Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"stromal vascular fraction"”.
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Impact of obesity on stromal vascular fraction in adipose tissue as it relates to ovarian cancer
… abdominal white adipose tissue contains the stromal vascular fraction (SVF) which includes stem and progenitor cell populations, immune cells, and fibroblasts. Since the SVF can also be recruited by the cancer cells, we investigated how obesity affects the survival and metastatic potential of …
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Overcoming wound healing complications following radiotherapy in human breast dermal fibroblasts, through the influence of preadipocytes from the stromal vascular fraction
… TIMP1 and PPAR-γ mRNA (qPCR). Cells from the stromal vascular fraction (SVF) were cultured and characterised by immunocytochemistry and compared to human preadipocytes sourced commercially. The secretion of FGF, adiponectin and VEGF by the preadipocyte and the SVF mesenchymal cells was …
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Characterization of Immune Cell Populations in White Adipose Tissue of Wild Type and Bovine Growth Hormone Transgenic Mice
… in immune cell populations in bGH mice. Stromal vascular fraction (SVF) was collected from three adipose depots. Total SVF cells were significantly increased in all bGH WAT depots as compared to controls. Flow cytometry was used to examine the specific immune cell populations within the …
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Sphingosine-1-Phosphate and Stromal Cells Contribute to an Aggressive Phenotype of Ovarian Cancer
… on ovarian cells and their interactions with the stromal vascular fraction recruited from the adipose tissue in culture conditions that mimic the physiologic environment of the peritoneal cavity. These studies will provide a mechanistic link of obesity, inflammation, and the increased risk of …
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Building an In-vitro Cell Model to Study Cellular Basis of Maturation Timing: A Proof of 3D Cell Culture Methodology in Atlantic Salmon Adipose Tissue
… past smolt stage) was first separated into stromal vascular fraction (SVFs) and mature adipose fraction (MAFs). SVFs contain preadipocytes (precursors of adipocyte cells) in addition to a variety of other cell types. MAFs are mature adipocytes. Both MAFs and SVFs were successfully maintained …
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Adipose-Derived Adult Stem Cells as Trophic Mediators of Tendon Regeneration
The adipose-derived stromal vascular fraction (SVF) is a promising new therapy for equine flexor tendonitis. This heterogeneous population of cells may improve tendon healing via the production of growth and chemotactic factors capable of recruiting endogenous stem cells and increasing …
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Enhancement of Hiv Persistence By Adipose Tissue
… for memory CD4 T cells and viral DNA. Within the stromal-vascular-fraction cellular component of adipose tissues (AT-SVF), activated memory CD4 T cells and provirus were readily detectable in all infected humans (n=5) and rhesus macaques (n=8) so far examined, and at frequencies comparable to …
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Characterization and comparison of cell frequency, growth, and multipotential differentiation of adult mesenchymal stromal cells derived from equine bone marrow and adipose tissue
Equine bone marrow-derived mesenchymal stromal cells (MSCs) and adipose tissue-derived stromal cells (ASCs) were compared for frequency within their respective tissues, cell doubling characteristics and differentiation multipotential in culture based on histochemical staining and compositional …
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Molecular and cellular mechanisms of energy homeostasis in birds
… LWS with more apoptotic cells in the abdominal stromal vascular fraction on day 14 post-hatch, suggesting that apoptosis contributed to lower adipogenic potential and lack of abdominal fat in LWS. Exposure to thermal and nutritional stressors at hatch impaired growth by reducing yolk utilization …
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Metabolic Adaptations of Ovarian Cancer Metastases to Physiological Conditions and Disease Progression
… risk and decrease survival. The recruitment of stromal vascular fraction (SVF) present in adipose tissue represents a growth and proliferation advantage to ovarian tumors, and endogenous sphingolipids like sphingosine-1-phosphate are increased in ovarian cancer patients. These conditions, …
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Mechanisms of growth hormone inhibition of adipose tissue growth
… also by inhibiting adipogenesis. In this study, stromal vascular fraction (SVF) cells were isolated from subcutaneous fat of lit/+ and lit/lit mice and were induced to differentiate into adipocytes in vitro. Oil Red O staining and gene expression analysis revealed that the SVF cells from lit/lit …
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Exploring the metabolic role of GPR30 in mice
… as well as immortalized cell lines and primary stromal cells. We discovered for the first time that GPR30 knockout (GPRKO) female mice were protected from high-fat diet (HFD)-induced obesity, glucose intolerance, and insulin resistance. The decreased body weight gain in GPRKO female mice is due …
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Adipose stromal cells enhance keratinocyte survival and migration in vitro, and graft revascularization in mouse wound healing model
… retention due to infection, hematoma, and low vascularity at the recipient site are other drawbacks associated with the use of skin grafts as a primary treatment for severe burn wounds. As such, research has been done into alternative treatments, which include but are not limited to artificial …
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Anti-Obesity Effects of Adipose-Targeting Resveratrol Nanocarriers
… an anti-obesity potential via inducing adipose stromal stem cells (ASC) differentiation into beige adipocytes in WAT. But its low levels of aqueous solubility, bioavailability, and targeting specificity limit its application in obesity. We have successfully synthesized biocompatible and …
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Identification of ITGBL1: A Novel Regulator of Adipogenesis
… (WAT) leads to insulin resistance and cardiovascular disease. In spite of this clear observation, the mechanisms driving the formation of new adipocytes in obesity remain unclear. The work done up to this point has shown that the presumptive preadipocytes resemble pericytes: mesenchymal cells …
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Characterization and application of adipose derived stem cells following burn injury
… the size of the implanted graft, blood flow and vascular endothelial growth factor (VEGF) (p< 0.05) and Collagen-1 (p<0.01) protein expression in treated animals compared to the controls. Monocyte chemo-attractant protein-1 (MCP-1) mRNA expression was also greater with ASC treatment (p< 0.05). …