Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"stem cell derived cardiomyocytes"”.
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Investigating the effects of enhanced catecholamine stimulation in stem cell-derived cardiomyocytes
… maladaptive remodelling is established. At the cellular level, the remodelling is mediated through the sustained stimulation of β-adrenergic receptors by catecholamines via mechanisms that are not yet clear but proposed to involve altered signalling of molecules such as stress-response growth …
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Development of a bioreactor imaging system for characterizing embryonic stem cell-derived cardiomyocytes
… such as the heart is limited, human embryonic stem cells (hESC) have emerged as a source for potential cardiac therapies. However, despite the use of a variety of biochemical differentiation protocols, current yields of hESC-derived cardiomyocytes (CM) have been low. In the case of hESC-CM, …
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Investigating the role of the cardiac apelin receptor using human embryonic stem cell derived-cardiomyocytes.
… is a lack of a suitable human based in vitro system to investigate the cardiac apelin receptor. Human embryonic stem cells (hESCs) represent a powerful tool for modelling as they are amenable to genetic editing and can be induced to differentiate to any cell type. This study aimed to use …
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Investigating Clozapine-Induced Myocarditis in Stem Cell-Derived Cardiomyocytes from Treatment-Resistant Schizophrenia Patients: A Pharmacoepigenomic Approach
… novel in vitro model, I used induced pluripotent stem cells (iPSCs) derived from patients with treatment-resistant schizophrenia who either experienced clozapine-induced myocarditis (cases) or did not (controls). These iPSCs were differentiated into cardiomyocytes (iPSC-CMs) and exposed to …
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Contractile Properties of Striated Muscle in Development and In Disease
… development and disease at the protein, subcellular, and cellular level. Distal arthrogryposis is a skeletal muscle birth defect that can be caused by a mutation in embryonic myosin; herein, I have found that the continued muscle dysfunction is linked to a delay in relaxation, likely from …
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Modelling Brugada Syndrome using induced pluripotent stem cells
… dermal fibroblasts to induced pluripotent stem (iPS) cells and to differentiate these into cardiomyocytes with the same genetic background provides a novel approach to studying inherited cardiac channelopathies with advantages over existing model systems. Whilst this technique has enormous …
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Delivery of hiPSC Derived Cardiomyocytes via an Injectable Nanofibrous Gelatin Polyurethane Composite Scaffold for Heart Tissue Repair
Cardiac cell therapy, utilizing contractile human pluripotent stem cell-derived cardiomyocytes, including embryonic stem cell-derived (hESC-CM) and induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM), has shown promising results in treating ischemic cardiomyopathy and heart failure. …
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LONG NON-CODING RNA VENTHEART IS NECESSARY FOR VENTRICULAR CARDIOMYOCYTE SPECIFICATION AND FUNCTION
… which influence gene expression and cardiac cell fate. This thesis focuses on verifying and characterizing of one key lncRNA, VENTHEART (VHRT), which was identified as one of the top upregulated markers in cardiomyocyte (CM) development. Utilizing human pluripotent stem cell-derived …
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Effects of the MYH7 R369Q Dilated Cardiomyopathy-causing Mutation on Myosin Crossbridge Kinetics and Cardiomyocyte Contractility
… of this mutation on the contractile system within a cardiomyocyte that results in hypocontractility. To better understand the biochemical and biophysical effects of R369Q mutation, this study utilized CRISPR/Cas9-edited R369Q homozygous human induced pluripotent stem cell-derived …
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Controlling Arrhythmias Using Optogenetic Actuators in Computational Simulations
… (optogenetics) in computational models of heart cells (cardiomyocytes), cardiac tissues, and heart chambers to control arrhythmia development. Chapter 1 presents an overview of cardiac anatomy, relevant physiology, and cardiac electrophysiology computational models. Chapter 2 provides a basic …
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Gatekeeper Connexin43 Phosphorylation Events Regulate Cardiac Gap Junction Coupling During Stress
… reside within the intercalated discs connecting cardiomyocytes, effecting not only direct intercellular electrical coupling, but the localization of other junctional structures and ion channels. Alterations in Cx43 expression occur in essentially all forms of heart disease and is therefore a …
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Cardiac organoid technology and computational processing of cardiac physiology for advanced drug screening applications
<p>Stem cell technology has gained considerable recognition since its inception to advance disease modeling and drug screening. This is especially true for tissues that are difficult to study due to tissue sensitivity and limited regenerative capacity, such as the heart. Previous work in stem …
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Engineered Heart Tissues for Advanced Disease Modeling
Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) are a powerful, in vitro tool for investigating cardiac pathologies. Although hiPSC-CMs have a relatively immature phenotype compared to primary adult cardiomyocytes, their physiological relevance can be improved by …
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Characterization of the cardiogenesis of embryonic stem cells
… as the leading cause of mortality worldwide. Stem cell therapy, aimed to restore contractility and proper vasculature, has gained considerable attention as an attractive therapeutic option. However, proper cell differentiation, survival and integration in an infarcted zone remain elusive. This …
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Microtechnologies for Cardiovascular Tissue Engineering
… of the heart to repair itself, necessitating a cell or organ based therapy to cure the inevitable descent into heart failure. Tissue engineering is uniquely poised to significantly alter this disease burden though the fabrication of cardiac and vascular tissues in vitro. However, the challenges …
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Arrhythmogenic mechanisms of acute cardiac infection
… phase involves viral-mediated remodeling of subcellular structures in the myocardium, which is thought to contribute to arrhythmogenesis. The chronic phase is immune response-mediated, where the host immune system causes damage that induces gross remodeling of the heart, which can result in …
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Biochemical effects of organophosphorous compounds on cultured rat and human cardiomyocyte-like cells
… of organophosphorous compounds (OPs) on cardiomyocytes. The present study aimed to investigate the effects of phenyl saligenin phosphate (PSP), two organophosphorothioate insecticides (diazinon and chlorpyrifos), and their acutely toxic metabolites (diazoxon and chlorpyrifos oxon) on rat …
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Development of cardiac patches using medium chain length polyhydroxyalkanoates for cardiac tissue engineering
… fibres) by growing human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CM) on them. The cells were found to be viable and healthy with comparable beating rates and calcium transients to that measured on gelatin which was used as the positive control. The cell alignment …
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Studies on the role of GPR55 in cardiovascular physiology and pathophysiology.
… (LPI) in cultured rat neonatal ventricular cardiomyocytes provokes distinct cellular functions that are dependent on the location of GPR55, leading to suggestions that GPR55 may regulate cardiomyocyte function at two cellular sites and be a potential therapeutic target for cardiac disorders. …
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Investigating viral subversion of intercellular communication
… directly couple the cytoplasms of apposed cells enabling immunological, metabolic, and electrical intercellular communication. The gap junction protein connexin43 (Cx43; gene name – GJA1) is the most widely expressed human connexin protein and is the predominant connexin in the working …
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