Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"sterol binding"”.
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Elucidation of the mycosamine dependent sterol binding and antifungal activities of amphotericin B
… that is dependent on the presence of membrane sterols to exert its biological activity. This natural product has been shown to form ion channels in model membrane systems and therefore represents a molecule with the capacity to perform protein-like function, i.e. the formation of a …
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Sterol Binding to Niemann-Pick Type C1 Disease Protein (NPC1) - Implications for its Function in Cholesterol Transport
… many pathways involved in the transport of cholesterol within cells. Despite intense scientific interest, the mechanism by which cholesterol is transported between membrane compartments in animal cells remains obscure. Studies on Niemann-Pick type C disease (NPC) defined the requirement of at …
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Purification of Native and Recombinant NPC1
… for the transport of lipoproteinderived cholesterol from lysosomes to endoplasmic reticulum. The 1278-amino acid, polytopic membrane protein has not been purified, and its mechanism of action is unknown. We encountered NPC1 in a search for a membrane protein that binds 25-hydroxycholesterol …
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Synthesis and function of the conserved motif of mycosamine-containing polyene macrolides
… are thought to be dependent upon the presence of sterols in the cellular membrane, but the precise role of sterols in this mechanism and even whether channel formation and antifungal activity are causatively linked has remained unclear. An advanced understanding of this mechanism thus stands to …
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Electronic tuning of site selectivity and semisynthesis of C2'-deoxyamphotericin B
… channels in phospholipid membranes containing sterol. The resulting membrane permeabilization has long been the leading hypothesis for the primary mechanism of antifungal activity. Three possible interactions involving the carboxylic acid and mycosamine appendages of AmB have been predicted to …
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Biochemical Characterization of Niemann-Pick C: A Disease of Cholesterol Transport
… scientific interest, the mechanism by which cholesterol is transported between membrane compartments in animal cells remains obscure. One transport pathway begins in lysosomes where cholesterol is liberated from plasma lipoproteins that have entered the cell through receptor-mediated endocytosis. …
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A synthesis enabled understanding of Amphotericin B leading to derivatives with improved therapeutic indices
… AmB predominantly exists as an extramembranous sterol sponge that primarily kills yeast and human cells by binding and extracting sterols in a mycosamine dependent fashion. Additionally, the C2’-OH and C3’-NH3+ are critical residues on the mycosamine appendage, which are responsible for …
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Modalities of Cholesterol Binding and Modulation of the NPC Proteins and Scap
… and related plasma lipoproteins deliver cholesterol to cells by receptor-mediated endocytosis. The lipoprotein is degraded in late endosomes and lysosomes, allowing cholesterol to be released. Export of cholesterol from late endosomes and lysosomes (hereafter referred to as lysosomes) requires …