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Showing 1 to 20 of 30 for “"steady-state kinetics"”.

  1. Part I: Investigation of mechanism(s) of Cu(II) and Zn(II) neurotoxicity Part II: Pre-steady state kinetics of the reduction of cytochrome b561 with ascorbate

    Kollalpitiya, Konara Yamuna2011

    wichita-thes

  2. FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE

    Suryadi, JimmyWake Forest University2011

    … we utilize fluorescence spectroscopy and pre-steady state kinetics to unveil some features of MetRS catalysis that were previously unclear.

    wfu Repository record

  3. Mechanistic studies of the class I ribonucleotide reductases from S. cerevisiae and E. coli.

    Ge, Jie, 1976-Massachusetts Institute of Technology2003

    … In the second part of this thesis, pre-steady state kinetics of the class I E. coli RNR is investigated using stopped-flow UV-visible spectroscopy and rapid chemical quench methods. These studies have led to a model in which the rate determining step in catalysis is a physical step prior …

    mit Repository record

  4. Campylobacter jejuni Periplasmic Nitrate Reductase NapA as a Medium for Elucidating Molybdenum Cofactor Reactivity

    Metzger, Mikayla2025

    … this research were site-directed mutagenesis and steady-state kinetics.

    iupui Repository record

  5. Characterization of the meta-cleavage pathway enzymes, 4-Hydroxy-2-ketovalerate aldolase and aldehyde dehydrogenase (acylating), from Pseudomonas sp. strain CF600

    Monastiriakos, Stavroula1998

    … been detected in preparations of DmpG. Second, steady-state kinetics studies of the individual and coupled reactions were performed in order to address the question of coupling. The data obtained are consistent with channeling of the aldolase product, acetaldehyde, to the dehydrogenase active …

    concordia Repository record

  6. Spectroscopic and Kinetic Studies of Hydrodenitrogenation and Hydrodesulfurization over Supported Nickel Phosphide (Ni₂P)

    Gott, Travis MatthewVirginia Tech2008

    … involved in the HDN of pyridine. Transient and steady-state kinetics of a surface intermediate that is formed upon pyridine adsorption and reaction was studied to elucidate the mechanism of HDN over Ni₂P/SiO₂. Additionally, in situ FTIR and X-ray absorption near edge structure (XANES) …

    vt Repository record

  7. Kinetic and mutational studies of two RNA-interacting enzymes

    Casina, Veronica ChristineWake Forest University2011

    Cellular enzymes interact with RNA substrates at several key stages in an organism's life cycle, particularly during the processes of transcription and translation. A more detailed description of enzyme mechanistic features and kinetic parameters is important both for understanding molecular …

    wfu Repository record

  8. Mechanistic Studies and Inhibition of N-hydroxylating Monooxygenases

    Bufkin, Kendra BerniceVirginia Tech2017

    … holo (FAD-bound) and apo (unbound) forms. Pre-steady and steady state kinetics shows that the two forms have different coenzyme preference; apo-AMO prefers NADH while holo-AMO has a higher affinity to NADPH.

    vt Repository record

  9. Experimental and Computational Dynamics of an Aminoacyl-tRNA Synthetase System

    Macnamara, LindsayWake Forest University2017

    … Circular dichroism, binding affinity, and steady-state kinetics were evaluated to determine how the CP domain variants affect the structure and function of the zinc binding knuckle. Molecular dynamics simulations of the tRNA core variants and proline variants were used to investigate …

    wfu Repository record

  10. The glutathione S-transferases : kinetics, binding and inhibition

    Goold, Richard DavidDivision of Medical Biochemistry and Structural Biology1989

    … curves. The most detailed investigations of the steady-state kinetics of glutathione S-transferase have been performed with isoenzyme 3-3 (class Mu) and the substrate 1,2-dichloro-4-nitrobenzene (DCNB). Explanations for the apparently anomalous non-hyperbolic kinetics have included subunit …

    cape-town Repository record

  11. Structural and mechanistic studies on eukaryotic UDP-galactopyranose mutases

    Oppenheimer, Michelle LynnVirginia Tech2012

    … structure these eukaryotic UGMs were examined by steady-state kinetics, rapid-reaction kinetics, trapping of reaction intermediates, fluorescence anisotropy, and X-ray crystallography. The mechanism first involves reduction of the required flavin by NADPH, followed by UDP-Galp binding and …

    vt Repository record

  12. Structure and Mechanism of a Eukaryotic FMN Adenylyltransferase

    Huerta, Carlos, Jr.2010

    … 1.20-1.95 Å, capturing the enzyme active site states prior to and after catalysis. These structures reveal a novel flavin-binding mode and a unique enzyme-bound FAD conformation. Comparison of the bacterial and eukaryotic FMNAT provides a structural basis for understanding the convergent …

    utswmed Repository record

  13. Understanding the mechanism of androgen biosynthesis

    Gregory, Michael CarltonUniversity of Illinois at Urbana-Champaign2017

    … elusive. This dissertation documents the steady state kinetics of CYP17A1 catalysis as well as detailed characterization of its heme environment in the ferric, oxy-ferrous, and peroxo- states by resonance Raman spectroscopy in order to answer this essential question. Presence of an inverse …

    uiuc Repository record

  14. Protein Dynamics and its Correlation to Protein Activity and Stability

    Clement, DavidThe University of Waikato2009

    … The inhibitory power of MTX was also examined by steady state kinetics and isothermal titration calorimetry. The Ki for MTX was found to be in the nanomolar range Ki= 10.9 nM. Using isothermal titration calorimetry, the binding thermodynamic signature between MTX and DHFR was characterized. The …

    waikato-masters Repository record

  15. Mechanisms of Flavin-Dependent Monooxygenases Involved in Natural Product Chemistry

    Johnson, Sydney B.Virginia Tech2024

    … of cremeomycin. For each enzyme, the steady-state kinetics have been determined using an oxygen consumption assay and the rapid-reaction kinetics were measured using anaerobic time-resolved spectroscopy. All three enzymes feature a fast flavin reduction step and a slow flavin …

    vt Repository record

  16. Biochemical mechanisms for selectivity governing RNA and DNA replication

    Dangerfield, Tyler LaneThe University of Texas at Austin2021

    … T7 DNA polymerase, the SARS-CoV-2 pandemic devastated the world. I used techniques developed in my previous studies to characterize the RNA dependent RNA polymerase (RdRp) from SARS-CoV-2, a prime target for antiviral drugs to stop replication of the virus. I developed methods to express the tag …

    texas Repository record

  17. Spectroscopic characterization of iron-oxygen intermediates in human aromatase (CYP19A1)

    Luthra, AbhinavUniversity of Illinois at Urbana-Champaign2015

    … 70% of all breast cancer cases in the United States. Starting with its androgenic substrates, testosterone and androstenedione, CYP19A1 forms estradiol and estrone utilizing one molecule of atmospheric oxygen and two reducing equivalents in the form of NADPH. This is accomplished in a …

    uiuc Repository record

  18. Biophysical Investigation into the Protein Dynamics Governing the Allosteric Regulation of Plant and Animal 15-Lipoxygenases

    Roberts, DaniellaEast Carolina University2022

    Lipoxygenases (LOXs) are a family of enzymes found in plants, animals, fungi, and bacteria that catalyze the per-oxidation of polyunsaturated fatty acids. In plants, LOXs are involved in growth, development, and defense against pathogenic attacks. There are also multiple isoforms present in humans, …

    ecu Repository record

  19. Kinetics and Mechanism of Ozone Decomposition and Oxidation of Ethanol on Manganese Oxide Catalysts

    Li, WeiVirginia Tech1998

    … validity of the proposed sequence. The transient kinetics were found to be consistent with the steady state kinetics, and both were well represented by the two-step sequence, indicating that the proposed reaction sequence accurately described the mechanism of decomposition. The surface was found …

    vt Repository record

  20. Interactions of Smooth Muscle Myosin Filaments with Actin and Myosin Light Chain Kinase: Insights into the Mechanics of Smooth Muscle Contraction

    Haldeman, Brian D.2015

    … of the filamentous conformation of SMM on the kinetics underlying the interactions with MLCK and actin is unknown. The filamentous structure of unphosphorylated smooth muscle myosin is not stable under physiological conditions, so there is a lack of research studying filaments of smooth muscle …

    unr Repository record

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