Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"subcellular compartments"”.
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Spatially-resolved transcriptomic mapping in live cells using peroxidase-mediated proximity biotinylation
… methods for mapping the transcriptomes of subcellular compartments. Here, we developed two methods, termed APEX-RIP and APEX-Seq. APEX-RIP combines peroxidase-catalyzed, spatially restricted in situ protein biotinylation with RNA-protein chemical crosslinking, while APEX-Seq utilizes …
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Characterisation of cyclic adenosine monophosphate/protein kinase A signalling networks in blood platelets
… PKA I and II, were localized in distinct subcellular compartments, with PKA I being identified as a lipid raft protein. Our experimental data suggest that the localization of PKA I to lipid rafts is mediated by interaction with A-kinase anchoring proteins (AKAPs). Additionally, PKA …
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Cellular interactions of the cytolethal distending toxins
… binding, to the trafficking of CDT through the subcellular compartments of the host cell, as a prerequisite for toxin to reach nucleus to exert its cytotoxic effects. My findings have enhanced the understanding of CDT intoxication mechanism and its potential role in the virulence properties of …
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Massively multiplexed imaging probes for comprehensive single-cell analysis
… probes to tag different cell populations, subcellular compartments, specific proteins and nucleotide sequences has enabled examination of cellular phenotypes with increasingly sophisticated detail. Recent efforts to combine physiological imaging and single-cell molecular analysis seek …
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Cellular Trafficking of Single and Multistage Vectors
… is able to dissociate, localizing in different subcellular compartments which can be targeted for exocytosis. In particular the second stage nanoparticles were found to be secreted in microvesicles which can act as mediators of transfer of particles across the endothelium and between different …
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The balance of poly(U) polymerase activity ensures germline identity, survival, and development in Caenorhabditis elegans
… within the germ line and localize to distinct subcellular compartments. In contrast, PUP-3 has a distinct role in germline development. PUP-3 abundance is elevated in the pup-1/-2 double mutant germline, and the loss of PUP-3 activity largely suppresses the pup-1/-2 germline phenotype. We …
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Mitochondrial ATP production and energy-buffering in the human pathogen Trypanosoma brucei
… The TbAK1-3 isoforms are localised in different subcellular compartments, here respectively the flagellum, glycosome and cytosol, and are dependent on the presence of specific organellar targeting signals. Silencing of total TbAK expression in the procyclic form of T. brucei resulted in a …
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Prenylation in cell-intrinsic innate immunity to positive-strand RNA viruses
… response to viruses. Since virtually every subcellular compartment can be utilized by viruses for replication, antiviral factors must also be positioned in these compartments to effectively interact with various viral threats. In this thesis, we explored the impact organelle membrane …
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Preliminary characterization of VmTPT2, a putative monoterpene indole alkaloid (MIA) transporter from Vinca minor L. (Apocynaceae)
… are known to occur in specialized cell types and subcellular compartments. Numerous MIAs display powerful biological activities that have led to their use as pharmaceutical treatments for cancer, hypertension and malaria. Many of these compounds accumulate on the leaf surface of medicinally …
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Spatially precise in situ transcriptomics in intact biological systems
… in their spatial resolution and lack precise subcellular landmarks, limiting the ability to localize transcripts to nanoscale and subcellular compartments. Here, I describe the development of targeted expansion sequencing (targeted ExSeq), a multiplexed RNA imaging method that enables …
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Smart, biocompatible semi-conductor nanocrystal constructs designed for in-vitro imaging applications
… QD-dye FRET sensor is used to track the pH in subcellular compartments along the endocytosis pathway. Lastly, a new microfluidic device is used to deliver QDs into the cell cytosol with high viability and high throughput. QDs delivered this way are shown to be nonaggregated and to interact with …
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The Tansmembrane Associations of HIV-1 Vpu and its Human Targets
… in Vpu-target oligomerization in different subcellular compartments. This work provides new insight into the molecular mechanism of Vpu and furthers our knowledge of membrane protein assembly
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CaV1.3 Ion channel expression and hormone therapy in prostate cancer
… PCa cells but appeared to be localised within subcellular compartments. Treatment of LNCaP cells with AR antagonist, Enzalutamide resulted in the re-localisation of CaV1.3 to the plasma membrane where calcium imaging techniques highlighted the presence of depolarisation-induced calcium …
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IGF2 Promotes Activation of Estrogen Receptors in Basal-like Breast Cancer Cells
… of all BC observed in AA patients. Analyses of subcellular compartments, Western-Blot and siRNA demonstrated that IGF2 activates ER-α and ER-β in ER negative BLBC cells Hs578t and CRL-2335. Our studies show that both IGF-1R and IR crosstalk with ER-α and ER-β promoting BLBC progression. This …
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Effect of rare and common single amino acid substitutions on DISC1 subcellular targeting and functional interaction with ATF4
… is a molecular scaffold residing in multiple subcellular compartments, where it regulates the function of interacting proteins with key roles in neurodevelopment and plasticity. Both common and rare DISC1 missense variants are associated with risk of mental illness and/or brain abnormalities …
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Developing Red Fluorescent Zn2+ Sensors and Characterizing Zn2+ Homeostasis in Mucolipidosis Type IV Disease
… within the same cells or among different subcellular compartments. The excitation wavelength of many RFPs is less phototoxic than that of GFPs which extends imaging time. We demonstrate live cell multi-compartmental imaging of cytosolic and mitochondrial Zn<sup>2+</sup> dynamics using …
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Immunomodulatory effects exerted by Lactobacillus salivarius strains on innate immune cells
… regulating TLR4 signalling originated from subcellular compartments within macrophages. Notably, sustained activation of JAK/STAT cascade and M1-signature genes in TLR2-/- macrophages was ablated by selective TLR4 and JAK inhibitors and by absence of TLR4 in TLR2/4-/- cells. In addition, …
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The cue induced axonal nascent proteome and its translational control mechanisms in neural wiring
… ability to investigate the nascent proteome on subcellular compartments has been hampered by the low sensitivity of existing methodology on quantity-limited samples combined with the difficulty of obtaining sufficient amounts of pure material. By combining pulsed Stable Isotope Labelling by …
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Evaluating convolutional neural networks and transformer architectures for image-based prediction of protein localization in eukaryotic cells
Background: Accurate prediction of protein subcellular localization is critical for understanding protein function and guiding experimental research. Recent advances in deep learning have enabled high-throughput image-based methods to tackle this problem by leveraging large-scale immunofluorescence …
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Dynamic Shuttling of AGO2 to the Nucleus Relieves MicroRNA Repression
… (miRNAs) in the cytoplasm. Localization to subcellular compartments is critical to understanding miRNA action, and our lab and others have shown that miRISC complexes are also present and active in mammalian nuclei. miRISC has several proposed nuclear functions, yet the biological …
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