Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"strand displacement"”.
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Towards smart molecular biosensors: Modulation of DNA strand displacement reactions with transcription factors for robust and tunable arsenic quantification
… isothermal DNA amplification, CRISPR-Cas and DNA strand displacement (DSD), have accelerated this transition and the ongoing COVID-19 pandemic has made biological testing ubiquitous. Nucleic acid diagnostics have been at the forefront of this trend, particularly for viral genomes, but increasingly …
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Optimizing the Toehold-Mediated Strand Displacement Reaction on the Nanoparticle Surface by Altering the Surface DNA Density for the Design of a microRNA NanoOptical Sensor
… we examine factors influencing the toehold strand-displacement reaction on nanoparticle surfaces. Gold nanoparticles were functionalized with oligonucleotide sequences with varying surface density by incorporating diluent DNA strands. The hybridization of DNA yields aggregates which then …
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REVERSIBLE QUINONE METHIDE ALKYLATION OF DNA
… preserve QM equivalents by forming labile intrastrand adducts. An oligonucleotide has now been shown to transfer bisQM to its complementary sequences to form interstrand crosslinking. Non-complementary sequences can not be alkylated by bisQM-oligonucleotide adducts. The nucleotide composition of …
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Simulation informed design of stimuli responsive structures and higher-order assemblies in DNA nanotechnology
… emerging from linear amino acid chains. DNA strand displacement reactions allow the design of thermodynamic pathways with controllable kinetics. Meanwhile, the DNA origami technique permits the self-assembly of arbitrary three dimensional structures. Strand displacement pathways have …
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Studies of fluorescent imaging for mRNA detection in living cells
… inducible nitric oxide synthase: iNOS) mRNA by strand-displacement activated Peptide Nucleic Acid: PNA) probes.: 3) Imaging of iNOS mRNA by binary fluorescently labeled PNA probes. The first approach was based on the work of our former lab member Dr. Huafeng Fang, who had constructed a multiple …
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De novo designs of programmable toehold-mediated transcriptional attenuators
… repress gene expression via toehold-mediated strand displacement. My designs ensured the proper formation of the secondary terminator hairpin structure necessary for transcription attenuation and determined a subset of four functional toehold-mediated transcriptional attenuators demonstrating …
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Dynamic DNA Nanotechnology for Probing Single Nucleotide Variants and DNA Modifications
… example, the natural DNA is usually in double-stranded form whereas most hybridization probes aim for single-stranded targets; the concentration of extracted DNA samples is totally unknown thus may lay outside the optimal design of probes/primers. To achieve ultra-high sensitivity and …
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DNA based networks, nanostructures, and meta-stable states
… This mechanism is demonstrated using a "strand displacement strategy" (SDS). Lastly, we employed a novel way of organizing hairpin loop DNA structures based on the orientation of consecutive hairpin loop DNA structures "on top" of one another.
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Scaling up Genetic Circuits in Mammalian Cells: A U1-snRNA-based Platform Enables Mammalian Cells to Compute the Bitwise Inversion of the Square Root of a Number
… nuclear RNAs by the use of the toehold-mediated strand displacement reaction. The transactivated miRNA also does not use any transcriptional regulators or exogenous proteins and, like the miRNA-based NOT gate, has a DNA footprint small enough to fit in a AAV virus.
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Study Of Protein-Rna Interactions Using Fluorescence Resonance Energy Transfer (fret) And Single-Molecule Fret
… the progression of kissing hairpins through strand displacement to an extended duplex formation.</p>
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Programming Molecular Devices using Nucleic Acid Hairpins
… last four decades. They are composed of single strand that is </p><p>hybridized to itself, and the central section forming an unhybridized loop. In nature, they stabilize single stranded RNA, serve as nucleation</p><p>sites for RNA folding, protein recognition signals, mRNA localization and …
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Extracellular Vesicle Capture and microRNA Detection
… enzymatic signal amplification and strand displacement amplification. Then we develop methods for extracellular vesicle lysis and miRNA detection using a one-pot lysis and miRNA capture method. Extracellular vesicles were isolated from matched diseased and normal donor serum. Using …
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EMISSIVE PEPTIDE NUCLEIC ACIDS (PNAS): POTENTIAL TOOLS FOR INNOVATIVE DIAGNOSTICS AND THERAPEUTICS
… able to bind complementary DNA and RNA single strands through Watson-Crick base pairing, with high sequence specificity and affinity2. Thanks to their synthetic nature, PNAs also display high chemical and enzymatic stability3. All these unique features make PNAs excellent candidates for …