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Showing 1 to 20 of 36 for “"stress protein"”.
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Structural studies of the YedU stress protein
The knowledge of stress proteins is important for understanding stress response, pathology of a broad set of diseases, and the development of therapeutics. The Escherichia coli YedU stress protein, also known as Hsp31, is highly induced upon heat shock. To obtain a better understanding for the …
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Structural Determination of the novel Universal Stress Protein G4LZI3 from Shistosoma mansoni using X-ray Crystallography
Structural determination of the novel Universal Stress Protein G4LZI3 from Schistosoma mansoni using X-ray Crystallography Priscilla Masamba PhD (Biochemistry) thesis, Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Zululand Schistosoma mansoni is a …
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Structural Characterization of the interaction between divalent cations and the novel Schistosoma mansoni Universal Stress Protein G4LZI3
… This study took an interest in Universal Stress Proteins and focused on a novel G4LZI3 USP, which has been put forward as a possible candidate vaccine. A sufficient amount (0.518mg/ml) of recombinant G4LZI3 protein was successfully expressed using 0.5mM IPTG and subjected to a Nickel-NTA …
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Water stress protein is secreted, is subject to rapid proteolysis upon rehydration of desiccated cells, and may be glycosylated
A novel and highly-abundant 39-kOa acidic water stress protein, Wsp, has been purified and partially characterized from cells of the desiccationtolerant cyanobacterium <i>Nostoc commune</i> (Scherer, S. and Potts, M. 1989. J. BioI. Chem. 264:12546-12553). In the original study, several …
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Isolation and partial characterization of a water stress protein of the desiccation-tolerant cyanobacterium Nostoc commune UTEX 584 expressed in Escherichia coli
… commune</i> accumulates a novel group of water stress proteins (Wsp) in response to cycles of repeated drying and rehydration. Antibodies, specific for Wsp, were used to screen a lambdafix II library of <i>N. commune</i> UTEX 584 Bam H1 DNA fragments and an 8.5-kb fragment, containing a gene …
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The Universal Stress Proteins of Micrococcus Luteus Are Metabolic Regulators That Confer Resistance to a Diverse Set of Stress Factors
The bacterial stress response systems have developed and evolved over the millennia to deal with the changing environmental conditions experienced by all the bacterial species. A common form of stress mitigation is the development of the dormant phenotype, a process by which bacteria enter a state …
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Regulation of Protein Turnover during Hyper-osmotic Stress in Skeletal Muscle
… study was to examine the effect of hyper-osmotic stress on protein turnover in skeletal muscle tissue using an established in-vitro model. Rat EDL muscles were incubated in either hyper-osmotic (400 ± 10 Osm) or isoosmotic (290 ± 10 Osm) custom-modified media (Gibco). L-[14C]-U-phenylalanine (n=8) …
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Molecular cloning, recombinant protein expression and structural characterization of a universal stress G4LZI3 protein from Schistosoma mansoni
… mansoni has been predicted to encode different stress proteins including the Universal Stress Protein (USP). It has been previously hypothesized that the USP of schistosome parasites have shared protein sequence features which can be useful for inferring their biochemical and environmental …
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Coordination Between Autophagy and the Heat Shock Response: Evidence From Exercise in Animals and Humans
<p>Proteins play a critical role in nearly every biological activity. In consequence, organismal health and homeostasis often hinges on the ability of intracellular regulatory systems to sustain the quality and function of these diverse, structurally complex macromolecules. Correct protein function …
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Iron limitation and the role of Siderophores in marine Synechococcus
… Dome were used to estimate the importance of Fe stress. Genomic and metagenomic measures suggest that iron limitation is, paradoxically, more severe in coastal and upwelling areas than in the open ocean, where iron is less abundant. A serological assay found significant differences in the …
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Redox Sensing By Yeast Hsp70 Facilitates Modulation of Protein Quality Control and The Cytoprotective Response
… of these aggregates is damage sustained to proteins by oxidative stress. Cellular protein homeostasis (proteostasis) relies on the ubiquitous Hsp70 chaperone family. Hsp70 activity has been previously shown to be modulated by modification of two key cysteines in the ATPase domain by …
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Protection of Artemia from vibriosis by heat shock and heat shock proteins
… During my work the application of heat shock proteins (Hsps) as an approach to disease control in aquaculture was explored, with gnotobiotic brine shrimp Artemia larvae as the model organism. The thesis contains several findings, featuring the interesting observation that a non-lethal heat …
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Iron limitation and the role of siderophores in marine Synechococcus
… Dome were used to estimate the importance of Fe stress. Genomic and metagenomic measures suggest that iron limitation is, paradoxically, more severe in coastal and upwelling areas than in the open ocean, where iron is less abundant. A serological assay found significant differences in the …
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Distributions and perturbations of the marine dissolved cobalt cycle in a changing ocean
… were linked to nitrogen, iron and phosphate stress protein biomarkers at the boundaries of oceanographic provinces and upwelling zones, providing insight into the flexibility of cobalt stoichiometry. In both regions, perturbations to the marine cobalt cycle were either predicted or observed; …
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Distributions and perturbations of the marine dissolved cobalt cycle in a changing ocean
… were linked to nitrogen, iron and phosphate stress protein biomarkers at the boundaries of oceanographic provinces and upwelling zones, providing insight into the flexibility of cobalt stoichiometry. In both regions, perturbations to the marine cobalt cycle were either predicted or observed; …
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Novel Insights into the Regulation of Autophagy in Saccharomyces Cerevisiae
… SL-induced autophagy by regulating oxidative stress, protein urmylation and thiolation of cytosolic tRNAs. Future studies will be required to reveal the exact mechanism through which cysteine inhibits SL-induced autophagy. I also discovered that autophagy can be significantly induced upon …
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An in vitro investigation of the molecular effects of aging and exercise signaling within human skeletal muscle cells
… mitochondrial dysfunction and oxidative stress. The purpose of the study was to invesitgate the molecular effects of aging in myoblasts related to cell homeostasis, myogenesis, and mitochondrial biogenesis and whether general exercise signaling via the β-2 adrenergic receptor (β2AR) could …
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Development and application of an antibody-based protein microarray to assess stress in grizzly bears (Ursus arctos)
… The objective of this work was to develop a protein microarray that could detect patterns of physiological stress in a rapid manner with small samples of grizzly bear tissue. Sampling from four regions in the foothills of the Rocky Mountains in Alberta resulted in the capture of 133 bears. …
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Contribution of LEDGF/p75 to Prostate Cancer Chemoresistance
… pathways. We also investigated the role of the stress response protein and transcription co-activator lens epithelium derived growth factor p75 (LEDGF/p75) in HRPC resistance to DTX. Our studies show that LEDGF/p75 overexpression attenuates DTX-induced lysosomal destabilization and cell death in …
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