Virus
… control of transcription and translation, subcellular localisation, co-factor interactions, or chemical modification to specific amino acids. Activity-based protein profiling allows the potential for activity of target enzymes to be measured, thereby gaining a more accurate representation …
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The streptomyces cytoskeletal protein (Scy) is a key component of the tip organising centre for polarized growth in streptomyces coelicolor
… protein fusions to scy were made and the subcellular localisation of Scy was determined to be strongly at growing hyphal tips. Further clarified here, Scy overexpression can recruit DivIVA protein and the cell wall synthesis machinery to new apical sites. The reciprocal is also shown …
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Investigating tau propagation in situ
… different stages of propagation, including their subcellular localisation and molecular interactions. To achieve this, I employed electron cryo-tomography (cryo-ET), a powerful technique that allows the visualization of biological samples at molecular resolution in near-native conditions. I wanted …
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Developing a novel Drosophila model to study Menin biological function
… syndrome. However, the endogenous expression, subcellular localisation, and protein interactions of Drosophila Menin have not previously been characterised at the protein level. This work describes the generation and validation of a Drosophila line carrying an endogenous 3xHA-tagged allele of …
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Redox Regulation of Type Iα PKA in the Heart
… of substrate sensitises Type I PKA to cAMP. PKA subcellular localisation is determined by regulatory subunits binding A-kinase anchoring proteins (AKAPs), with RIα oxidation enhancing their binding. I assessed whether thioredoxin (Trx) redox recycles disulfide RIα back to its reduced state. In …
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Establishment and regulation of polar growth in Streptomyces
… phosphorylates DivIVA and profoundly alters the subcellular localisation of DivIVA to establish multiple new sites of polar growth. Thereby, AfsK modulates apical growth and lateral branching during normal growth and cell wall stress. I suggest that this is part of a stress response that provides …
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