Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"sterol transport"”.
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Characterization of ARV1-Mediated Sterol Transport in Yeast and Mammalian Systems
… was initially observed to mediate subcellular sterol distribution, and has since been implicated in the movement of multiple lipid species. In this thesis, we examined the role of ARV1 in S. cerevisiae and mammalian systems by two approaches. In yeast, we used gene deletion to access loss of …
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Purification of Native and Recombinant NPC1
… Type C1 protein (NPC1) is required for the transport of lipoproteinderived cholesterol from lysosomes to endoplasmic reticulum. The 1278-amino acid, polytopic membrane protein has not been purified, and its mechanism of action is unknown. We encountered NPC1 in a search for a membrane …
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Characterizing the Interaction of the ATP Binding Cassette Transporters (G subfamily) with the Intracellular Protein Lipid Environment
Cholesterol is an essential molecule that mediates a myriad of critical cellular processes, such as signal transduction in eukaryotes, membrane fluidity, and steroidogenesis. As such it is not surprising that cholesterol homeostasis is tightly regulated, striking a precise balance between …
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The Role of Mammalian Lipid Transport Protein ORP1 During Coxiella Burnetii Infection
… cytoplasm. While the CCV membrane is rich in sterols, cholesterol accumulation in the CCV is bacteriolytic, suggesting that C. burnetii regulation of lipid transport is critical for infection. The mammalian lipid transport protein ORP1L localizes to the CCV membrane and mediates CCV-ER …
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Characterization of the Functional Role of the Intracellular Cholesterol Transporter StARD4 in Knockout Mice, and Investigation of Epigenetic Modulation of ApoA-I Transcription
<p>Cholesterol is crucial for mammalian survival by playing important roles, such as regulating membrane fluidity and as a precursor for the synthesis of steroid and sex hormones, bile acids, and Vitamin D. In addition, cellular and organismal regulation of cholesterol is important for health. For …
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Characterization of the first steryl ester transporter in biology, Yol075c, and its pleiotropic effects
Sterol transport between the endoplasmic reticulum (ER) and the plasma membrane (PM) in the yeast Saccharomyces cerevisiae occurs through a transport pathway linking the lipid droplet (LD) stored steryl esters and mediated by the ABC transporter Yol075c. Sterols are synthesized in the ER and are …