Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"stereocilia"”.
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Understanding Actin Dynamics and Its Regulatory Mechanisms in Developing Stereocilia
Stereocilia, the actin-based protrusions of auditory hair cells, are essential for converting mechanical stimuli into electrical signals. Their formation and maintenance require tightly regulated actin dynamics, including elongation and widening through filamentous actin (F-actin) incorporation. …
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A hair bundle proteomics approach to discovering actin regulatory proteins in inner ear stereocilia
Because there is little knowledge in the areas of stereocilia development, maintenance, and function in the hearing system, I decided to pursue a proteomics-based approach to discover proteins that play a role in stereocilia function. I employed a modified "twist-off" technique to isolate hair …
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Finite-element analysis of inner ear hair bundles: a parameter study of bundle mechanics
… response). Deflection of bundles of hair-like stereocilia extending from these cells has been associated with the transduction process. Stereocilia bundle structure and stiffness controls deflection and thus the fundamental sensitivity of the transduction process. The finite-element method was …
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Modeling human Usher syndrome during Drosophila melanogaster development
… people in the world population. Normally the stereocilia, microvillar protrusions of the apical membrane of inner ear hair cells, are organized into coherent bundles. This precise organization is critical for mechanosensing, i.e. for hearing. Mutation in any of the five known Usher syndrome …
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The Role of Pkd1 in Mouse Inner Ear Hair Cells
… loss and ultra-structural abnormalities of stereocilia that remain properly polarized at adult ages. These findings indicate that PC-1 plays an essential role in stereocilia structure and maintenance, but not directly in MET channel function and planar cell polarity. We also demonstrate that …
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The Role of the Stereociliary Glycocalyx in Hair Bundle Cohesion
… finger-like, membrane-ensheathed projections, stereocilia, emerging from the cell's apical surface. As a structure, the hair bundle is highly conserved, changing little yet performing many functions throughout the vertebrate evolutionary tree. The mechanosensitivity of the hair bundle is …
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Hair Bundle Stiffness in the Turtle Utricle: Structural and Regional Variations
… The stiffest bundles have short kinocilium, tall stereocilia, and ratios of kinocilium to tallest stereocilia height (KS) close to 1. In contrast, the compliant bundles have tall kinocilium, short stereocilia, and KS ratios ranging from 1.6 – 8. The stiffer bundles also tend to have longer array …
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ROLE OF A CONSERVED AMINO ACID MOTIF IN LOCALIZATION OF HUMAN CLIC5 TO MICROVILLI
… The hair bundle is composed of organized stereocilia, which are actin based projections from the apical surface of hair cells in the cochlea. I focus on the role of the protein Clic5, a member of the Clic family, which localizes to the base of stereocilia and is necessary for hearing in …
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Analysis of Vestibular Hair Cell Bundle Mechanics Using Finite Element Modeling
… larger, with more cilia, and also had a higher stereocilia/kinocilium height ratio than the cells in the other group. The stereocilia/kinocilium height ratio was the most important geometric factor in influencing bundle stiffness. Modeling a bundle as just its middle row of stereocilia resulted …
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Low-frequency bias-tone effects on auditory-nerve responses to clicks and tones : investigating multiple outer-hair-cell actions on auditory-nerve firing
… of OHC motility to inner-hair-cell (IHC) stereocilia that is not through the BM. The main thesis objective was to test whether different OHC mechanisms produce AN responses to low-level sounds versus ANIP from mid-to-high-level clicks by comparing the suppressive effects of low-frequency …
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A Computational Study on the Structure, Dynamics and Mechanoelectric Transduction of Vestibular Hair cell
… knowledge of the hair cell structure such as the stereocilia and various extracellular links. In order to observe the dynamic response of hair bundles in the endolymph fluid, I have included fluid drag in the model. Link nonlinearity has been added to reflect recent observations (Tsuprun 2003). …
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Design, Fabrication, and Validation of Membrane-Based Sensors
… cochlea of the ear. Each hair cell contains a stereocilia, which is the primary structure for sound transduction. This study looks to develop and characterize a bilayer lipid membrane (BLM) operated artificial hair cell sensor that resembles the stereocilia of the human ear. To develop this …
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A computational approach for understanding adaptation in vertebrate hair cells
… cell's specialized apical surface called the stereocilia. The current is characterized as having a rapid onset and adapting exponentially with a fast and slow time constant. The fast component is usually attributed to calcium binding directly to the transduction channels to promote channel …
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Understanding The Visual And Auditory Defect In Ush2a Mouse Model
… the retina and in the developing inner ear cell stereocilia. The USH2A gene is very large and attempting gene therapy with conventional viral delivery is not easily attainable due to limited payload capacity of commonly used Adeno-associated viruses (AAV) ( <4.7 kbp.) To understand the mechanism …
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Sound-induced micromechanical motions in an isolated cochlea preparation
… of Corti. In some preparations, individual stereocilia of inner hair cell bundles can be resolved.
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Gentamicin and FM1-43 enter sensory hair cells through mechanosensory transduction channel
… localize the rapid entry to the tips of the stereocilia, and show that perturbations of channel behavior also modulate the dye and drug entry, and that entry requires mechanical gating of the transduction channel. Channel expression in cultured cells also allows dye entry when those channels …
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Tuning of the Active Hair Bundle
… of actin-filled, villous projections (called stereocilia) endowed with assemblies (called adaptation motors) of mechano-sensitive ion channels and myosin molecules that power both spontaneous and evoked movements. Active hair-bundle motility serves two functions: it mechanically amplifies …
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Tension Propagation Along Tip-Link Cadherins: Regulation and Implications for the Auditory System
… mechanosensitive ion channels that sit atop the stereocilia and are gated by tension in tip links. Experimental evidence and theoretical arguments implicate a soft compliant element, with a stiffness of 1-4 mN/m, as necessary for mechanotransduction. Although the identity of this element remains …
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An Open Loop Feed-Forward Control Scheme for Bioinspired Artificial Hair Cell Sensors
… and measured output. In the bilayer with stereocilia test case, RMS power increased with higher excitation levels but the various control laws did not appear to have any discernible impact on output power. In variable frequency testing, sensors were excited from 0-300 Hz to observe the …
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Mutation of SLC7A14 Causes Syndromic Hearing Loss
… mouse cochleae showed degeneration of IHC stereocilia bundles and IHC loss throughout the cochlea. Auditory brainstem response (ABR) thresholds were elevated in Slc7a14-knockout mice, indicative of progressive hearing loss. However, distortion product otoacoustic emission (DPOAE) …
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