Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"strand exchange"”.
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Homologous Strand Exchange and DNA Helicase Activities in Plant Mitochondria
… genetic variation in living organisms by exchange and rearrangement of DNA. Most of our knowledge about homologous recombination is limited to processes in bacteria or in eukaryotic nuclei. In E. coli, homologous recombination is dependent on the RecA protein. Higher plant chloroplasts …
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Single molecule fret study on the mechanism of RecA mediated strand exchange
RecA plays a critical role during double strand break repair via homologous recombination. During the strand exchange reaction, RecA forms a helical filament on single stranded (ss) DNA that searches for homology and exchanges complementary base pairs with a homologous double strand (ds) DNA to …
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The molecular bases of tryptophan replacement effects in RecA: A biochemical and biophysical characterization of single- and null- tryptophan mutant Escherichia coli RecA proteins
… coli RecA protein is studied as a prototypic strand exchange protein with structural and biochemical similarity to the archaeal RadA and eukaryotic Rad51 family of recombination proteins. Although the RecA protein has been extensively investigated, structural information characterizing the …
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A genetic analysis of bacteriophage lambda integrase-core site interactions
… The viral protein, Integrase (Int), catalyzes strand exchange between the phage attachment site, attP, and the host target sequence, attB to generate the prophage sites, attL and attR. attP contains the core region, where strand exchange occurs, and the required flanking sequences, the P and …
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A study of DNA-DNA interactions during bacteriophage lambda integrative recombination
… 28 bases long containing either the top strand or the bottom strand sequences of the bacterial attachment site (attB) with or without base substitutions in the core region were chemically synthesized. Annealed oligodeoxynucleotide attB sites containing sequences with perfect base pairs or …
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Characterization of the DMCI and Rad51 homologues and the process of meiosis in trichomonas vaginalis
… is localized to the nucleus. We performed a DNA strand exchange that suggests DMCl may be capable of DNA strand exchange. We also developed a system to determine whether haploid cells of Trichomonas vaginal is are capable of cytoplasmic fusion through the use of fluorescent proteins. …
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Kinetics and regulation of the initial stages of cadherin-mediated adhesion
… held that cadherins mediated binding through exchange of a tryptophan containing strand at the N-terminal domain. Another theory holds that there is an initial equilibrium which then is converted to the strand exchange form. These theories do not fully account for the range of observed …
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A Mutational Analysis of Bacteriophage Lambda Site-Specific Recombination (In Vitro, Dna-Dna Interaction, Protein, Oligonucleotide-Directed)
… occur within the 7-base "overlap" region where strand exchange occurs. In vitro recombination analyses with mutant att sites further demonstrated that the homology dependence involves DNA interactions between one strand in each recombining att site. The in vitro analyses also demonstrated that …
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Characterization of the Integrase of the Bacteroides Mobilizable Transposon NBU1
… -8) and on the bottom (between bases -1 and -2) strands within the 14 bp core region. Previous mutational analysis of the 14 bp showed that certain mismatches within the crossover region of the attN1 site (G(-2)C attN1) or the chromosomal target site (C(-3)G attBT1-1) enhanced the in vivo …
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Single-molecule FRET study on the RecA-mediated DNA repair
… removal. The RecA filament mediates homologous strand exchange resulting in the four-way DNA junction in vivo. We develop single-molecule assays to observe the strand exchange in real time to understand its molecular mechanism. Finally, we explore the conformational repertoire of the four-way …
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Novel Structures of RAD51 Reveal Mechanisms in DNA Damage Repair and Genomic Stability
… stability by promoting the repair of DNA double-strand breaks and the protection of DNA replication forks. RAD51 functions alongside the tumour suppressor protein BRCA2 to catalyse DNA strand-exchange reactions which form an integral part of Homology-Directed Repair. RAD51 has been the subject of …
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The role of mismatch repair and recombination in cellular responses to the DNA damaging anticancer drug Cisplatin
… major cisplatin DNA-adduct, the 1,2-d(GpG) intrastrand crosslink, with equal affinity as a G/T mismatch in the same sequence context. Furthermore, MutS inhibited RecA catalyzed strand exchange reaction at the level of joint molecule formation when the substrate was platinated DNA. In the cell, …
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Single-molecule studies reveal mechanisms of human DNA double-strand break repair
… The most deleterious DNA damages are double-strand breaks (DSBs), and a single unrepaired DSB can lead to cell death. In human cells, there exist two canonical pathways of DSB repair: Non-Homologous End Joining (NHEJ) and Homologous Recombination (HR). Two protein complexes that rapidly bind …
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Functional Analysis of Novel Replication Protein a Homologs in the Euryarchaeota: Implications in the Evolution of Single -Stranded DNA Binding Proteins Across the Three Domains of Life
Single-stranded DNA binding proteins (SSB) play an essential role in all cellular DNA transactions including DNA replication, repair, and recombination. At the replication fork, SSBs serve to stabilize and protect the transient intermediate ssDNA, which is generated by a replicative helicase. …
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Molecular, Biochemical, and Evolutionary Analysis of Single-Stranded Dna-Binding Proteins From the Mesophilic Archaeon Methanosarcina Acetivorans
… across the three domains of life is single-stranded DNA-binding protein. At the replication fork, these proteins bind to stabilize and protect transiently exposed ssDNA from nucleases during DNA transactions. The ssDNA-binding protein is referred to as SSB in bacteria, whereas in eukaryotes …
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Theoretical and computational investigations into structure-function relationships of biomolecular machines at atomic resolution
… and repair, by unwinding nucleic acid strands. Combining bioinformatics approaches and free energy calculations, we characterize how the UvrD helicase changes its conformation at the fork junction to switch its function from unwinding to rezipping DNA. The obtained transition pathway …
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Protein/DNA interactions during site-specific recombination
… sites flanking the region of cleavage and strand exchange on attL and attR as well as six arm-type sites. Two of the arm-type sites are located immediately downstream of the attL core, which is a unique feature of the NBU1 system. In vitro integration assays demonstrated that the DR1a, …