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Showing 1 to 20 of 38 for “"structure-function analysis"”.
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Structure-Function Analysis of Insect Olfactory Receptors
… on understanding how the insect olfactory system functions at the periphery, shedding light on the molecular players involved and the interactions between environmental chemicals and OSNs. In Drosophila, most of the ~1,200 OSNs express members of the olfactory receptor (OR) protein family …
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Structure-function analysis of phototropin receptor kinases
… mode of phototropin autophosphorylation and the functionality of a mutated phototropin 1 which demonstrates increased autophosphorylation activity in this system is assessed in planta using transgenic Arabidopsis. It was initially of interest to further evaluate the mode of phototropin …
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Structure-function Analysis of the STRIPAK Complex
… My project focused on the structural and functional characterization of STRIPAK. I mapped direct interactions and interacting regions on these proteins and determined that the PP2A substrate binding subunit (striatin) and CCM3 act as adapter molecules to bridge the kinase and phosphatase …
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Structure-function analysis of 11β-hydroxysteroid dehydrogenase
… key enzyme in the conversion of cortisone to the functional glucocorticoid hormone cortisol. This activation has been implicated in several human disorders, notably the metabolic syndrome where 11β-HSD1 has been identified as a novel target for potential therapeutic drugs. Crystal structures have …
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Structure-Function Analysis of Human Integrator Subunit-4
<p><strong>Structure-function analysis of human Integrator subunit 4</strong></p> <p>Anupama Sataluri</p> <p>Advisor: Eric. J. Wagner, Ph.D.</p> <p>Uridine-rich small nuclear RNAs (U snRNA) are RNA Polymerase-II (RNAPII) transcripts that are ubiquitously expressed and are known to be essential for …
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Structure function analysis of the deubiquitylating enzyme Fam
… (Fam) is a developmentally regulated USP whose function is crucial for mouse pre-implantation development. Fam is expressed in a complex fashion throughout development in a number of diverse tissue types and time points, well beyond its critical role in the early embryo. Fam's orthologue in fly, …
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Structure-function analysis of IntDOT, the CTnDOT integrase
… catalytic arginine, R1. I performed a mutational analysis of IntDOT protein and isolated mutants with substitutions in all three domains of IntDOT proteins, I constructed an E.coli indicator strain that employs the lacI gene as a reporter. The in vivo integration assay using the E.coli indicator …
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Structure-Function Analysis of the EsaR N-terminal Domain
… factors that alter gene expression as a function of ligand detection. This coincides with a high population density and/or a low rate of signal ligand diffusion. The majority of LuxR proteins are activated only in the presence of the signal ligand, an acyl-homoserine lactone (AHL). EsaR, …
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STRUCTURE-FUNCTION ANALYSIS OF THE HUMAN EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1
… to assess their contributions to transporter functionality. Through affinity changes in [ H]NBMPR binding, competitive inhibition profiles with ENT1 inhibitors and [ H]2- chloroadenosine uptake, these residues were found to make minor, but statistically significant, contributions to the …
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Structure-function analysis of the essential islet regulatory factor Nkx2.2
… Defining the factors and their regulatory functions that are required for proper beta cell development will enhance our ability to recapitulate these developmental events in vitro to generate beta cells from alternate cell sources. The homeodomain transcription factor Nkx2.2 is essential …
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Recombinant Hemoglobin Variants: Structure-Function Analysis and Oxygen Therapeutic Design
… polymerized hemoglobin. The clear structural and functional similarity of these variants to native hemoglobin and improved vascular stability suggests that they have potential for use as an oxygen carrying therapeutic.
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Molecular Cloning and Structure-Function Analysis of the Plant Proton-Sucrose Symporter
"To explore other functionally-important domains, the AtSUC1 cDNA was randomly mutagenized and transformed yeast were screened on sucrose- or maltose-limited media for growth. Three point mutations were identified---H65Q, G334S, and L461P. Significantly, they all displayed enhanced sucrose …
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Specificity and structure-function analysis of lupus-derived monoclonal anti-DNA autoantibodies
Specificity and structure-function relationships of the monoclonal anti-DNA autoantibodies, BV 04-01 and BV 17-45, derived from the NZB/NZW F1 lupus-prone murine strain were examined. A single-chain Fv derivative of BV 04-01 was constructed and a series of site-specific mutants were engineered. A …
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Structure Function Analysis of Adenosine Deaminase Acting on tRNA (ADAT2) from Trypanosoma Brucei
… and full-length tRNA. Eukaryotic heterodimers function differently with separate substrate recognition and enzymatic components and are only active on full-length tRNA. Furthermore, in trypanosomatids, the ADAT2/3 complex has been shown to also exhibit C-to-U deamination in vitro, and the ADAT2 …
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Structure-function analysis of HAMLET (human alpha-lactalbumin made lethal to tumor cells)
… suggesting that one protein can have several functions and adjust their structure to meet different structural demands. Changes in tertiary structure have mostly been associated with disease and the most striking example is the prion protein, which changes from a mixed alpha-helical and …
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Structure-function analysis of ZapC, an FtsZ-ring stabilizer, in Escherichia coli cytokinesis
… during early cytokinesis and have overlapping functions in stabilizing FtsZ at midcell, but share no primary sequence identity. The focus of this study is on ZapC, a small cytoplasmic protein that is conserved in the gamma proteobacteria, co-localizes with FtsZ at midcell, and promotes lateral …
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Structure/Function Analysis of the Quorum-sensing Regulator EsaR from the Plant Pathogen Pantoea stewartii
… homologues EsaI and EsaR. Most LuxR homologues function as activators of transcription in the presence of their cognate acylated homoserine lactone signal (AHL). EsaR utilizes an AHL-response opposite of the majority of the LuxR homologues. EsaR represses EPS production at low cell densities. …
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A structure-function analysis of the marine toxin neodysiherbaine and its synthetic analogs on kainate receptors
… excitatory amino acid identified to date. The structure of DH is unique relative to other kainate receptor agonists because it has a glutamate backbone attached to a tetra-substituted hydrofuropyran ring system with two functional groups, at the C8 and C9 positions, that are important …
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Structure-Function Analysis of the Cell Polarity Determinants Bud8p and Bud9p in <i>Saccharomyces cerevisiae</i>
Polarität stellt eine fundamentale zelluläre Eigenschaft von Lebewesen dar und ist eine Grundvoraussetzung für Orientierung und Überleben innerhalb einer mehrdimensionalen Umgebung. In dem eukaryotischen Modellorganismus Saccharomyces cerevisiae, der Bier- bzw. Bäckerhefe, wird Polarität im Rahmen …
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