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Showing 1 to 20 of 23 for “"structured illumination microscopy"”.
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Imaging nanoscale pollen morphology with Superresolution Structured Illumination Microscopy
… limit of light are often limited to electron microscopy, which presents significant disadvantages in routine palynological work compared to optical microscopy. Superresolution Structured Illumination Microscopy (SR-SIM) is an emerging method that presents a powerful, non-destructive, and …
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Wide-field structured illumination microscopy for fluorescence and pump-probe imaging
The optical resolution of microscopy is limited by the wave-like characteristic of the light. There are many recent advances in overcoming this diffraction limited resolution, but mostly focused on fluorescent imaging. Furthermore, there are few non-fluorescence wide-field super-resolution …
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Single-Shot Optical Sectioning Using Polarised Illumination Coded Structured Illumination Microscopy (picoSIM)
… wide-eld microscope features a uniform illumination of an extended sample region. A problem arises with this setup since light from outof-focus uorophores is also detected. The result is poor quality in the nal image, as out-of-focus structures appear blurred; furthermore, their emission …
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Theoretical and Experimental Concepts to Increase the Performance of Structured Illumination Microscopy
… implementation, and overall capabilities of structured illumination microscopy (SIM). SIM is a superresolution technique that excels in gentle live-cell volumetric imaging tasks. Many modalities of SIM were developed over the last decade that tailored SIM into the versatile and powerful …
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Deep learning for image processing in optical super-resolution microscopy
Optical microscopy is fundamentally governed by a trade-off between image quality, imaging speed and duration. The quality can be considered a function of the signal-to-noise ratio, contrast and image resolution, which are all limited by the amount of light that can be acquired within a set …
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Methods for optical microscopy beyond conventional limits
Optical microscopy seeks to produce accurate and precise enlarged images of an object. It is highly desirable, particularly in live-cell biological microscopy, that the observation process does not perturb the structure, nor interfere with the dynamics, of the sample. These requirements are at odds …
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High Resolution Phase Imaging using Transport of Intensity Equation
… and tissues. It is complementary to fluorescence microscopy, as such, it can be applied to unlabelled specimens without the need for fluorescent tagging. By quantitatively mapping the phase changes induced in the incident light field by the optical path length delays of the specimen, QPI provides …
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Understanding the Organization, Location, and Interactions of Actin-based Structures During Drosophila Dorsal Closure
… components using total internal reflection structured illumination microscopy (TIRF-SIM). In concert with lattice light-sheet images, I show that when amnioserosa cells are relaxed, actin and myosin form a loose, domed meshwork that protrudes apically from the cellular junctions to which …
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Investigating the aggregation of TDP-43 in models of Amyotrophic Lateral Sclerosis
… pathology in established cell models using microscopy techniques to investigate the relationship of pathological aggregate species and cellular dysfunction. We have characterized stable cell models expressing EGFP-TDP-43 to identify key pathological hallmarks of disease at the molecular …
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Methods for study and manipulation of protein self-assembly
… presents the design and implementation of a structured illumination microscope (SIM) system, which can be used for the study of pathological amyloids and their effects on fast processes in living cells. By utilising a single galvo scan mirror for interferometric pattern formation, as well as …
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Multi-modal imaging of herpesvirus infection
… in detail using fluorescence and electron microscopy, several aspects of the process remain poorly understood. The remodelling of cellular organelles during HSV-1 infection has not been robustly quantitated using three dimensional (3D) volumetric analysis and the involvement of different …
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Re-localization of SNARE proteins in mouse sperm prior to the acrosome reaction
… secondary antibodies. Super resolution Structured Illumination Microscopy (SR-SIM) was used to examine samples collected at 0, 10, 30, 60, and 120 min of capacitation time, to obtain 3D images of SNARE localization. Quantification of the images was completed using western blotting and by …
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Elucidating protein stabilization with fluorescence techniques
… occurs within a few hours (confirmed by structured illumination microscopy (SIM)) and remains stable. Long term stability of the model provides a measurement-supported basis for its use as a reference material in fluorescence studies of protein aggregation.;For free hexameric insulin, …
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Development and application of optical microscopy tools for the study of axon guidance
… In this thesis, state-of-the-art optical microscopy-based tools were developed to improve the efficiency and versatility of commonly used methods for studying axon guidance both *in vitro* and *in vivo*. Furthermore, imaging-based studies were performed on selected guidance cue receptors …
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Surface Functionalisation of Metal-Organic Frameworks for Biomedical Applications
… using powder X-ray diffraction, various microscopy techniques, dynamic light scattering, and zeta potential, among others. These systems were evaluated in PANC-1 pancreatic cancer cells as a proof-of-concept cell line, demonstrating significant biocompatibility and improved cellular …
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Structural Polymorphism of Alpha-Synuclein in Conditions Resembling the Cellular Environment
… were analysed structurally by Atomic Force Microscopy (AFM). The environmental parameters interrogated were high calcium concentrations, (which are particularly relevant in the context of PD pathology), the salts NaCl and KCl (which are the principal ion components of the extracellular and …
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Hardware, software, and applications of super-resolution microscopy
Modern microscopy techniques can image beyond the diffraction limit, in three spatial dimensions, and capture sub-cellular resolution videos, providing new biological insight and assisting in drug development. However, such advanced instruments typically require expert engineers and physicists to …
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Deep Learning For Microscopic Image Restoration
Super-resolution structured illumination microscopy (SR-SIM) provides an up to two-fold enhanced spatial resolution of fluorescently labeled samples compared to conventional widefield microscopy. SR-SIM images are reconstructed by combining several raw SIM images, which were exposed to patterned …
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