Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"stochastic gene expression"”.
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Stochastic gene expression during lineage specification of single T helper lymphocytes
… commitment is regulated. I explored the heterogeneous gene expression during the lineage specification of single T helper cells, by quantitatively measuring mRNA and protein levels. I have discovered a paradigm of cell lineage specification governed by the signaling interplay between …
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Endogenous control of stochastic gene expression in the development of Caenorhabditis elegans
Studies in the past decade have established gene expression as an inherently variable process. Accompanying this exciting finding is a fundamental question: how do physiological events, such as cell fate specification, proceed so robustly in the face of gene expression variability? In this thesis, …
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Stochastic Modeling of Gene Expression and Post-transcriptional Regulation
Stochasticity is a ubiquitous feature of cellular processes such as gene expression that can give rise to phenotypic differences for genetically identical cells. Understanding how the underlying biochemical reactions give rise to variations in mRNA/protein levels is thus of fundamental importance …
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Integrating discrete stochastic models with single-cell and single-molecule experiments
… have noticed that individual cells that are genetically identical behave seemingly randomly in the way they carry out their most basic functions. The field of stochastic single-cell biology has been focused developing mathematical and computational tools to understand how cells try to buffer …
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Modeling heterogeneity in the microbial world
… local microenvironments and in the numbers of gene copies, messenger RNAs, proteins, and other macromolecules that constitute their make up, and importantly, dictate their behavior. In this thesis I will employ the methods of systems biology, stochastic simulation, and mathematical analysis to …
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Phenotypic heterogeneity and evolutionary games in microbial populations
… and architecture of the underlying gene network. This raises the question of what adaptive advantage might be conferred to cells that implement high variation in their decision-making. Currently, the predominant answer in the field is that stochastic gene expression allows cells to …
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Learning from Disorder and Noise in Physical Biology
<p>Stochasticity, disorder, and noise play crucial roles in the functioning of many biological systems over many different length scales. On the molecular scale, most proteins are envisioned as pristinely folded structures, but intrinsically disordered proteins (IDPs) have no such folded state and …
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Nuclear speckle dynamics and function of speckle association in gene expression
… fraction of active chromosome regions and genes. However, their mobility and the regulation mechanism of their number and size are still poorly understood. Importantly, the functional significance of the speckle-gene association also remains unclear. To understand speckle dynamics and …
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Metabolic heterogeneity in microbial populations
… the random nature of the chemistry involved in gene expression. The consequences of this stochastic gene expression are complex and not well understood, especially at the level of large reaction networks like metabolism. Here we investigate how variability in the copy numbers of metabolic …
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Shaping the bacterial transcriptome: Perspectives from kinetic modeling
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01
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Engineering pluripotent stem cell heterogeneity and its effects on differentiation
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-05-01
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Cellular decision making: from phage lambda to stem cells
… and stabilized by the activity of specific genes, but cells can also be induced to switch to alternative states given the appropriate stimulus. Underlying decision making processes that result in different cell states are temporally regulated gene expression cascades. The decision-making …
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Spatiotemporal Characterization of Stochastic Bacterial Growth in Biofilm Environment
… microbiological experiments utilizing genetically engineered Escherichia coli. Before demonstrating the efficacy of these systems, the development of the field of synthetic biology over the past half century is reviewed, focusing on synthetic genetic circuits and their applications …
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"Gene regulation in Escherichia coli beyond the ""rate"" approximation"
… A region of DNA encoding a protein is called a gene. The cell reads the DNA and makes molecular machines made up of proteins to carry out all cellular functions required for survival. All cells live in ever-changing environments, and have different needs at different times. The control of when …
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On the coupling of noisy processes in biology to produce functional phenotypic variability
… Recent studies have demonstrated that both gene expression and physiological processes, such as growth, can be noisy. The cell-to-cell variation resulting from this stochasticity has been implicated in survival strategies for bacterial populations. However, it remains unclear how single …
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Theory of mRNA degradation
… how individual cells achieve a high fidelity in gene expression. Each cell needs to ensure accurate protein levels for its proper functioning and its capability to proliferate. Therefore, complex regulatory mechanisms have evolved in order to render the expression of each gene dependent on the …