Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"sub-cellular localization"”.
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GCell A Sub-Cellular Localization Tool
… with a reporting and visualization tool for sub-cellular localization of genes and proteins. Although there is little conservation of the primary structure, the general physiochemical properties are conserved to some extent among proteins that share sub-cellular location. Hence, the function …
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Substrate Interaction and Sub-Cellular Localization in Map Kinase Pathways
Protein kinase cascades control responses to extracellular cues by transmitting signals throughout the cell. Prominent among multifunctional enzymes in kinase cascades are the mitogen-activated protein kinases (MAPKs). Among the various MAPKs identified, the extracellular signal-regulated kinases, …
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Role of Sub-Cellular Localization and Cellular Factors In Regulation of HIV-1 Tat Protein Stability
… for viral replication by modulating a variety of cellular processes. A vast amount of literature is available on the function and effects of Tat, however; intracellular regulation of Tat protein is not precisely understood. In this study, degradation of HIV-1 Tat protein was studied in detail. Tat …
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Purification of SIMPL Antibody and Immunofluorescence of SIMPL Sub-Cellular Localization in Response to TNFα- and IL-1
… of these receptors can result in the nuclear localization of NF-κB where it enables the transcription of several different genes key in the innate immune response. Endogenous co-localization of the SIMPL protein with NF-κB in response to these same cytokine signals has yet to be demonstrated. …
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Characterization of a novel pro-apoptotic role for nuclear associated BCL-2
… glia is to modulate Bcl-2 expression or localization in order to promote an anti-apoptotic function. Likewise, in the case of cancer, 80% of all tumors over-express Bcl-2. While malignant cells utilize mitochondrial localized Bcl-2 to gain protection from apoptosis, possession of Bcl-2 …
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Functional analysis of Smyd1 and Myomesin in sarcomere organization in zebrafish embryos
… is not clear. In this study, we analyzed the sub-cellular localization of Smyd1, the effect of smyd1 knockdown on protein methylation, and the effect of myomesin knockdown on sarcomere organization. We demonstrated that Smyd1b_tv1 is localized to the M-lines of skeletal muscles in zebrafish …
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Evolutionary analysis and functional characterization of the forkhead transcription factor FoxG1
… to non-mammals. Furthermore, changes in the sub-cellular localization of FoxG1 in response to fibroblast growth factor 2 (FGF-2) were investigated in a neural precursor cell line.
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TGF-beta signaling at the cellular junctions
… at the synapses. My results indicate that the cellular junctions define a signaling domain within the plasma membrane, to which TGF-beta signaling machinery is targeted. In the context of epithelial cells, the junctions play a role in TGF-beta signaling regulation through their component …
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THE ROLE OF TIGHT JUNCTION PROTEIN ZO-2 IN THE NUCLEUS AND ITS LINK TO TRANSCRIPTION FACTORS OF THE SLUG/SNAIL FAMILY
… factor was shown to possess functional nuclear localization signals, nuclear export signal and zinc fingers that are important for DNA binding and nuclear retention. Furthermore, ZO-2 may also influence the sub-cellular localization of this transcription factor, for example in wound healing …
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A hair bundle proteomics approach to discovering actin regulatory proteins in inner ear stereocilia
… (MudPIT). The MudPIT analysis yielded a substantial list of proteins. I verified the presence of 21 out of 34 (62%) existing proteins known to be present in stereocilia. This provided strong evidence that my proteomics approach was efficient in identifying hair bundle proteins. Next, I …
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Characterization of a glycerophosphodiester phosphodiesterase in the human malaria parasite Plasmodium falciparum
… To gain insight into the role of PfGDPD, sub-cellular localization was modulated and resulted in a shift in protein distribution, which elicited no growth phenotype. Kinetic analyses suggest PfGDPD activity is Mg₂⁺ dependent and catalytically efficient at the neutral pH environment of the …
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The Role and Regulation of Cyclin G2 in Human Ovarian Cancer Cells
… symptoms, and limited knowledge of the cellular targets that contribute to disease. Cell division is under direct regulation of the cyclin family. Typical cyclins will accumulate periodically to activate cyclin-dependent kinases (CDKs) leading to the unidirectional flow of the cell …
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Characterization of SCF Ubiquitin-Ligase Subunits in Arabidopsis
… encodes the Skp1-like class of polypeptide subunits, where 21 genes have been identified and are known to be expressed in Arabidopsis. The complexity of this family of Arabidopsis Skp1-like--or ASK --genes, together with the close structural similarity among its members, raises the prospect …
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Development of Non-Atp Competitive, Plk1-Selective Inhibitors
… polo box domain (PBD) of PLK1, which modulates sub-cellular localization and mitotic functions of PLK1, we can obtain selective PLK1 inhibition, which can lead to mitotic arrest and inhibit tumor cell proliferation. The structure-guided discovery strategy called REPLACE uses structure activity …
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Inexpensive technologies enabling widespread utilization of image-predicated cell sorting
… phenotypes that can be conveyed through average cellular fluorescence or light scattering levels. However, FACS cannot detect a wide range of phenotypes easily observable through microscopy, such as details of cell morphology, sub-cellular localization, and temporal fluorescence expression with …
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Molecular Mechanisms Underlying Aerobic Respiration and Heme Regulation in Yeast
… of oxygen, as heme synthesis depends on the intracellular levels of oxygen. Yeast and higher eukaryotes have adapted to adverse conditions including low oxygen or hypoxia. To understand the molecular mechanisms underlying hypoxia tolerance on a genome-wide scale, protein localization was studied …
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Regulation of the FGF14:Nav Interactome by a GSK-3 Centered Pathway
… ankyrin-G, β-IV-spectrin, neurofascin, and intracellular fibroblast growth factors (iFGFs, FGF11-14). Dysregulation of the sodium channel complex is associated with a panorama of psychiatric disorders in both animal models as well as human genome-wide association studies. However, the cellular …
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Nature of Large Vesicle Exocytosis in Pancreatic β-cells: Release of ATP and GABA
… The release of peptides and low molecular weight substances was explored by combining the detection of exocytotic events by P2X2 receptor method with imaging of secretory granules. It was found that in rat insulinoma cells these substances were released independently and with different time …
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Effects of BRCA1-Mediated Ubiquitination of HuR on RNA Metabolism in Breast Cancer
… in DNA repair as well as many other important cellular processes. BRCA1, together with BARD1 (BRCA1 Associated RING Domain 1) also functions as an E3 ubiquitin (Ub) ligase. BRCA1 mutation in the RING domain, which is responsible for E3 ligase activity, is associated with increased risk of …
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Molecular characterization of signaling mechanisms in flowering transition in Arabidopsis and identification of novel flowering QTL in soybean
… of TFL1 over-expression. Furthermore, in vivo sub-cellular localization in Nicotiana benthamiana reveals both TFL1 and TIL3/5PTase13 proteins to co-localize in the nucleus and cytoplasm. Finally, Bimolecular Fluorescent Complementation (BiFC) demonstrates in vivo protein-protein interaction …
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