Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"stimulated emission depletion"”.
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Adaptive optics stimulated emission depletion microscope for thick sample imaging
… the fluorescent super-resolution techniques, Stimulated Emission Depletion (STED) microscopy has been particularly interesting, as it is a purely optical technique which does not require post image processing. STED microscopy has proven to resolve structures down to the molecular resolution. …
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Stimulated Emission Depletion (STED) Microscopy and Pacific Orange Dye Optimization For H9C2 Cox-1 Imaging Via Indirect Immunocytochemistry
… hindering microbiology and other forms of study. Stimulated Emission Depletion microscopy is one of many new frontiers of microscopy that has recently broken through this diffraction barrier. By utilizing multiple competing sources of light, STED has produced high-resolution, nanoscale images of …
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Nodal photolithography : lithography via far-field optical nodes in the resist
In this thesis, I investigate one approach - stimulated emission depletion - to surmounting the diffraction limitation of optical lithography. This approach uses farfield optical nodes to orchestrate reversible, saturable optical transitions in certain photoresist compounds. After addressing prior …
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Point Spread Function Engineering in Fluorescence Spectroscopy
… verifiziert, dass Entvölkerung durch stimulierte Emission (stimulated emission depletion) dazu verwendet werden kann, zwischenmolekularen Förster-Energie-Transfer in einer Probe zu identifizieren.
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Fluorescence Depletion by Stimulated Emission in Single-Molecule Spectroscopy
This thesis presents the first application of the stimulated emission depletion (STED) technique to the field of single-molecule fluorescence spectroscopy. It is demonstrated that fluorescence quenching induced by STED is reversible and can be repeated a large number of cycles on a single molecule. …
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Dual-Colour STED-microscopy on the Nanoscale
Stimulated emission depletion (STED) microscopy was the first concept for breaking Abbe's diffraction barrier in optical far-field microscopy verified in biological applications. However, the theoretically infinite resolution was limited due to photobleaching of the fluorescent species. In this …
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STED-4Pi Microscopy
Saturated stimulated emission depletion (STED) has been shown to break Abbe's resolution limit in fluorescence microscopy. This technique suppresses fluorescence at the focal rim by use of a depleting laser pulse. In this thesis, theoretical and experimental examinations show that the combination …
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STED microscopy in the visible range
… limited by diffraction. Exploiting a saturated depletion of the molecular excited state by stimulated emission, stimulated emission depletion (STED) breaks the resolution barrier in the important subfield of fluorescence microscopy. To this end, STED microscopy utilizes a doughnut-shaped beam …
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Development and application of correlative STED and AFM to investigate neuronal cells
… combining atomic force microscopy (AFM) with stimulated emission depletion (STED) microscopy was developed. First a custom STED microscope was developed. To facilitate the design of this system the theoretical performance of a range of STED techniques (cw-STED, time-gated STED, pulsed STED and …
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Top-down Cross-Section Controlled III-Nitride Nanowire Lasers
… be potentially used for the atom trapping or the stimulated emission depletion spectroscopy. The polarization-sensitive on-chip optical components also require linear polarization. However, due to the compact size of nanowire lasers and the cross-sectional symmetry of the traditional nanowire …
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Diabetes and the Female Heart
… Finally, I employed super-resolution Stimulated Emission Depletion (STED) microscopy and automated image analysis pipelines to quantify key ECC proteins organisation: transverse tubules (T tubule), ryanodine receptor type 2 (RyR2) clusters, and filamentous actin (F-actin). Results …
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Grenzen und Möglichkeiten des Förster Resonanz-Energietransfer : einzelmolekülfluoreszenzspektroskopische Untersuchung an starren Systemen
… Dazu zählen sowohl die STED-Mikroskopie (engl. stimulated emission depletion) als auch STORM (engl. stochastic optical reconstruction microscopy). Im Gegensatz zu diesen Techniken zählt der Förster Resonanz-Energietransfer (FRET) nicht zu den bildgebenden Verfahren. FRET beruht auf einer …
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Synaptic vesicle recycling <i>in Vivo</i>
… zurück halten. Durch Antikörperfärbung und STED (stimulated emission depletion) Mikroskopie konnte gezeigt werden, dass Vesikelcluster (die hauptsächlich nicht-aktive Vesikel enthalten) die entsprechenden Proteine anreichern, was auch durch Immunoblotting bestätigt werden konnte. In Einklang mit …
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Development of the multi-scale dynamic modeling techniques for multi-modal brain recordings
… our goal. As a super-resolution imaging method, stimulated emission depletion (STED) microscopy has unraveled fine intracellular structures and provided insights into nanoscale organizations in cells. Although image resolution can be further enhanced by continuously increasing the STED-beam …
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Investigating New Guaiazulenes and Diketopyrropyrroles for Photonic Applications
… under acidic conditions, including absorption, emission, quantum yield, and optical band gap patterns. These results were exploited in the design of a photosensitive polymeric system with potential application in the field of three dimensional (3D) optical data storage (ODS). Chapter 2 describes …
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Development of single-cell Raman spectroscopy for noninvasively screening the differentiation of living stem and progenitor cells in biomaterial platforms for 2D and 3D cell culture
… these mechanisms relies on fluorescent markers, stimulated emission depletion imaging, PET scans, and radio labeling. One of the main limitations of these characterization methods is the lack of sustainable detection and fast self-degradation of labeling materials. Motivated by the efforts on …
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Structural characterization of primary cilia using accelerated piezoelectrically driven STED nanoscopy
… a superresolution nanoscopy system using the stimulated emission depletion (STED) technique with novel accelerated piezoelectric control (apSTED). This improved STED system achieved a reduction in photobleaching rates from ~80% to ~10% while maintaining superresolution, ~50 nm at the focal …