Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 70 for “"ssNMR"”.
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Structural Studies of Lipid-Bound Apolipoprotein A-I and Nanodisc-Embedded Cyp3A4 by SSNMR
… of membrane proteins using solid-state NMR (SSNMR).
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Multinuclear Solid-State Nuclear Magnetic Resonance Spectroscopy of Microporous Materials
… from other techniques such as solid-state NMR (SSNMR) spectroscopy. This thesis is focused on the study of two types of microporous materials, metal–organic frameworks (MOFs) and titanosilicates, by multinuclear SSNMR spectroscopy in combination with quantum chemical calculations for …
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Methods for Increasing Sensitivity and Throughput of Solid-State NMR Spectroscopy of Pharmaceutical Solids
Solid-state nuclear magnetic resonance (SSNMR) spectroscopy has been demonstrated to be a powerful technique for investigating solid dosage formulations. SSNMR has the ability to determine physical form, molecular structure, and dynamics of a pure or formulated active pharmaceutical ingredient …
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Structure determination of proteins and protein aggregates by magic-angle spinning solid-state NMR
Solid state NMR (SSNMR) is a structure determination technique uniquely suited to study protein aggregates and fibrils. Unlike solution NMR or X-ray crystallography, SSNMR can obtain atomic resolution structural information on samples of protein fibrils which are insoluble and do not produce X-ray …
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Investigation of cell interfaces and strained tissues with solid-state NMR
Solid-state nuclear magnetic resonance (ssNMR) is an immensely powerful tool for structural biology, which can provide insights into the composition, structure, dynamics of biological components. In combination with <sup>13</sup>C amino acid enrichment of cell fibroblasts *in vitro*, ssNMR has been …
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Applications of DNP and solid-state NMR for protein structure determination
… (MAS) solid state nuclear magnetic resonance (SSNMR) is a developing method for determining the structures and studying the dynamics and functions of biological molecules. This method is particularly important for systems, such as amyloidogenic fibrous proteins, that do not crystallize or …
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Examining Molecular Interactions of Proteins by Isotopic Labeling, Sample Formulation and Solid-State NMR Spectroscopy
Advances in magic-angle spinning solid-state NMR (SSNMR) methods have led to several structural and functional studies of proteins. These methods can be extended to determine precise mechanistic details and molecular interactions of membrane or microcrystalline protein formulations. In this work, …
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Glycation of Extracellular Matrix: A Solid-State NMR Study
… using solid-state nuclear magnetic resonance (SSNMR) spectroscopy. The background and motivations of this project are outlined in chapter 1. Chapter 2 reviews the current understanding of the composition and organisation of the ECM, the structure of collagen and the critical roles collagen …
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High field DNP and cryogenic MAS NMR : novel instrumentation and applications
Solid State Nuclear Magnetic Resonance (ssNMR) spectroscopy has blossomed over the last two decades. As ssNMR is progressively applied to more challenging systems, the sensitivity remains one of its major limiting factors. Gyrotron based high-field dynamic nuclear polarization (DNP) permits …
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Solid-state NMR structural investigations of alpha-synuclein fibrils derived from Lewy Body dementia brain tissue
… been developed using data from solid-state NMR (SSNMR) or cryo-EM. Here, I present an atomic model of an α-syn fibril that was derived from a deceased patient that had LBD, based on data from SSNMR and transmission electron microscopy. This structure was solved using an automated algorithm for …
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Development and Application of Solid-State NMR Methods for Investigating Protein Structure and Dynamics
… nuclear magnetic resonance spectroscopy (SSNMR) is uniquely well-suited among spectroscopic and microscopy techniques for studying the structure and dynamics of biomacromolecules with atomic length-scale resolution. The development and application of advanced SSNMR techniques facilitates …
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Biomolecular Solid-State Nuclear Magnetic Resonance Methods for Spectral Assignment and High-Resolution Structure Determination of Proteins
… solid-state nuclear magnetic resonance (SSNMR) experiments for the elucidation of structure in microcrystalline proteins are presented, utilizing the streptococcal beta-1 immunoglobulin binding domain of protein G (GB1) as a model protein. Complete 13C and 15N chemical shifts are …
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Solid-state NMR of low-gamma nuclei and solid-state NMR probe design towards minimization of the perturbation of the applied magnetic field
… non-destructive solid- state NMR spectroscopy (SSNMR). The SSNMR approach can be used to obtain details about molecular- level structure andmetal-ligand interactions, leading to deeper understanding of structure-function-property relationships. In addition to experimental techniques, the design …
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Membranous sterol dynamics and interactions with amphotericin B
… well resolved. Here we utilize solid-state NMR (SSNMR) to probe the atomistic interactions of Chol with membranes and other related systems. Collaboration with synthetic and computational groups has enabled a powerful multidisciplinary approach to study these systems using SSNMR, biosynthesis, …
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Use solid-state NMR to study the molecular structures of disease-associated peptide aggregates
… lab uses solid-state nuclear magnetic resonance (ssNMR) to characterize the molecular structures of different types of aggregates formed by disease-associated proteins or peptides. In this thesis, we study three aggregate samples to reveal the structural information related to their molecular …
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A short & sweet story of CHO
<p>The solid state nuclear magnetic resonance (SSNMR) study of nearly intact plant cell walls along with the computational study of glucose and malonic acid are presented. Previously unassigned <sup>13</sup>C chemical shifts are presented for the various carbohydrates in the primary plant cell wall …
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Solid-state NMR studies of membrane proteins and membrane protein complexes
… membrane proteins. Conversely, solid-state NMR (SSNMR) has no inherent size limitation and does not require the use of crystals, which presents SSNMR with the unique capability to study membrane proteins in native environments at atomic-resolution. However, this technique is still a relatively …
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Structural determinants of cardiac light chain amyloidosis
… use of solid-state nuclear magnetic resonance (SSNMR) spectroscopy, which have revealed new insights on specific features of the fibril species. First, we report our findings on the 13C and 15N chemical shift assignments and sequence involvement of AL-09 VL fibrils, followed by subsequent …
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EPR and solid-state NMR studies on the mechanism of cytochrome bo3 ubiquinol oxidase from escherichia coli
… from C43(DE3) auxotrophs for solid-state NMR (SSNMR) experiments. The initial SSNMR spectra proved that clean isotope labeling at the selected amino acid types was achieved through the use of auxotroph strains. This selective labeling approach dramatically simplifies the SSNMR signals and …
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Implementation of Solution and Solid Sate Nuclear Magnetic Resonance (NMR) Spectroscopic Techniques for Quantitative and Qualitative Analysis of Molecular Species
… of solid-state NMR spectroscopy) is to apply SSNMR spectroscopy experiments in two projects to gain significant information about specific materials. In chapter 6, some main concepts of SSNMR spectroscopy are discussed as well as some basic SSNMR experiments. In chapter 7, boron carbide thin …
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