Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"stopped-flow kinetics"”.
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Computer interfaced stopped-flow kinetics
Stopped-flow kinetics is a method in which two reactants are forced into a mixing chamber and their flow abruptly stopped. The progress of the reaction is monitored at an observation window, a short distance from the chamber along the flow path. Traditionally, data from the stopped-flow has been …
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Biophysical Analyses of Intermolecular Ion-Pairs in Protein-DNA Complexes
… in DNA target search. Using fluorescence-based stopped flow kinetics experiments, we show an increase in sliding length for the Antp homeodomain in the presence of Ficoll. Furthermore, through NMR spectroscopy-based diffusion experiments, we show that 3-D diffusion is slower for both the Antp …
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Reversible binding of dihydrogen to dinuclear ruthenium complexes containing chelating diphosphines
… by UV-vis and ³¹P{¹H}NMR spectroscopy, and by stopped-flow kinetics; the equilibrium constant and the corresponding thermodynamic parameters, and the forward and reverse rate constants have been determined. The equilibrium is close to being thermally neutral (ΔH° ~ zero), while a ΔS° value of …
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Development and Applications of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry (DESI-MS)
… protein conformation study, fast reaction kinetics study, and the coupling with LC for enhancing protein signal and charge. In liquid sample DESI, direct ionization of continuous-flow liquid samples was achieved without the sample drying. In this way, biological samples can be directly …
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Investigation of the dynamics and conformational changes of the U1A protein
… and specificity, little is known about the kinetics and mechanism of binding. This dissertation aims to help understand the contributions of dynamic processes to the U1A-RNA complex formation. The first chapter introduces the significance of studying RNA-binding proteins, and RNA-Recognition …