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Showing 1 to 12 of 12 for “"spore coat"”.
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Engineering spores to display g protein-coupled receptors for directed evolution
… a heterologous protein on the Bacillus subtilis spore coat. Human parathyroid hormone receptor (huPTH1R) is used as a model system. HuPTH1R is a GPCR, which is vital in regulating calcium and phosphate levels in the blood. Molecular biology is used to create the plasmid pDG1730 huPTH1R-CotC that …
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Protein engineering of cota laccase by using bacillus subtilis spore display
Spore display offers advantages over more commonly utilized microbe cell-surface display systems. For instance, protein-folding problems associated with the expressed recombinant polypeptide crossing membranes are avoided. Hence, a different region of protein space can be explored that previously …
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Polypeptide (dis)assembly in bacterial spores and microalgae
The ability to form an endospore allows bacteria of certain genera to survive unfavourable environmental conditions. This durability is known to be related to the properties of the concentric protein shells that surround the spore, forming a distinct layered coat. This thesis begins with …
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Molecular analysis of GerP and spore-associated proteins of Bacillus cereus
Spores of various strains of Bacillus cereus are the causative agents of emetic and diarrheal foodborne illnesses. Typically, spores will survive thermal treatments that destroy vegetative cells, and then go on to germinate to form the vegetative cells that are associated with toxin production. The …
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Phenotypic and Genotypic characterisation of Bacillus sporothermodurans
… treatment of milk that is able to destroy non- sporeformers and sporeformers. The UHT process uses a temperature of 135-140°C for 1-2 seconds. Bacillus sporothermodurans is the only known species whose spores are able to survive the UHT treatment. With companies having the disadvantage of not …
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Exosporium Morphogenesis in Bacillus Cereus and Bacillus Anthracis
<p>Bacillus cereus is a Gram-positive spore-forming bacteria that can cause food poisoning and its close relative, Bacillus anthracis is the etiological agent of anthrax. In both cases, the spore, a differentiated cell type in a dormant state, starts the infection process. Thus, the exosporium, …
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A comparison of sulfhydryl active compounds on activation and germination of Bacillus thuringensis spores
Spores of Bacillus thuringiensis HD-1 and Bacillus cereus 569 were incubated in various sulfhydryl active compounds in order to compare the effects of these compounds on activation and germination. The sulfhydryl active compounds used were oxidized lipoic acid and cystine (S-S), cysteine HCl and …
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Functionalization of phage endolysins to treat infections of spore-forming Clostridia bacteria in the era of antibiotic resistance
… not only because they form highly resistant endospores but because they produce toxins and exhibit antibiotic resistance. *Clostridioides difficile* is a nosocomial pathogen responsible for thousands of deaths every year, whereas *Clostridium perfringens* is a common cause of food poisoning and a …
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Development of Bacillus subtilis spores and cells for surface display of proteins
… bacterial and eukaryotic cells and bacterial spores. Among these bioparticles, the endospore from B. subtilis has advantages, including feasibility of production, safety feature, the robustness of the bacterial spore allowing storage in the desiccated form, a technological platform supported …
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The Control of Spore Germination in Anemia Phyllitidis (L.) Swartz and Other Ferns
<p>Many fern spores require light for germination. In one family of ferns, the Schizaeaceae, the gibberellins overcome this light requirement and induce dark-germination. Fern gametophytes produce antheridium-inducing substances. The Anemia substance, antheridogen-B, is demonstrated to induce …
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Dictyostelium als Wirtsmodell und Funktionsanalyse des Virulenzfaktors Mip aus Legionella pneumophila
… Fusion von Vesikeln), Discoidin I, CotB (spore coat Protein SP70) und die lysosomale α-Mannosidase. Mit Hilfe von Homologie-Suchen konnte weiteren unbekannten Proteinen eine Funktion zugeteilt werden. Hierzu zählen die Chaperone ClpB (heat shock protein Hsp104), β’-COP (coat protein) und …